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Biomedical subjects

V Rizzoli

Publications and source records attributed to V Rizzoli.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

S-acetyl- and S-phenylacetyl-glutathione as glutathione precursors in rat plasma and tissue preparations.

S-acetyl- and S-phenylacetyl-glutathione derivatives were synthesized by using a new procedure. The derivatives were incubated with rat plasma and red blood cells, and also with cytosol from rat liver, kidney and heart, or tissue slices from rat heart, kidney and liver. A limited hydrolysis of the compounds occurs in plasma, whereas hydrolysis occurs to a larger extent in tissue cytosols. Both purified and crude gamma-glutamyl-transpeptidase from different sources recognized the S-acetyl- and S-phenylacetyl derivatives as substrates. Intracellular glutathione increases after incubating the derivatives with red blood cells. A potential role of S-acetyl- and S-phenylacetyl-glutathione in replenishing cells with exogenous glutathione is envisaged.

Animals↗
PubMedDate not supplied

Human peripheral blood mononuclear cell subfractionation using counterflow centrifugation elutriation.

BACKGROUND: Counterflow Centrifugation Elutriation (CCE) can be used to separate different amounts of cells into distinct subpopulations on the basis of different sedimentation characteristics. It was the aim of this study to evaluate CCE as a technique for fractionation of normal human peripheral blood. METHODS: Human peripheral blood mononuclear cells (PBMC) were separated into three fractions by means of CCE. RESULTS: The first two fractions, eluted at a flow rate of 21 ml/min and 25 ml/min, respectively, were composed of lymphocytes with less than 2% and 4% contaminating monocytes. The cells in these fractions were capable of responding to T-cell mitogens, such as phytohemagglutinin (PHA). The third fraction, collected at the termination of the run, was composed predominantly (greater than 85%) of large monocytes that strongly labelled leu-M3 (CD 14); these cells were unable to respond to PHA stimulation. The cell recovery after CCE was considerably high (greater than 93%), and all fractions retained high cell viability (greater than 96%) and were available for further manipulation. CONCLUSIONS: In conclusion, our data demonstrate that CCE is a technique suitable for large-scale separations that makes highly purified cell fractions available for further manipulation.

Cell Separation↗
PubMedDate not supplied

Intensification in post-remission treatment of adult acute non lymphocytic leukemia.

In an attempt to reduce the risk of leukemic relapse, different post-remission intensifications based on high-dose Ara-C (HiDAC) and autologous bone marrow transplantation (ABMT) were evaluated in patients with acute non lymphocytic leukemia in first remission and compared as to response and toxicity. Between September, 1985 and May, 1987, 34 patients in complete remission were eligible for our study. Induction therapy consisted of one or two courses of daunorubicin (DNR) and Ara-C (schedule 3 + 7). Fourteen patients receiving intensive post-remission chemotherapy with DNR + Ara-C (schedule 2 + 5), HiDAC + DNR, and ABMT following pretransplant BAVC conditioning entered the first pilot study. A high toxicity was observed and only 5 of them completed the full treatment plan. Thus the second pilot study used a single post-remission intensive course with HiDAC + m-AMSA and ABMT following cyclophosphamide plus TBI or BAVC. This approach was more feasible. The preliminary results show the usefulness of intensive post-remission therapy: in fact, all patients but one who completed the treatment program are still in continuous complete remission. A large number of patients and a longer follow-up are required to draw final conclusions.

Adolescent↗