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Biomedical subjects

V P Iatsenko

Publications and source records attributed to V P Iatsenko.

23 records · Page 2Linked to original sources
PubMedDate not supplied

[Alloplasty of nerves using cryopreserved grafts subjected to total epineurectomy (experimental-clinical study)].

It was demonstrated in experiments on 24 dogs that microsurgical epineurectomy of a cryopreserved peripheral nerve improves the results of neuroplasty due to acceleration and increase of graft revascularization. From application of the developed method in 12 patients it was concluded that microsurgical alloneuroplasty with a cryopreserved graft may be the method of choice in cases in which autoplasty cannot be performed.

Adolescent↗
PubMedDate not supplied

[Growth of rat fibroblasts in tissue culture as affected by trypsin immobilized on polyurethane substrates].

The growth of fibroblasts of the rat subcutaneous tissue was studied as affected by different doses of trypsin, free and bound, on polyurethane substrates. The method of tissue culture provides essential criteria for estimating the degree of bioincompatibility of polymeric alloimplants in particular when immobilizing biologically active substances on them. Trypsin adsorbed on the polyurethane substrate diffusing gradually into the culture medium is established to inhibit the cell growth. Chemically bounded trypsin in the composition of the polymeric matrix at the early stages of cultivation stimulates for a long time the division of fibroblastic elements and further accelerates their degeneration. It is shown that on polymeric substrates containing no trypsin, the growth character and dynamics of the fibroblastic elements are similar on the whole to these indices for cultures grown in the plasm clot without the substrate.

Animals↗
PubMedDate not supplied

[Method for the quantitative study of the growth of fibroblastic cells in tissue culture].

The method is elaborated for a quantitative evaluation of hypodermic fibroblast element growth in tissue culture of albino rats. The method is based on determination of the growth activity and intensity for each of the explant growth zones (compact, necwork-like zones and zone of migrating elements). The method permits a rapid and an exact evaluation of the influence of different substances added to the culture medium.

Animals↗