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Biomedical subjects

V Sharma

Publications and source records attributed to V Sharma.

At least 415 records · Page 23Linked to original sources
PubMed1984

Effect of estradiol on the oxalate metabolism in vitamin B6-deficient male rats.

Estradiol-implanted (EB) and sham-operated (SB) male rats (100-120 g Fody wt) were kept on a vitamin B6-deficient diet for 1 month along (Formula: see text) with their respective estradiol pair-fed (EPF) and sham-operated pair-fed (SPF) controls. Pyridoxine-deficient animals had higher kidney weights and increased activities of liver glycolic acid oxidase (GAO) and glycolic acid dehydrogenase (GAD) as compared to their pair-fed control animals. Kidney weights, GAO, and GAD levels in these animals are negatively correlated with erythrocyte alanine transaminase (EALT) levels, indicating that pyridoxine status regulates these two major enzymes of oxalate biosynthesis. Estradiol-treated animals showed a lower reduction in EALT levels after feeding them a pyridoxine-deficient diet for 1 month as compared to untreated animals. Estradiol administration decreased GAO levels in both normal and pyridoxine-deficient animals.

Alanine Transaminase↗
PubMed1984

Influence of growth temperature on the development of Escherichia coli polysaccharide K antigens.

The established seventy-one Escherichia coli polysaccharide capsular K antigenic test strains were examined for the development of the K antigen after growth at 37 degrees C and 18 degrees C. Twenty-eight K antigens were not detectable after growth of bacteria at 18 degrees C, while the remaining 43 antigens were developed at both temperatures. Most of the temperature-dependent antigens belonged to electrophoretically fast-moving K polysaccharides of rather low molecular weight, characteristically found among the common K antigens from extraintestinal disease isolates. Lipopolysaccharide O antigens were developed at both growth temperatures.

Antigens, Bacterial↗
PubMed1984

Intestinal oxalate uptake in castrated male and female rats: evidence for altered brush border membrane composition.

The intestinal uptake rate of oxalate (mumoles/h/g tissue wt.) in castrated male (CM) rats, CM rats administered estradiol, and female (F) rats was 1.8, 1.4 and 1.3 times higher than that of male rats, whereas castrated female (CF) rats and CF rats administered testosterone absorbed oxalate at a rate similar to F rats, thereby, suggesting that gonadectomy affected intestinal uptake of oxalate only in male rats The intestinal oxalate uptake rate in all the groups increased linearly with increasing oxalate concentration (0.1- 6.0 mM). Chemical composition of brush border membrane showed significant changes in the sialic acid, phospholipid and cholesterol content following castration, which may lead to ultrastructural changes in the membrane thereby, increasing the absorption of oxalate.

Animals↗
PubMed1984

Mineral seal oil excreted in urine.

There is a paucity of reported cases demonstrating the gastrointestinal absorption and renal excretion of mineral seal oil, a petroleum distillate. In 1972, a teenager attempted suicide by purposely ingesting an entire 8 ounce bottle of furniture polish containing 99% mineral seal oil. She arrived in the F.R. two hours post-ingestion and was lavaged with two liters of normal saline and given two ounces of mineral oil and 20 ml of 50% magnesium sulfate by the tube. The stomach contents were yellow, thick, oily, and smelled like furniture polish. She was admitted for psychiatric evaluation and observation for the ingestion. The patient did not suffer any respiratory or CNS complications. However, she excreted oil droplets which coalasced to form an oil layer in her urine! She suffered no kidney damage or abnormality as could be detected by routine renal function studies. Pictures revealing oil in the urine will be shown.

Child↗
PubMed1984

Synthesis and central nervous system activities of certain fluorine-containing 3-substituted indol-2-ones.

Several fluorine-containing 3- aroylmethyl -3-hydroxyindol-2-ones (4a-g), 3- aroylmethyleneindol -2-ones (5a-g) and 3- aroylmethylindol -2-ones (6a-g) were synthesized from the corresponding fluorine-containing indole-2,3-diones and appropriate ketones. The compounds were characterized by spectral studies. Representative compounds of each series were tested on mice for CNS activities, viz. analgesic and anticonvulsant and the effects were also observed against amphetamine-induced stereotypy, on conditioned avoidance response and on potentiation of pentobarbitone sodium hypnosis. Of the compounds examined, the greatest degree of activity was observed in 3- aroyl - methyleneindol -2-ones with a pronounced unsaturation in the system.

Analgesics↗
PubMed1983

Breakdown product of factor B as an index of complement activation in lepromatous leprosy and its relation with bacillary load.

Serum complement profile studied in 50 lepromatous leprosy patients with various bacillary loads demonstrated significantly decreased C3 levels in patients with high bacteriological index (2+ to 4+) as compared with those with lesser bacterial load. In contrast, mean serum levels of C1q and C4 components remained unchanged. The concentration of factor B breakdown product (Ba) and its ratio to factor B increased with the bacterial density and more so in patients with erythema nodosum leprosum. A significant negative correlation was found between serum C3 and Ba levels in most lepromatous patients. Analysis of the data suggested the alternative pathway as the possible mechanism of complement activation in lepromatous leprosy.

Adult↗
PubMed1983

Feeding, illness and nutritional status of young children in rural Gujarat.

Eighty children, aged 0-18 months, were studied over a period of 6 months. Information was obtained from monthly interviews with mothers, when the children were also weighed. Feeding practices, incidence of illness and weight gain were described. The children who were nutritionally most at risk were identified. The average weight of the children was 1 kg less than the NCHS international reference by 3 months, 1.7 kg by 9 months. The majority of mothers did not introduce foods other than breast milk before 9 months. The children from labourers' households gained less weight between 3 and 6 months, had a higher prevalence of malnutrition and more reports of illness than children from non-labourers' households. The implications of these results for workers in child health and nutrition programmes are discussed.

Breast Feeding↗
PubMed1982

High incidence of IgG class of Epstein-Barr virus capsid antibody in Indian patients of lepromatous leprosy.

Low levels of Epstein-Barr virus capsid (EBVC) antibody of the IgG class were detected in the sera of 19 of 23 (82.6%) lepromatous leprosy patients and six of 38 (16%) healthy controls. In contrast, heterophile antibody was found in only six of 43 (14%) lepromatous patients and three of 41 (7%) normal subjects. Overlap of the two types of antibody occurred only in one normal serum. It is inferred that the presence of EBVC antibody against an unbiquitous virus in lepromatous patients who often suffer from impairment of cell-mediated immunity might be due to past infection leading to persistence of the virus in their lymphoid cells and subsequent production of specific anti-viral antibody. Further, the striking finding of low incidence and titre of EBVC antibody in the normal Indian adults is consistent with the rarity of EB virus-associated disease, such as Burkitt's lymphoma, nasopharyngeal carcinoma and infectious mononucleosis in India.

Adolescent↗
PubMed1981

Interactions of hemoglobin S with the red cell membrane.

Significant differences were observed in the binding of hemoglobin A and hemoglobin S to normal red cell membranes containing the fluorescent chromophore 12-(9-anthroyl) stearic acid. Deoxyhemoglobin S had a greater affinity for the membrane than did deoxyhemoglobin A and part of the binding of deoxyhemoglobin S appeared to be irreversible. When the hemoglobin binding site in red cell membranes, Band 3, was blocked with with glyceraldehyde-3-phosphate dehydrogenase, neither hemoglobin S nor A was bound. It is suggested that the binding sites for hemoglobin S to normal red cell membranes involves two steps: (1) the binding of hemoglobin to Band 3 in which increased binding reflects the greater positive charge followed by (2) a component of irreversible binding, believed to reflect an interaction between a hydrophobic area of hemoglobin S and red cell lipids.

Erythrocyte Membrane↗