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Biomedical subjects

V Liebers

Publications and source records attributed to V Liebers.

23 records · Page 2Linked to original sources
PubMed1992

Insect hemoglobins (Chi tI) of the diptera family Chironomidae are relevant environmental, occupational, and hobby-related allergens.

Six hundred and forty-two persons with hobby-related (n = 205), occupational (n = 85), or environmental contact (n = 352) to the midge and larval allergen Chi tI were studied. Frequencies of IgE-mediated sensitization in these selected populations were 36.1%, 24.7%, and 9.6% respectively. Occupationally sensitized subjects who had been heavily exposed showed higher levels of antibodies, were more frequently diagnosed as having bronchial asthma and less frequently as having conjunctivitis, and had a significantly shorter latency period when compared to environmentally exposed people or aquarists. Our results are evidence for a higher risk of sensitization and of bronchial asthma in highly exposed subjects.

Allergens↗
PubMed1991

[Aquarium--when hobby becomes a danger. A case report].

In this case report, allergic rhinitis and episodes of asthma triggered by the hemoglobin of the red mosquito larva used as fish food are described. After three years of contact and an allergic condition persisting for three months, skin testing and IgE determination revealed moderate sensitization of the immediate reaction type. When contact with the allergen was stopped, the symptoms abated immediately.

Adult↗
PubMed1991

Immunological cross-reactivity of hemoglobins in the Diptera family Chironomidae.

Polyclonal human IgE antibodies of patients sensitized to one species of the Diptera family Chironomidae as well as polyclonal rabbit anti-Chi t I hyperimmune serum recognize antigenic sites present in nearly all of the 33 species of this insect family. Evolutionary distantly related genera usually show weaker antibody binding. According to the pattern of reactivity of rabbit anti-Chi t I-component III, expression of the epitopes of this molecule varies considerably in the genera Chironomus and Glyptotendipes; it appears to be almost totally absent in all other species. Of five monoclonal antibodies raised against Chi t I-component III, two recognize an epitope which is expressed in nearly all closely related species. Three monoclonal antibodies recognize epitopes which are expressed in only a few species of the same genus. Our results demonstrate the presence of common as well as species-specific epitopes in chironomid hemoglobins which behave as potent inhalant allergens.

Animals↗
PubMed1991

Stimulation of human peripheral blood lymphocytes with chironomid hemoglobin allergen (Chi t I).

Hemoglobins (Chi t I) of the dipteron species Chironomus thummi thummi are known to cause severe allergic diseases in humans. We tested the allergen-specific stimulation of human peripheral blood lymphocytes (PBL) by Chi t I and its nine main components. Further, we applied fragments of the well-analyzed component III, obtained by cleavage with trypsin as well as arginine protease. In this way, we screened the molecule in order to identify T-cell epitopes. The whole component was found to be immunogenic and to have regions demonstrating varying PBL stimulation. In addition, interindividual patterns of reactivity, probably due to genetic restriction, were found. A T-cell epitope could be shown to be within the site 98-111, as predicted by application of Rothbard's algorithms.

Allergens↗