Search PubMed⌕ Search

Biomedical subjects

V M Doctor

Publications and source records attributed to V M Doctor.

At least 37 records · Page 2Linked to original sources
PubMed1987

Inhibin-like material--an immunohistologic marker for prostatic origin of metastases.

Immunocytochemical localization of inhibin-like material (ILM) using a specific antiserum generated against ILM of prostatic origin was carried out in metastatic lymph nodes of known primary prostatic tumours and in rectal biopsies showing direct invasion with known prostatic carcinoma. Nine of the 11 metastatic lymph nodes gave positive reaction, which was readily apparent in differentiated tumours showing micro-acinar formation. In poorly differentiated tumours it was often focal and indicated intracytoplasmic staining within randomly scattered cells. Rectal biopsies showing invasion of prostatic carcinoma (4 cases) also showed positive reaction for ILM. Using this experimental protocol, the reaction for metastatic lesions from patients with non-prostatic carcinomas was completely negative indicating the specificity of the test for prostatic origin of metastasis. In conclusion, the present investigation indicates the potential application of ILM in confirming or excluding the prostatic origin of invasive tumour in metastatic lesions.

Humans↗
PubMed1986

Clinico-pathological study of fallopian tubes after transcervical insertion of quinacrine hydrochloride pellets.

This study lends support to others indicating the apparent safety and effectiveness of multiple transcervical insertions of quinacrine hydrochloride as pellets in 240 mg dosage to achieve permanent sterilization. In order to study the effects of the number of quinacrine pellet insertions and the site of placement of the pellets in the uterus of prehysterectomy volunteers, a scoring system of histological changes in the Fallopian tube was designed. Quinacrine pellets were deposited at the fundus using a straight inserter in 16 women, and at the cornua using a curved inserter in 17 women. Each group had at least five women receiving one, two or three insertions at one-week intervals. Results indicate that neither the number of insertions nor the place of deposition of the pellets affects the degree of tubal inflammation and fibrosis.

Adult↗
PubMed1986

Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: comparison of its distribution in normal, benign and malignant prostates.

A specific antiserum has been generated against inhibin-like material (ILM) of prostatic origin. Using the immunoperoxidase technique, localization of ILM has been examined in a total of 114 prostates including normal (4 specimens), malignant (46) and hyperplastic (55) tissues. ILM positive immunocytochemical reactions were confined to the cytoplasm and not the nucleus of the prostatic acinar cells in the three categories of prostate, whereas the stroma showed negative reactions. The intensity of positive reactions decreased in the following order: Hyperplasia, incidental and moderately differentiated carcinomas, poorly differentiated carcinomas, whereas metaplasia and granulomatous prostatitis gave negative reactions for ILM. Using this experimental protocol, 200 non-prostatic tissue were found to be completely negative, demonstrating the specificity of the test for prostatic epithelium. These findings indicate a potential use of ILM as a marker of prostatic tissue.

Humans↗
PubMed1985

Cellular immunolocalization of inhibin-like peptide in human benign prostatic hyperplasia.

Immunoperoxidase staining method and specific antisera against highly purified inhibin-like material (ILM) from human seminal plasma were utilized to localize immunoreactive ILM in benign hyperplastic prostate (BPH) in human. Immunoreactive ILM was strictly localized to glands of hyperplastic nodules. The concentration of ILM was more in epithelial cells lining the healthy lobule than in those undergoing atrophy.

Aged↗
PubMed1983

Isolation and anticoagulant properties of polysaccharides of Typha Augustata and Daemonorops species.

Polysaccharides were isolated from Dragon's Blood (DB), a red resinous secretion from the fruits of Daemonorops species and from pollens of Typha Augustata (TA) by alcohol precipitation followed by deaminoethylaminoethyl (DEAE) cellulose chromatography. The purified polysaccharides of DB and TA gave specific rotations of [alpha]D20 64 and [alpha]D20 of 80 and their molecular weights by membrane osmometry were approximately 25000 and approximately 30000 respectively. The effect of addition of the polysaccharides on coagulation of pooled normal human plasma was investigated. They were found to accelerate the recalcified plasma times at concentrations below 100 micrograms/ml while inhibiting them at higher concentrations. Their procoagulant properties were due to their effects on the activation of Hageman factor (XII) while the anticoagulant effect was mainly directed towards fibrinogen. Studies of the mechanism of the anticoagulant effect showed that addition of TA polysaccharide inhibited the rate of release of fibrinopeptides by thrombin and also the aggregation of fibrin monomers.

Anticoagulants↗
PubMed1983

Isolation and properties of a new anticoagulant protein from commercial bovine testicular hyaluronidase.

Bovine testicular hyaluronidase from various commercial sources showed the presence of an inhibitor of human plasma prothrombin time (PT). A testicular anticoagulant protein (TAP) was isolated from it by a 3-step procedure. The material was first passed through conconavalin-A-sepharose affinity chromatography where the anticoagulant material was separated from the hyaluronidase and protease which were retained by the column. In the second step the lower molecular weight proteins were removed by ultrafiltration. The supernatant which contained the anticoagulant protein was passed through the carboxymethyl cellulose column and the active material was eluted by 0.4 M NaCl solution. Sodium dodecyl sulfate (SDS) gel electrophoresis gave a molecular weight of approximately 35000. Unlike many small molecular weight proteins from bovine testes, TAP looses its anticoagulant property by heating for 30 minutes at 55 degrees C or by storage at pH 3.0 for 2 hours and it does not inhibit trypsin or thrombin. Its isoelectric pH was 9.7. Diisopropyl fluorophosphate (DFP) treated TAP was not effective as an anticoagulant.

Animals↗
PubMed1983

Isolation and anticoagulant properties of a new sulfated xylan: comparison with heparin and a sodium pentosan polysulfate (SP-54).

Larchwood xylan was purified by diaminoethylaminoethyl (DEAE) cellulose chromatography, sulfated by heating with chlorosulfonic acid -pyridine complex and the sulfated polysaccharide was isolated by epichlorohydrin triethanolamine (ECTEOLA) cellulose chromatography as the sodium salt. It's molecular weight was approximately 25000 and the specific rotation was [alpha]D 20-70 degrees. Electrophoretic analysis of the sulfated xylan along with heparin and SP-54 using lithium acetate-agarose technique showed that the charge density of the xylan sulfate was similar to heparin and it moved as a single component in contrast to commercial heparin which resolved into two while SP-54 moved at the same rate as the marker dye. Anticoagulant properties of the sulfated xylan were compared with heparin and SP-54 by studying its effect on the activated partial thromboplastin time (APTT), prothrombin time (PT) and the thrombin time (TT) using pooled normal human plasma. The sulfated xylan was more effective than SP-54 in delaying coagulation by all the three measurements while it was less active than heparin in inhibiting APTT and PT.

Anticoagulants↗
PubMed1975

Distribution of fatty acids during germination of cottonseeds.

Gas chromatographic determination of the fatty acids in the seeds of cottonseed (Gossypium sp.) showed linoleic, palmitic, oleic, and stearic acids, with linoleic acid being the major component. Changes in the composition of fatty acids during various stages of germination were measured in the cotyledons and in the roots. A decrease in the content of all the fatty acids and an 8-fold increase in the moisture content of the cotyledons were observed during the 10 days of germination. There were no significant changes in the fatty acid contents of the roots with the exception of those in linoleic acid which increased by 50 percent during 4-10 days of germination. The possible significance of these changes is discussed.

Cell Division↗
PubMed1975

Separation of (3') deoxynucleotides with cation exchange columns.

A procedure is described for a one-step separation of synthetic (3') deoxynucleotides purchased commercially or those purified and isolated from the enzymatic digests of DNA. The method is simple. A 50 ml buret was used for column which was filled with the resin slurry and packed at water aspirator pressure. The compounds were eluted from the column at atmospheric pressure under gravity flow using a fraction collector and read on a Beckman DU spectrophotometer. Recoveries were in excess of 90% and no accessory devices were required. The use of a volatile buffer, e.g. ammonium formate facilitated the recovery of the purified material by allowing the evaporation of the medium in which the sample was eluted.

Cation Exchange Resins↗