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Biomedical subjects

V Rzetelny

Publications and source records attributed to V Rzetelny.

16 recordsLinked to original sources
PubMed1999

Biochemical alterations secondary to disuse atrophy in the rat's serum and limb tissues.

Disuse atrophy has been the subject of research studies of an animal model in which single-limb immobilization induces atrophic changes in the immobilized limb. These reveal systemic changes in the experimental animals that go far beyond the local response expected in that situation and are not fully understood as yet. We therefore performed a biochemical study on the effect of hind-limb immobilization on the serum and tissues of rats. The experiment was carried out on 70 young Sprague-Dawley male rats. In one group of 35 rats, the left hind-limb was immobilized for 3 weeks. Another group of 35 rats served as controls. Serum total protein, albumin, urea, creatinine, and calcium were found to be reduced during immobilization. Serum cholesterol levels, on the other hand, increased to a considerable extent. No changes were recorded with phosphate, bilirubin, and magnesium. Serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were both reduced in activity. The activity of muscle aspartate aminotransferase (AST) and bone alkaline phosphatase (ALP) was also decreased. Lactate dehydrogenase (LDH) remained unchanged in both serum and muscle. We discuss our findings in the light of previous knowledge regarding the atrophic process.

Alkaline Phosphatase↗
PubMed1999

The effect of single hind-limb immobilization on the contralateral limb in the rat: a morphometric and biochemical study.

Disuse atrophy has been the subject of research studies that make the use of an animal model in which single-limb immobilization induces atrophic changes in the immobilized limb. The effect upon the nonimmobilized contralateral limb is, however, still unclear, and findings in the literature are inconclusive. We therefore performed a multidisciplinary study to clear this issue. The experimental population was 70 young male Sprague-Dawley rats. In one group of 35 rats, the left hind limb was immobilized for 3 weeks. Another group of 35 rats served as controls. limb and tibial bone weights (both "dry" and "ash") were found to be reduced in both hind limbs. Bone alkaline phosphatase (ALP) activity was likewise reduced in both limbs. We conclude that the contralateral hind limb in the rat is clearly subjected to atrophic changes that are similar in magnitude or severity to those experienced by the immobilized limb. We further discuss these findings in view of previous knowledge regarding the atrophic process.

Alkaline Phosphatase↗
PubMed1999

Microscopic alterations in the physis of long bones in response to hind limb immobilization in the rat.

BACKGROUND: The growth plate increases its activity in response to exercise. Likewise, decreased physical activity exerts a negative effect on bone growth and development, leading to rarefaction of the subepiphyseal bone. Limb immobilization inhibits the growth plate's activity, indirectly shown by a recorded arrest in longitudinal growth of the long bones. However, there is no direct evidence concerning the growth plate itself. OBJECTIVE: To determine whether the growth plate exhibits measurable microstructural changes in response to decreased levels of physical activity. METHODS: Histomorphometric analysis was used to qualitatively and quantitatively assess the changes in the epiphyseal plate in response to single hind limb immobilization in the rat. In 16 of 25 Sprague-Dawley male rats the left hind limb was immobilized for 3 weeks; the remaining 9 rats served as controls. The left proximal tibia of each animal was examined by computerized image analysis. RESULTS: There was a decrease in epiphyseal height, cell column density and subepiphyseal trabecular area--all indices of growth plate activity. Metaphyseal cortical thickness was also depressed, thereby confirming the efficacy of the immobilization method applied. CONCLUSIONS: Limb immobilization in the rat induces inhibitory histological changes in the epiphyseal growth plate, which are in contrast to the excitatory microscopic changes seen with exercise. These changes can be assessed quantitatively. Their potential for reversibility remains to be determined by future experiments.

Animals↗
PubMed1997

[Osteoid osteoma of the patella].

Osteoid osteoma is a primary, benign, bone tumor with a typical X-ray appearance. The diagnosis is usually straightforward when it involves the long bones of the leg. However it may occur in unusual locations, such as in flat and cancellous bones. The following case demonstrates the difficulty in diagnosing it in an extremely rare site. A 25-year-old woman with long-standing anterior, right knee pain had had 3 interventions: 2 diagnostic arthroscopies and a distal, femoral biopsy. Since there had been no improvement in her condition, the diagnosis of osteoid osteoma of the patella was suggested, supported by recent X-ray and scintigraphic findings. This rare condition was confirmed by biopsy of the upper pole of the patella, a procedure that was followed by complete recovery. 6 years later, she is now completely free of pain and has no clinical or X-ray evidence of recurrence. Review of the literature revealed only 5 previously reported cases of osteoid osteoma of the patella. All were diagnosed relatively late and in 1 the tumor was diagnosed only after total patellectomy. Diagnosis of these rare cases that mimic much more common causes of patello-femoral pain demands a high index of suspicion.

Adult↗
PubMed1994

Osteoid osteoma of the acetabulum. A case report.

The acetabulum is a very rare location for osteoid osteoma. Reported herein is the case of a 21-year-old man who had nonspecific hip pain. Preoperative diagnosis was made 2 months after the first visit. Excisional biopsy was performed through the inner wall of the acetabulum. The patient reported complete relief from pain and there has been no evidence of recurrence in 8 years of followup.

Acetabulum↗
PubMed1993

Arthroscopic adhesiolysis of the knee joint in arthrofibrosis.

Arthroscopically assisted adhesiolysis is a relatively new method for the treatment of arthrofibrosis of the knee. It is as effective as other known techniques and has the advantage of being minimally traumatic to the joint and the surrounding soft tissues. The operative method is presented here, together with three case reports of patients representing part of a series of 10 patients treated in our ward by arthroscopic adhesiolysis. Results, which were very favorable--average flexion improved 72 degrees and average extension improved 12 degrees--were stable over time, and all patients were pain-free at follow-up. Our experience supports previous positive reports concerning this additional mode of treatment for arthrofibrosis.

Adolescent↗