[Role of Chlamydia and Mycoplasma genital infections in salpingitis and tubal sterility].
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Biomedical subjects
Publications and source records attributed to V Loffredo.
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We have greatly improved in prevention of adhesions after tuboplasty, using two different and new technics: --peritoneal grafts on crude surfaces (21 cases) --laparoscopy 8 days after tuboplasty (54 cases), and sweeping off fresh adhesions with a rod. First results seem to confirm the interest of these methods.
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Research was made for chlamydia trachomatis and ureaplasma urealyticum in the peritoneum and the tubes of 99 women divided into 4 groups: 17 of them were being investigated because of acute salpingitis (Group A), 17 were being investigated for tubal sterility with chronic inflammation diagnosed laparoscopically (Group B), 29 were being investigated for tubal sterility without any laparoscopic evidence of inflammation (Group C) and 36 women had absolutely normal pelves and were being investigated for sterility. These were the control group (D). Swabs were also taken from the lower genital tracts as well as serological tests for chlamydia trachomatis and cytological samplings of the fluid from the Pouch of Douglas and the histology of the tubes. In the 17 women who had acute salpingitis the swabs 4 cases of C.T. and 4 of U.U. In the 46 women who had tubal sterility the laparoscopic swabs showed cases of C.T. and 7 of U.U. The swabs were most often positive in Group B. This group is characterised by a special appearence of the inflammation, with fluid present and viscous adhesions as well as peritoneal inflammatory cysts. These altogether help to make a presumptive diagnosis of C.T. infection on laparoscopy. In the control group of 36 cases there was no sign of C.T. in any case, although 2 swabs from the peritoneum showed U.U. So there is a statistically significant difference between the groups that were suspicious and the control group whether the results were obtained by cultures or by serological diagnosis. On the other hand there is no definitive difference as far as U.U. is concerned. These observations, which are similar to those published by other authors, lead us to think that micro-organisms and especially chlamydia trachomatis could be the bacteriological agent responsible for chronic inflammatory states found so frequently in women with tubal sterility.
Out of a series of 50 salpingitis, we have made bacteriological swabs of tubes and peritoneum in 37 cases (25 cases by laparoscopy and 2 cases by laparotomy). 27 women had received no antibiotic treatment before swabbing: bacteriological culture was positive in 14 cases and gram Stain positive in 15 cases: thus we had either a complete study, either a "good idea" of pathological flora in 20 cases out of 27. 10 women received some antibiotic treatment before swabbing: bacteriological culture was positive in 2 cases, gram stain positive in 3 cases. We had idea of pathological flora in 4 cases out of 10. Tubal cultures show either a one-agent infection (gonococcus, E. Coli or anaerobic agent) either a various aero-anaerobic flora. In 18 cases we could compare abdominal flora and low genital tract flora: results were identical in half of cases only (7 cases on 18). Gonococcus was cultured either in women with P.I.D. either in their partner's genital tract, in 11 cases on 37. Both cultures and gram stain were negatives in 13 cases: in these cases, we could perhaps incriminate either a supplement requiring bacterie, either other micro-organisms (chlamydia trachomatis or Mycoplasma) which will be studied in a further series (to be published).
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We have greatly improved in prevention of adhesions after tuboplasty, using peritoneal grafts on crude surfaces (28 cases off 92 tuboplasties). First results seem to confirm the interest of this method.
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Laparoscopy was performed 8 days after tuboplasty in 80 cases. In 32 cases, new fresh adhesions were swept out with a rod on the 8th day. These adhesions were important in 13 cases (16,2 p. 100). This treatment seems to be efficient.
We report six case histories of malignant tumours following abortion of moles. In three cases their surgical removal allowed us to control them by histology: two of these were destruens chorio-adenomata, one case only was a choriocarcinoma. In the three other cases the cure was obtained by the sole use of anti-mitotic drugs. This treatment was undertaken on bioclinical evidence of malignancy which is certainly not foolproof, but which is practical in use, when one appreciates how difficult it is to diagnose some of these post-mole tumours histologically. There is a place for the surgical treatment of these cases in spite of the usefulness of Methotrexate. This place varies according to the age of the patients and the resistance of the tumours to treatment with anti-mitotic drugs.