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V M Man'ko

Publications and source records attributed to V M Man'ko.

At least 19 recordsLinked to original sources
PubMed1996

[B-lymphocyte regulation of the functional activity of hematopoietic stem cells. I. The effect of activated B-lymphocytes from the murine spleen on the proliferation of syngeneic hematopoietic stem cells of the bone marrow].

The hemopoietic stem cells (HSC), obtained from the mouse bone marrow and transferred into lethally irradiated syngeneic mice, do not form colonies of hemopoietic cells in the spleen of recipients. The colony formation has been observed after transplantation of HSC together with bone marrow B-lymphocytes or precursors of T-lymphocytes (PTL), and has been mediated through soluble products of nonimmunoglobulin nature produced by B-cells. In distinction from bone marrow B-cells, the interaction of splenic B-lymphocytes with the HSC fraction does not initiate this process, but suppresses it in the presence of bone marrow PTL. The interaction of B-lymphocytes activated by LPS (in vitro) or sheep erythrocytes (in vivo) with HSC or with a mixture of HSC and PTL resulted in the formation of splenic colonies. However, the induction of colony formation has been observed only in the presence of mature T-lymphocytes (phenotype Thy-1, Sc-1), revealed as an admixture to HSC, and has been abolished after their removing in the process of fractionation of bone marrow and receiving the purified HSC fraction. It requires some membranous interaction of regulatory cells.

Animals↗
PubMed1993

[B-cell immunity].

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Antibody Formation↗
PubMed1992

[The phenotypic characteristics of T-lymphocytes that interact with hematopoietic stem cells: the expression of the antigens Lyt-1, Lyt-2 and L3T4].

The role of T-lymphocytes, bearing antigens Lyt-1, Lyt-2 and L3T4, in regulation of the functional activity of blood-forming precursor cells of syngen and non-syngen origin was investigated. The treatment of cells of murine lymphatic nidi with monoclonal antibodies to the above antigens and with the complement did not abolish the capacity of T-lymphocytes of controlling proliferation and differentiation of syngen and allogen blood-forming precursor cells. The subpopulation characteristics of lymphocytes interacting with the stem cells is discussed.

Animals↗
PubMed1992

[Effects of B-lymphocytes from different organs on hemopoietic colony formation in the spleen by bone marrow cells].

The influence of B-lymphocytes from various sources on splenic colony formation was studied in the syngeneic system. B-lymphocytes were obtained by panning with IgG-fraction of rabbit anti-mouse Ig, absorbed on Petri dishes. In addition, adherent cells, Thy-1+ and SC-1+ were eliminated from the fraction of Ig(+)-cells. SC-1- and SC-1+ fractions, containing, respectively, stem cells and T-lymphocyte precursors, were obtained by panning with IgG-fraction of rabbit anti-SC-1 serum. SC-1- cells transferred to irradiated syngeneic mice did not induce colony formation in the spleen. Introduction of SC-1- and SC-1+ cells induced formation of colonies. A similar helper effect occurred when SC-1(-)-cells were introduced with bone marrow or lymph node B-cells, but not with splenic B-cells. Splenic, but not bone marrow and lymph node B-cells inhibited colony formation by combination of SC-1- and SC-1+ cells. All effects of Ig+ cells were abolished by treatment of cells with rabbit anti-MBLA serum. Thus, B-cells of various origin can either enhance or inhibit colony formation. The enhancing of inhibitory effect after B (MBLA+)-cells elimination from suspension of bone marrow and lymph node (but not spleen) Ig(+)-cells resulted from the activity of B-contrasuppressors.

Animals↗
PubMed1991

[The mitostatic and lymphotoxic action of kemantane and its effect on B-suppressors].

The in vivo study of the influence of Kemantan on the growth of endogenous colonies in the spleen of sublethally (6 Gy) irradiated (CBA x C57BL/6J) F1 mice (mitostatic action) and on the capacity of transplanted lymphocytes of CBA mice for suppressing their multiplication (lymphotoxic action) was carried out. Besides the capacity of Kemantan for affecting the induction, formation and functioning of B-suppressors of antibody formation was studied. As revealed in this study, Kemantan in doses of 0.2-200 mg/kg did not produce a mitostatic and lymphotoxic effect and had no influence on the realization of the suppressing action of mature B-suppressors. In doses of 20 and 200 mr/kg Kemantan, injected to donors at the phase of the induction and accumulation of B-suppressors, abolished their formation.

Adamantane↗
PubMed1991

[The effect of kemantane on T-helpers and T-suppressors].

The study of Kemantan on functionally alternative humoral immunity regulator cells: T-helpers and antigen-specific T-suppressors, including their induction, accumulation and functioning, was studied. Kemantan in doses of 0.2-200 mg/kg, introduced to the donors of T-helpers 2 days before they were taken, stimulated their activity 1.5- to 2-fold (with p less than 0.05). Kemantan had no influence on the functional activity of T-suppressors, as well as on their induction and accumulation.

Adamantane↗
PubMed1990

[Phenotyping of blood and bone marrow lymphocytes in children with primary immunologic deficiency].

Subpopulational composition of peripheral blood and bone marrow lymphocytes was studied in 17 children with different forms of immunodeficiency, combined with hematological shifts (agammaglobulinemia--6, mucocutaneous candidiasis--2, selective IgG-deficiency--2, hyper-IgM syndrome--3, cephalo-oculocutaneous telangiectasia (COCT)--2, general, variable immunodeficiency--2 patients; neutropenia was observed in all the patients, lymphopenia--in 13, anemia-in 6 patients. Surface markers were assayed by flow cytofluorometry with monoclonal antibodies OKT3, OKT4, OKT8, OKB7, produced by "Ortho diagnostics". Changes characteristic of certain forms of primary immunodeficiency have been revealed in the subpopulational composition of peripheral blood and bone marrow lymphocytes: decreased helper potential in patients with general variable immunodeficiency, T-lymphocyte deficiency in patients with COCT increased number of phenotype T3 cells and decreased amount of B-cell in agammaglobulinemia patients. Significant heterogeneity has been noted in the parameters of hemogram, myelogram and in the subpopulational composition of peripheral blood and bone marrow lymphocytes in each nosologic form, the group of patients with hyper-IgM syndrome has proved to be most heterogeneic. It has been suggested that the changes in the subpopulational composition of bone marrow lymphocytes may be responsible for primary immunodeficiency and disorders in hemopoiesis.

Adolescent↗
PubMed1989

[Lymphocyte subpopulations in the bone marrow of children].

Blood lymphocyte subpopulations, and bone marrow aspiration and biopsy materials were comparatively investigated in 10 children without hematologic or infectious diseases (the children had chest deformations and diaphragmatic hernias). Lymphocyte typing was conducted by the method of continuous cytofluorometry in a complete leucocyte suspension with the use of monoclonal antibodies. The results of the study have evidenced that the content of lymphocytes carrying T-cell markers in the aspiration material was twofold lower, and in the biopsy material fourfold lower than in the peripheral blood. The number of immature T-lymphocytes in the bone marrow is higher than their content in the peripheral blood. A total of 40% of aspiration cells and 60% of biopsy cells present in the lymphocytic window, do not carry lymphocytic markers. CD8+-lymphocytes prevail in the bone marrow.

Antibodies, Monoclonal↗
PubMed1989

[Myelopeptide correction of the differentiation of hematopoietic precursor cells in mice with experimental T-immunodeficiency].

The influence of myelopeptides on differentiation of bone marrow haemopoietic precursors cells in thymectomized and normal mice has been studied in vivo. The introduction of myelopeptides decreased the number of erythroid (E) colonies and increased that of granulocytic ones (G). This results in the decrease of initially raised E/G ratio in thymectomized mice (from 4.3) down to 1.3). Myelopeptides exerted no influence on haemopoietic precursors in normal mice (E/G-2.0).

Animals↗
PubMed1988

[Genetic differences of antigens controlled by Y chromosome ensure the inactivation of bone marrow stem cells by syngeneic murine lymphocytes].

Expression of the inactivation effect of the stem colony-forming marrow cells of male mice CBA, C57B1/6, (CBA.C57B1/6)F1 and (C57B1/6.CBA)F1 by syngeneic lymphocytes of lymphatic nodes of females in conditions of transplantation of cell mixtures into lethally irradiated recipients was studied. The maximum inactivation effect of stem cells (86.3%) is determined in mice F1 with differences of H-Y antigen, the minimum effect (2.4%)--in mice CBA, an intermediate effect (54%) being registered in mice C57B1/6. Sensibility of target cells to the inactivation influence of lymphocytes distinguished by weak transplantation H-Y antigen depended on the maternal effect and direction of reaction. The inactivation effect was maximal in the combination CBA----C57B1/6 and C57B1/6----C57B1/6, as compared with the combination CBA----CBA and C57B1/6----CBA. Possible mechanisms by which the killer action of syngeneic lymphocytes on the antigen H-Y of target cells is developed differently, are under discussion.

Animals↗
PubMed1987

[The role of cells sensitive to anti-mouse brain serum in the regulation of CFU-S proliferation].

CFU-S differentiation and regeneration kinetics in the spleen and femur was studied after treatment of bone marrow cells with RAMB serum. The effect of thymocytes on the rate of CFU-S regeneration was also investigated. It was found that CFU-S regeneration in the spleen was similar in RAMBS-treated and intact cell populations on days 4-14 after transplantation. On the contrary, the rate of CFU-S regeneration in the femur was slower in RAMBS-treated than in intact bone marrow cells. However, the growth rate in the femur could be restored to the normal level by the administration of freshly isolated syngeneic thymocytes to mice pre-injected with RAMBS-treated CFU-S population. The treatment of bone marrow suspension with RAMB serum did not affect the differentiation of spleen colonies. It is suggested that RAMBS eliminates cell population regulating CFU-S proliferation, without affecting its differentiation.

Animals↗