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Biomedical subjects

V Reddy

Publications and source records attributed to V Reddy.

At least 145 records · Page 8Linked to original sources
PubMed1985

The comparison of human lens crystallins using three monoclonal antibodies.

Three monoclonal antibodies against lens crystallin have been used to study the accumulation of specific polypeptides during development of the human lens. One antibody which recognizes an antigen common to three polypeptides with molecular weights close to 31,000 reacted equally well to the human lens cortex and nucleus and had a similar binding activity to proteins isolated from embryonic and older human lenses. This suggests that the antigen accumulates to the same degree in human lenses during development. The second monoclonal antibody to the 24,000 molecular weight gamma-crystallin showed increased binding at the older ages indicating the increased accumulation of this protein during lens development. The third monoclonal antibody to a beta-crystallin of 27,000 molecular weight showed little if any reactivity at the embryonic age and revealed a clear difference between the binding with cortical and nuclear protein at older ages. It appears that the 27,000 molecular weight beta-crystallin may not be synthesized in the embryonic human lens.

Adult↗
PubMed1985

Nutritional status and diarrhoeal morbidity: a longitudinal study in rural Indian preschool children.

Malnutrition and diarrhoeal diseases are important interrelated health problems of preschool children in the developing countries. For a prospective study to determine the effect of malnutrition on diarrhoeal morbidity 721 children under 5 years, living in two villages near Hyderabad were registered. The children were classified into different nutritional grades based on initial body weights and heights; and diarrhoea morbidity was recorded during the subsequent one year. The aetiology of diarrhoea was determined in a subsample. The incidence of diarrhoea was observed to be about 1.6 episodes/child/year and was similar in children with different nutritional grades. The percentage of episodes leading to severe dehydration was, however, significantly higher (P less than 0.05) in children with grade III malnutrition. The isolation of ETEC was also higher in this group indicating a greater bacterial load in the gut. Although pre-existing malnutrition did not affect the incidence of diarrhoea it seems to have a significant impact on the severity of the disease.

Age Factors↗
PubMed1984

Impact of massive doses of vitamin A on incidence of nutritional blindness.

In a longitudinal study designed to assess the impact of a massive-dose vitamin A programme on the incidence of keratomalacia, 50 000 preschool children in 450 slum areas in Hyderabad city (India) were given 200 000 IU of vitamin A once every 6 months. During the study period, the incidence of keratomalacia in areas covered by the programme decreased by about 80%, while in control areas the reduction was of the order of 20%. To test whether large doses of vitamin A supplements prevented keratomalacia, a case-control analysis was done, with patients with severe protein-energy malnutrition being used as controls. The high odds ratio clearly indicated that keratomalacia was more likely to occur in children not receiving supplements.

Blindness↗
PubMed1984

Occurrence and prevention of rheumatic fever among ethnic groups of Hawaii.

To assess the occurrence of acute rheumatic fever (RF) among the ethnic groups of Hawaii, the case records of hospitalized children with RF in Oahu were reviewed for the period from October 1976 to September 1980. One hundred four of the records met the modified Jones criteria. Incidence rates per 100,000 children were as follows: Japanese, 0; white, 9; Filipino, 9.1; Hawaiian and part-Hawaiian, 27.2; and Samoan, 96.5. Carditis was most common among Samoan children; it occurred in nine of 18 children. A streptococcal, throat culture program for children with respiratory infections was in progress in 60% of Oahu's public schools during this period of time. Children with positive cultures were excluded from school until the start of treatment. However, RF occurred with equal frequency in participating and nonparticipating schools. Rheumatic fever continues to be a substantial problem among Polynesian children in Hawaii, and it is apparent that the school-based primary prevention program used in Hawaii to control streptococcal disease has not altered the frequency of RF among them.

Adolescent↗
PubMed1984

The development of a monoclonal antibody to a human gamma crystallin.

A monoclonal antibody to human lens 24,000 molecular weight gamma crystallin has been produced in rat x mouse hybridoma cell lines. The antibody reacts specifically with the 24,000 molecular weight gamma crystallin and does not cross react with the 21,000 or the 19,000 gamma crystallins. The antibody reacts with protein from dog, mouse, monkey and bovine lenses but does not react with proteins from chick lens. The 24,000 molecular weight gamma protein increases from 2% of the soluble protein in the fetal lens to 14% in the 2 month old lens to 25% in the adult lens. No difference was observed in the amount of this crystallin between the nuclear and cortical regions of the adult lens. In one hypermature cataractous lens, the amount of this crystallin decreased to a value of about 5% of the total soluble protein.

Adult↗
PubMed1984

Effect of measles on the nutritional status of preschool children.

A total of 1426 children admitted to the hospital for measles were studied. Of these, 84 were followed up for a period of 6 months after they were discharged from the hospital. It was observed that about 23% of the cases developed secondary infection during the acute stage of measles. Diarrhoea and respiratory infection were the common complications. Some children developed corneal lesions during the acute stage. Serum vitamin A levels were significantly low in these children and they showed a good clinical response to vitamin A therapy. During the follow-up period the children suffered from frequent episodes of infections for nearly 6 months after the attack of measles and the weight gain was significantly lower compared to normal children. These observations suggest that measles plays a significant role in precipitation of malnutrition and blindness due to vitamin A deficiency in preschool children.

Age Factors↗
PubMed1982

Studies on vitamin D metabolism in malnourished children.

1. Basal levels of serum 25-hydroxy vitamin D (25-OHD) were estimated in ten normal children and twenty-seven children with protein-energy malnutrition. 2. Five normal children and fourteen malnourished children were administered a single massive dose of 15 mg vitamin D orally and the remaining children received oral supplements of 50 micrograms vitamin D daily for 20 d. Blood samples were obtained after 10 and 20 d of dosing and serum 25-OHD levels were repeated. 3. The basal serum 25-OHD levels were significantly lower (P less than 0.05) in malnourished children than in normal children. 4. Administration of a single massive dose of vitamin D as well as daily supplementation of small doses resulted in significant increases in serum 25-OHD levels within 10 d. However, the increase with a massive dose was of a higher magnitude. There was no significant difference between the normal and malnourished children. 5. The binding capacity of serum to 25-OHD was similar in both the groups. 6. These results suggest that vitamin D metabolism is not altered in protein-energy malnutrition.

Blood Proteins↗