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Biomedical subjects

V P Tumanov

Publications and source records attributed to V P Tumanov.

At least 19 recordsLinked to original sources
PubMed2006

Modern cell technologies in the treatment of patients with tympanic membrane injury inflicted by mine explosion.

We evaluated the efficiency of transplantation of cultured human allofibroblasts onto tympanic membrane damaged by mine explosion in combination with Tampograss dressing (Paul Hartman). Transplantation of cultured human allofibroblasts was effective in 100% cases. Application of the film with fibroblasts onto perforation occupying from 1/4 to 1/2 of the tympanic membrane was more effective by 15% (by 59% in subtotal perforation) than tympanoplasty with amnion membrane. The mean duration of tympanic membrane restoration after spontaneous healing and amnionoplasty is virtually the same, while transplantation of allofibroblasts accelerated the process in comparison with other groups in perforation of any size; in subtotal defect the duration of tympanic membrane restoration was shorter by 14 +/- 1 days.

Accidents, Occupational↗
PubMed2006

[Treatment of scalping type nasal wounds by transplantation of cultured human allofibroblasts and a Grasolind neutral bandage].

The aim of the trial was evaluation of efficacy of human cultured allofibroblasts transplantation and novel bandage Grasolind neutral (Paul Hartmann, Germany) on the wound with a large defect of nasal soft tissues. Biopsy findings at different stages of healing of the wound confirmed high regenerative properties of human allofibroblasts. The latter promoted reestablishment of full-thickness graft in minimal scarring. After use of allofibroblasts physiological desquamation started 13 days earlier than in the control group. A novel interactive bandage Grasolind (Paul Hartman, Germany) produced a positive effect as it did not damage newly formed epidermal cells which stimulated regeneration.

Adult↗
PubMed2005

Ultrastructural changes in neurons of medullary giant-cell nucleus in growing rat exposed to acute stress.

Ultrastructural transformations in neurons of the medullary giant-cell reticular nucleus of young rats during exposure to acute emotional and painful stress attest to activation of the protein synthesis system (nucleolar hypertrophy, well-developed elements of the granular endoplasmic reticulum, increased content of free ribosomes and polysomes) in the majority of cells and the appearance of organelle injuries in some these cells.

Animals↗
PubMed2001

Morphological changes in burn wounds after transplantation of allogenic fibroblasts.

Cultured allogenic fibroblasts labeled with fluorescent acridine orange and transplanted onto burn wound virtually completely disappear from the wound surface within 2 days (are destroyed). Study of wound morphology by light autoradiography and quantitative analysis showed a stimulating effect of transplanted fibroblasts on wound healing. This effect, irrespective of the initial state of the wound, is always directed at accelerated formation of connective tissue similar to the derma and capable of epithelialization.

Acridine Orange↗
PubMed1993

[Persistence of physiological hydrocephaly in newborns].

During intratubal development of the brain the reduction delay of the fetal ventricular system is marked as persistence of the physiological hydrocephalus (PPH). PPH was revealed in 26.9%, 30.3% and 21.9% observations of premature and mature infants. The criteria of PPH are the extending of the posterior cornua of lateral ventricle and the absence of the interthalamus commissure in normal indexes of mass and anatomically formed brain. In pathogenesis of PPH the influence of teratogenic factors which enables to put it down to the minimal brain dysplasia is possible. PPH, though it is not of a clinical importance itself, can be favourable background for the developing of the hydrocephalus, but in the majority of the cases during postnatal period it liquidates.

Brain↗
PubMed1993

[Morphological and computer-tomographic analysis of reparative regeneration of the tibia by distraction osteosynthesis].

Morphological & computerized tomography analysis of tibial reparative regeneration in distraction osteosynthesis was performed in 12 & 25 patients consequently. The authors discuss the correlation between the two kinds of the data & show that development of bone regeneration with restoration of anatomical form & structure of the reconstructing tubular bone occurs in the place of osteotomy.

Biopsy↗
PubMed1993

[Cultured human skin cells in the treatment of burns].

The work discusses the results of experience of long-year's standing of the staff of the Vishnevsky Institute of Surgery in the creation of a biological bandage with the use of cultivated human skin cells. A graft of cultivated human allofibroblasts was used for closure of burn wounds for the first time. Its efficacy was studied in 86 cases of burns. The use of cultivated allofibroblasts is indicated in bordering III ab degree burns and sluggishly healing donor areas formed after autodermoplasty. Study of the efficacy of cultivated allofibroblasts in the treatment of deep burns in combination with dermoplasty with a 1:6 perforated skin autograft are continued. The low cost and technical simplicity of the method allow cultivated fibroblast grafts to be recommended for wide clinical practice.

Biological Dressings↗
PubMed1993

[Use of cell cultures in the local treatment of burn wounds].

The article deals with the results of using cultivated allofibroblasts in topical treatment of burn wounds. Allofibroblasts obtained from the derma of burnt individuals were transplanted to the burn wounds of 28 patients. In 12 among 15 patients (80%) with IIIa-IIIb degree burns transplantation induced epithelialization of the burns in 12.1 +/- 2.4 days. Thirteen patients had deep burns, only in 3 of them with a circumscribed burn area transplantation proved effective. Complications of transplantation were encountered in 5 patients--rejection of the transplant in 2 and its suppuration in 3 patients. Allofibroblast transplantation may be applied as an independent method of treatment in IIIa, b degree burns.

Adolescent↗
PubMed1993

[Treatment of chronic non-healing donor sites: transplantation of cultured human fibroblasts].

The authors discuss the first results of the use of cultivated human allofibroblasts in the treatment of persisting wounds of the donor areas in 13 patients with deep burns and cicatricial deformities caused by the burns. Transplantation of cell cultures led to complete epithelialization of the wounds in 6-10 days. Complication, suppuration of the grafted material, was recorded in one case. Reaction of graft rejection was not encountered. The authors believe that the effect of the transplantation is due to stimulation of the epithelialization processes by the cultivated fibroblasts.

Burns↗
PubMed1992

[The dynamics of the extinction of brain cellular biosynthesis at different times after death].

The paper presents experimental data on RNA synthesis in neuronal, glial, endothelial cells of a dying organism. Under certain conditions it is possible to define labelled cells in the majority of the brain cells within post-death hours 1-4. This fact proves that genome is still functioning. Endothelial cells possess higher vital capacity. CNS neurons are capable of synthesizing RNA within the period of up to 4 hours after death of the organism.

Autoradiography↗