[Changes in the initial platelet aggregation during storage of the platelet concentrate and exposure to the myeloperoxidase reaction products].
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Animals↗
Biomedical subjects
V V Berzhitskaia
Publications and source records attributed to V V Berzhitskaia.
[Some factors of collagen aging factors in vivo and in vitro].
The literature data on different factors of collagen aging, the main connective tissue protein, have been analyzed. Mechanisms of reactions that are involved in both old aging and non-enzymatic course of protein aging are discussed. Factory influencing, non-enzymatic protein glycation during-related pathology and some ways for its inactivation by different substances are reviewed.
Aging↗
[Difference in inhibitory actions of products of the myeloperoxidase- catalyzed reaction on initial aggregation of activated platelets].
Inhibition of the initial aggregation of isolated rabbit platelets was studied on two types of their stimulation. The stimuli were either ADP, which was also capable to induce cell rounding, or adrenaline, and CaCl2, which acted without cell rounding. When platelets are stimulated by adrenaline or calcium chloride, sodium hypochlorite enhances the anti-aggregatory effect: at a concentration of about 10 microM the aggregation is reduced by 50%. This inhibitory effect is 1.5-3 times the ADP-stimulated inhibition of the aggregation of platelets or the inhibition of platelet aggregation by N-chloralanine under activation of platelets by any stimulus. Dithionitrobenzoic acid, which is a widely used reactant for sulfhydryl groups, also suppresses the platelet aggregation. Its inhibitory effect is similar to the of antiaggregatory action of sodium hypochlorite on platelets. It is not excluded that the enhanced antiaggregatory effect of sodium hypochlorite is due to an alteration of calcium channels in platelet plasma membranes, which occurs with the involvement of sulfhydryl groups.
Adenosine Diphosphate↗
[Changes in small-angle light scattering by platelets during their activation and aggregation].
Small-angle light scattering (0.5-7 degrees) platelets is enhanced upon the adenosine-5'-diphosphate-induced formation of microaggregates of platelets. The intensity of the scattered light, measured within a short period of time after stimulation of platelets, may by used as a nephelometric quantitative index of their initial aggregation. The light transmittance of platelet suspensions decreases after stimulation due to both the rounding of cell body and the initial aggregation of cells. Sodium hypochlorite at concentrations corresponding to its content in tap water inhibits the Ca(2+)-induced aggregation of isolated rabbit platelets. The tap water causes the transition of platelets to a state that is refractory with respect to CaCl2.
Animals↗