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Biomedical subjects

V Marinescu

Publications and source records attributed to V Marinescu.

At least 19 recordsLinked to original sources
PubMed2001

Antioxidants attenuate early up regulation of retinal vascular endothelial growth factor in streptozotocin-diabetic rats.

AIMS/HYPOTHESIS: A strong positive correlation has been found between lipid peroxidation product and vascular endothelial growth factor concentrations in the vitreous of patients with proliferative diabetic retinopathy. To establish a causal relation between diabetes-associated enhanced oxidative stress and vascular endothelial growth factor production, we evaluated two antioxidants, DL-alpha-lipoic acid and taurine, on retinal vascular endothelial growth factor protein and mRNA expression and on parameters of oxidative stress in streptozotocin-diabetic rats. METHODS: Our experiments were on control rats and streptozotocin-diabetic rats with a 6-week duration of diabetes, treated with or without DL-alpha-lipoic acid (100 mg x kg(-1) x d(-1), i.p.) or taurine (1% in the diet) starting from induction of diabetes. Vascular endothelial growth factor protein in retinal homogenates was assessed by sandwich ELISA with an affinity-purified polyclonal antibody and vascular endothelial growth factor mRNA by ribonuclease protection assay. Retinal lipid peroxidation products i.e. malondialdehyde plus 4-hydroxyalkenals were quantified with N-methyl-2-phenylindole. Retinal reduced and oxidized glutathione, ascorbate, dehydroascorbate, and sorbitol pathway intermediates were measured spectrofluorometrically, and taurine by reverse-phase HPLC. RESULTS: Vascular endothelial growth factor protein concentration (means +/- SD) was increased in diabetic rats compared with control rats (33+/-7 vs 19+/-5 pg/mg total protein, p < 0.01) This increase was attenuated by taurine (26+/-8, p < 0.05) and prevented by DL-alpha-lipoic acid (21+/-4, p < 0.01). Vascular endothelial growth factor mRNA abundance was reduced by 1.4-fold in diabetic rats compared with control rats and this decrease was attenuated but not completely prevented by both antioxidants. Malondialdehyde plus 4-hydroxyalkenal concentration was increased in diabetic rats compared with control rats, and both antioxidants arrested accumulation of lipid peroxidation products. Taurine, reduced glutathione, oxidized glutathione, ascorbate, dehydroascorbate and sorbitol pathway intermediate concentrations as well as oxidized glutathione/reduced glutathione and dehydroascorbate/ascorbate ratios were similar in control and diabetic rats treated with or without taurine. CONCLUSION/INTERPRETATION: Oxidative stress is directly involved in up regulation of vascular endothelial growth factor protein in the retina during early diabetes.

Alternative Splicing↗
PubMedDate not supplied

[Biological markers in the accurate diagnosis of chronic alcohol intoxication. The significance of gamma-glutamyl transpeptidase].

The late diagnosis of severe alcoholism--often after the onset of somatic complaints and the appearance of psychic disorders needs extensive research able to identify new objective clinical and paraclinical markers to allow early and accurate identification of alcoholism also after various periods of abstinence. The scores obtained are the consequence of alcohol abuse and not of nutritional deficiencies or some other associated visceral-humoral sufferings. The authors underline the significance of gamma-GTP in assessing early severe alcoholism state and the necessity and importance of finding certain specific biological markers--early and sensitive for etillic intoxication--that would considerably simplify the manner of diagnosis and an efficient therapy. The identification of certain significant and specific markers before the onset of visceral-humoral disorders would improve the psychoprotective measures before the onset of tolerance and withdrawal--determinant factors of severe etillic intoxication.

Adult↗
PubMedDate not supplied

[Clinical observations of treatment with Lyorodin-depot (Jenapharm)].

According to the indications of fluphenazin decanoate in the long-term treatment in schizophrenia the authors analyse the findings of a simple blind study of Lyorodin-depot on a sample of 20 inpatients. The drug was administered at intervals of 21 days for 18 weeks under clinical surveillance. The method consisted of patients' follow-up on an itemized card, psychological checkups and para-clinical investigations. The analysis of clinical findings reveals the amelioration of symptoms at the parameters accounted for, the favourable evolution low incidence of the side effects that confirms its clinical efficiency and tolerance.

Adult↗