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Biomedical subjects

V N Krylov

Publications and source records attributed to V N Krylov.

130 records · Page 8Linked to original sources

[Certain physico-chemical properties of bacteriophage phiKZ].

Using UV-spectroscopy and circular dichroism (CD) phi KZ bacteriophage and its DNA were investigated. From the value of optical densities in the 320--400 nm range the size of the phi KZ bacteriophage's head was determined; the diameter of phi KZ bacteriophage head was found to be equal to 1300 +/- 100 A. phi KZ bacteriophage DNA has block structure and the GC-pair content is equal to 43.8 +/- 0.3%. phi KZ bacteriophage CD spectrum has an unusual profile (in comparison with known bacteriophage CD spectra); this spectrum is similar to the CD spectra of DNA in polyethylene glycole solution. To our knowledge CD spectra of such type were not obtained for other bacteriophages.

Bacteriophages↗

[Effect of bee venom and its components on electrophoretic mobility of erythrocytes].

The change of electrophoretic mobility (EPM) of erythrocytes of donor blood caused by their incubations with the bee venom and its major components--melittin and phospholipase A2 was investigated. It was estimated that the bee venom reversibly decreased EPM of erythrocytes to the higher degree than its separate components. The reaction was completely abolished after preliminary processing of erythrocytes with neuroaminidase and partly after their processing with glutaraldehyde, but the effect of EPM increased under the conditions of the increasing of ATP concentration. Proceeding from the authors' data it was concluded that the major components of the bee venom successively interacted with glicocalyx elements and membrane of erythrocytes which evokes the charge redistribution in these blood cells.

Adenosine Triphosphate↗