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Biomedical subjects

V Mirkovitch

Publications and source records attributed to V Mirkovitch.

At least 73 records · Page 4Linked to original sources
PubMed1976

A glycoprotein precipitate in the blood vessels of kidneys stored in cold homologous blood.

Dog kidneys were stored three hours at 4 degrees C in heparinized homologous blood. Their histological examination revealed the presence of a homogeneous precipitate in the glomerular capillaries and afferent and efferent arterioles, and histochemical analysis indicated that it consisted of glycoprotein. It is tentatively suggested that the substance is cryoglobulin, a conclusion supported by the disappearance of the deposit on reimplantation of the kidney.

Animals↗
PubMed1976

[Prevention of diabetes in pancreatectomised dogs by autotransplantation of pancreatic tissue in the liver].

In 15 mongrel dogs, a mixture of exocrine and endocrine pancreatic tissue obtained by a simple procedure was implanted into the liver of the same animal through a branch of the portal vein. Five animals with partial pancreatectomy were used for a morphological study. In a second group of ten dogs, 5 delayed and 5 immediate pancreatectomies were performed. No diabetes appeared after the pancreatectomy. The subsequent blood glucose and insulin levels remained within or close to the normal range for several weeks in 9 animals and up to 10 months in a last one, still alive. In 9 out of 10 animals, the long term study was limited between 6 and 17 weeks by the development of a malnutrition syndrome with hepatic steatosis due to the lack of exocrine secretory function because of the pancreatectomy. The last animal still alive developed the malnutrition syndrome after a second complementary resection of a small pancreatic fragment left along the duodenum. This last animal without diabetes at the 45th week suggests that the endocrine function of the autotransplanted pancreatic tissue could be maintained over a long period of time.

Animals↗
PubMed1975

Protection of the intestinal mucosa during ischaemia by intraluminal perfusion.

The function of the intestinal mucosa immediately after one hour's ischaemia was examined by means of tests in vivo and in vitro. During the ischaemia, the intestinal loop was perfused with media of different compositions, in an attempt to assess which provided the best protection of the epithelium from the deleterious effects of the ischaemia. The absorption of water, ions and glucose was then monitored in vivo, and the uptake of phenylalanine and beta methyl-glucoside by slices of mucosa was determined in vitro. The unprotected mucosa loses all active transport capacity in vitro following one hour's ischaemia, and is the site of a pronounced loss of water and ions into the lumen in vivo. Glucose absorption in vivo is also abolished. If the loop is perfused during the ischaemia with glucose-containing Krebs bicarbonate buffer, much of the transport capacity in vitro is retained; the loss of ions and water is prevented, and glucose absorption in vivo occurs. Perfusion during the ischaemia with other media, such as isotonic mannitol, Krebs bicarbonate buffer, or Ringer-lactate solution, results in a marked protection of the mucosa, in comparison with the unperfused loop, but the effects are not as pronounced as those of the glucose-containing buffer. It is concluded that the act of perfusing the intestine is the most beneficial factor, but that the presence of glucose in the perfusate does afford added protection.

Animals↗
PubMed1975

The response of the dog colon mucosa one hour's ischaemia.

Segments of dog colon were subjected to one hour's ischaemia, and their morphology and function were studied either immediately after the ischaemia or 24 hours later. As functional tests, net sodium transport across the mucosa in vitro, the transport capacity in vitro for sugars and amino-acids, tissue respiration, lactate production, and the liberation of acid phosphatase were applied. Immediately after the ischaemia, all parameters differed significantly from the control mucosa: net sodium transport was abolished; amino-acid and sugar uptake, oxygen consumption and lactate production were reduced; and lysosomal enzyme release was increased. All parameters, except sugar uptake, had normalised 24 hours after the trauma. Histological examinations revealed much variation in the lesions occurring after the ischaemia, varying from slight epithelial desquamation to considerable mucosal destruction. In most cases, the mucosa had almost normalised 24 hours later. Morphometric analysis revealed decreased mean mucosal thickness immediately after the trauma, though 24 hours later, there was again no significant difference with the control group.

Acid Phosphatase↗
PubMed1975

Active amino-acid and sugar uptake by gall bladder epithelium in dog, guinea-pig and man.

Slices of dog gall bladder are capable of accumulating amino-acids and sugars against considerable concentration gradients across the luminal membrane of the cell. The epithelium of the common bile duct also absorbs these substrates. The transport systems are sodium-dependent, saturable and inhibited by ouabain and metabolic poisons. The specificity of the mechanisms is more reminiscent of kidney than of intestinal transport. Glycine is preferentially transported across dog gall bladder from the mucosa to the serosa, but the net flux is small. It is concluded that the contraluminal membrane of the epithelial cell might be relatively impermeable to the amino-acid. concentrative uptake also occurs in guinea-pig gall bladder; however it appears to be practically non-existent in rabbit tissue. Human gall bladders, obtained at random from the operating room, also displayed active accumulation. The gall bladder epithelium is remarkably resistant to anoxia. Furthermore, following obstruction of the common bile duct for 2 weeks in dogs, the gall bladder is still able to concentrate amino-acids and sugars, but the extent of the uptake is significantly reduced.

Amino Acids↗
PubMed1975

Function and structure of the mucosa of continent ileostomy reservoirs in dogs.

Continent ileostomy reservoirs were constructed in dogs. Nine to 29 days after formation of the pouches, they were excised together with a loop of adjacent ileum for biochemical and morphological assessment. The villous and epithelial cell heights were reduced, whereas villous width and mitotic index were increased. The mucosa of the pouch was able to absorb phenylalanine and Beta-methyl-glucoside against a concentration gradient, although this absorptive capaciti was lower than that of normal ileum. Similar considerations applied to oxygen consumption. The results indicate that the mucosa of the pouch undergoes transformation, but the intergrity of the epithelial layer is maintained.

Acid Phosphatase↗