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Biomedical subjects

V Schmid

Publications and source records attributed to V Schmid.

52 records · Page 3Linked to original sources
PubMed1987

Cell cycles and in vitro transdifferentiation and regeneration of isolated, striated muscle of jellyfish.

Isolated, mononucleated, cross-striated muscle cells of a medusa can transdifferentiate in vitro to various new cell types and even form a complex regenerate. The transdifferentiation events follow a strict pattern. The first new cell type resembles smooth muscle and is formed without a preceding DNA replication. This cell type behaves like a stem cell and by quantal cell cycles produces all other new cell types. Some preparations develop an inner and an outer layer separated by a basal lamella. Formation of these layers does not depend on DNA replication. When layers do not form, each division results in nerve cells and smooth muscle cells. If separation into layers occurs, then a regenerate will be formed, and in the course of only two cell cycles all necessary cell types to form a functional regenerate will differentiate.

Animals↗
PubMed1987

In vitro transdifferentiation of striated muscle to smooth muscle cells of a medusa.

Mononucleated striated muscle cells can be isolated from anthomedusae and cultivated in artificial seawater. In the cultivated muscle the differentiated state is maintained and DNA synthesis is not observed. The isolated striated muscle can be activated by collagenase treatment to transdifferentiate into various new cell types. Between the second and third day following collagenase treatment DNA synthesis is initiated, and mitosis and de novo flagellum formation occur in the isolated muscle. Under these circumstances all isolated striated muscle fragments produce both smooth muscle cells and y-cells (Schmid and Alder, 1984). In experiments, in which either transcription (actinomycin D) or translation (cycloheximide) is inhibited, the activated striated muscle cells do not transdifferentiate but maintain their differentiated state. Inhibition of DNA replication (aphidicolin), however, results in uniform transdifferentiation of striated muscle to smooth muscle cells in the absence of y-cell types (Schmid and Alder, 1984). The fluorescence stain NBD-phallacidin is used to monitor the characteristic change of F-actin pattern of these isolates.

Actins↗
PubMed1984

The influence of extracellular matrix on reversible gap junction formation in vitro.

In vivo gap junctions (gj) are common in the subumbrellar plate endoderm of anthomedusa. When isolated and cultivated in artificial sea water the tissue, consisting of one cell type only, forms a spheroid in which all gap junctions disappear. Gap junction (gj) formation can, however, be induced by attachment and consecutive spreading of the endodermal tissue (spheroid) on stretched extracellular matrix (ECM) material isolated from the polyp stage (with Ca2+-Mg2+-free sea water, without EDTA). Formation, and loss of gj is reversible and strictly corresponds with the alteration from the monolayer 'spread' (on stretched ECM) to 'spheroid' arrangement (no ECM) of the endodermal cells. The functional competence of induced gj is ascertained by injection of Lucifer Yellow, and the transfer of the dye is used to map the pattern of communication. The experimental conditions that result in gj formation simulate the in vivo situation of the endoderm. The influence of the ECM on gj formation, and the structural organization of the isolated endodermal tissue in this well defined in vitro system are discussed.

Animals↗
PubMed1984

Isolated, mononucleated, striated muscle can undergo pluripotent transdifferentiation and form a complex regenerate.

Isolated, mononucleated, cross-striated muscle of a medusa can be activated by collagenase treatment to transdifferentiate completely to various new cell types and to regenerate autonomously the sexual (without gametes) and feeding organ of the animal. Under these circumstances all isolated muscle fragments produce smooth muscle cells and a glandular cell type (y-cells). When culture conditions are appropriate, endoderm is also formed, followed by regeneration of a complex organ of seven or eight new non-muscle cell types, including nematocytes, digestive, secretory, gland, interstitial, and presumably nerve cells.

Animals↗