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Biomedical subjects

V Sticht-Groh

Publications and source records attributed to V Sticht-Groh.

At least 37 records · Page 2Linked to original sources
PubMed1993

Investigations into the growth of Mycobacterium leprae in a medium with palmitic acid under different gaseous environments.

Low oxygen tension has often been considered important for the growth of Mycobacterium leprae. Palmitic acid has been suggested as the oxidizable substrate for the in vitro cultivation of leprosy bacilli. The combined effects of palmitic acid and various known gas mixtures on the in vitro growth of M. leprae were investigated. When palmitic acid was included in the medium an optimal growth in both liquid and solid media was obtained between 16 to 20 weeks of incubation under gas mixtures containing 2.5 or 5% O2 and 5 or 10% CO2 as well as air. The use of different gas mixtures is tedious, time consuming and laborious. Since the cultures incubated under air gave the same cell yield as obtained when incubated under optimal gas mixtures, air alone can be used for the in vitro cultivation trials of M. leprae when palmitic acid is included in the culture medium.

Adenosine Triphosphate↗
PubMed1992

Use of a different buffer system in the phenolic glycolipid-I ELISA.

By changing the buffer system in the phenolic glycolipid-I (PGL-I) enzyme-linked immunosorbent assay (ELISA) the sensitivity of the test was increased without altering its specificity. Using a Tris-HCl buffer, significant titers of > or = 1:300 were found in 53.1% of the sera in paucibacillary (PB) and 98.0% of the sera in multibacillary (MB) groups of patients. Titer levels were also significantly increased. In the PB group of patients with Tris-HCl, the highest titer detected was 1:1200; in the MB group of patients, 1:76,800. Through this modification of the buffer system a more sensitive test was obtained thereby increasing the detectable level of PGL-I antibodies in both the PB and MB groups of patients.

Antibodies, Bacterial↗
PubMed1989

Meningitis due to Borrelia burgdorferi in the initial stage of Lyme disease.

Borrelia burgdorferi (B.b.) was isolated from cerebrospinal fluid from two children with aseptic meningitis. Serology for B.b. was initially negative or borderline and became positive during the subsequent course. Children with aseptic meningitis of unknown origin should be re-evaluated within 1 month to detect possible increasing antibody titres to B.b. Aseptic meningitis may be a stage 1 manifestation of Lyme disease.

Adolescent↗
PubMed1989

Autoimmunity in Lyme disease: molecular cloning of antigens recognized by antibodies in the cerebrospinal fluid.

In inflammatory disease of the central nervous system (CNS), oligoclonal bands of immunoglobulin with restricted heterogeneity can often be observed in cerebrospinal fluid (CSF) samples. These antibodies can be directed against the disease inducing pathogen or might be autoreactive and involved in the process of brain inflammation and demyelination. We used a molecular biology approach to characterize these antibody responses in patients with Lyme disease. This disorder is caused by infections with the spirochete Borrelia burgdorferi which is transmitted by ticks. Lyme disease can be associated with neurological symptoms due to inflammation of the central and peripheral nervous system. Phage lambda gtll expression libraries from B. burgdorferi and human brain were screened with cerebrospinal fluid antibody probes from patients with Lyme disease. We obtained recombinant phage clones encoding antigenic proteins from both B. burgdorferi and human CNS libraries. Thus, in this study two patients with chronic Lyme disease produced antibodies against recombinant B. burgdorferi as well as against CNS proteins, and the generation of this transient autoimmune response might be essential to the development of demyelinating disease.

Antibodies, Bacterial↗
PubMed1988

Borrelia burgdorferi--specific and autoreactive T-cell lines from cerebrospinal fluid in Lyme radiculomyelitis.

In 3 patients with Lyme radiculomyelitis, cellular immune reactions of cerebrospinal fluid (CSF) lymphocytes were analyzed. Phenotypic analysis of CSF cells demonstrated that the majority were T cells (CD3+) of the helper/inducer subset (CD4+). These T cells were directly expanded from the CSF by limiting dilution. A total of 505 T-cell lines were tested for Borrelia burgdorferi (Bb)-specific proliferation and also partly tested for reactivity to a panel of central and peripheral nervous system antigens. Proliferative assays revealed 33 of them to be Bb specific, 16 to be specific for myelin basic protein, 16 to be specific for peripheral myelin, 1 to be specific for cardiolipin, and 2 to be specific for galactocerebrosides. The antigen-specific proliferation was restricted by autologous human leukocyte antigen (HLA) class II molecules. The majority of CSF-derived T-cell lines stained positively for CD3, CD4, and HLA class II antigens and negatively for CD8 (cytotoxic/suppressor subset). One T-cell line provided help for the production of specific IgG by autologous B cells and secreted gamma-interferon upon stimulation with Bb antigen in the presence of autologous antigen-presenting cells. These data show that in patients with severe neurological manifestations of late Lyme disease, not only Bb-specific T-cell lines but also T cells reactive to central or peripheral nervous system autoantigens can be found.

Adult↗
PubMed1988

Persistent intrathecal secretion of oligoclonal, Borrelia burgdorferi-specific IgG in chronic meningoradiculomyelitis.

In the cerebrospinal fluid IgG of five patients with lymphomeningoradiculitis (Bannwarth's syndrome) and radiculomyelitis studied by immunoblot technique an oligoclonal pattern was found. Most of these oligoclonal bands were specific for Borrelia burgdorferi. In patients suffering from chronic meningoradiculomyelitis, repeated CSF examination by this technique showed persistent secretion of identical IgG bands. Thus, the specific humoral immune response and the disease activity could be documented over the course of the disease.

Antibodies, Bacterial↗
PubMed1987

[Septicemia caused by enteritis due to Campylobacter fetus subspecies fetus].

In a 55 year old man with renal insufficiency requiring hemodialysis after bilateral nephrectomy who had been hospitalized with febrile illness and enteritis, Campylobacter fetus subspecies fetus could be isolated from the blood and feces. Antibodies against the homologous strain (isolated from the blood) could be detected in the serum by means of the Widal test and the indirect immunofluorescence test. Culturing of Campylobacter fetus subspecies fetus (especially from blood cultures) may be difficult because of the poor growth conditions for microaerophilic bacteria. It is quite possible that this organism occurs more frequently in blood and feces than suggested by the routine investigations carried out so far.

Campylobacter Infections↗
PubMed1984

Antibacterial activities of nitrothiazole against Campylobacter jejuni and Campylobacter coli.

Niridazole (Ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [MIC50], 0.0075 to 0.015 mg/liter). There were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (MIC50, 2 mg/liter). Nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (MIC50, 2 and 4 mg/liter, respectively). The nitrofuran derivatives, such as nitrofurazone and nitrofurantoin, were also less active (MIC50, 1 mg/liter). Niridazole and another potent nitrothiazole derivative killed the campylobacters rapidly at low concentrations. In contrast, much higher concentrations of metronidazole were required to achieve bactericidal values.

Anti-Bacterial Agents↗
PubMed1982

Campylobacter in healthy slaughter pigs: a possible source of infection for man.

Campylobacter were isolated from 103 of 173 (59 per cent) specimens of healthy slaughter pig faeces, washed intestines and water samples collected from a slaughterhouse and butcher's shop in West Germany. As most cases of human campylobacter enteritis are caused by Campylobacter jejuni, an attempt was made to find this organism among the isolates. Twenty-five out of the 103 strains (24 per cent) were identified as C jejuni. C jejuni was also isolated from salted water samples after overnight bowel storage in the butcher's shop, indicating that the customary salt preparation of the intestines did not eliminate all organisms present.

Animals↗