Biomedical subjects

V Mayya

Publications and source records attributed to V Mayya.

2 recordsLinked to original sources

PubMed2006

Direct cancer tissue proteomics: a method to identify candidate cancer biomarkers from formalin-fixed paraffin-embedded archival tissues.

Successful treatment of multiple cancer types requires early detection and identification of reliable biomarkers present in specific cancer tissues. To test the feasibility of identifying proteins from archival cancer tissues, we have developed a methodology, termed direct tissue proteomics (DTP), which can be used to identify proteins directly from formalin-fixed paraffin-embedded prostate cancer tissue samples. Using minute prostate biopsy sections, we demonstrate the identification of 428 prostate-expressed proteins using the shotgun method. Because the DTP method is not quantitative, we employed the absolute quantification method and demonstrate picogram level quantification of prostate-specific antigen. In depth bioinformatics analysis of these expressed proteins affords the categorization of metabolic pathways that may be important for distinct stages of prostate carcinogenesis. Furthermore, we validate Wnt-3 as an upregulated protein in cancerous prostate cells by immunohistochemistry. We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types.

Amino Acid Sequence↗

PubMed2005

STAT module can function as a biphasic amplitude filter.

Signal transducer and actuator of transcription (STATs) are a family of transcription factors activated by various cytokines, growth factors and hormones. They are important mediators of immune responses and growth and differentiation of various cell types. The STAT signalling system represents a defined functional module with a pattern of signalling that is conserved from flies to mammals. In order to probe and gain insights into the signalling properties of the STAT module by computational means, we developed a simple non-linear ordinary differential equations model within the 'Virtual Cell' framework. Our results demonstrate that the STAT module can operate as a 'biphasic amplitude filter' with an ability to amplify input signals within a specific intermediate range. We show that dimerisation of phosphorylated STAT is crucial for signal amplification and the amplitude filtering function. We also demonstrate that maximal amplification at intermediate levels of STAT activation is a moderately robust property of STAT module. We propose that these observations can be extrapolated to the analogous SMAD signalling module.

Cell Physiological Phenomena↗