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Biomedical subjects

V L Weimar

Publications and source records attributed to V L Weimar.

15 recordsLinked to original sources
PubMed1982

Synergistic growth stimulation of corneal fibroblasts by components of mesodermal growth factor from murine submaxillary glands.

Recycled mesodermal growth factor (R-MGF) is a pool of proteins of 26,000 molecular weight obtained by recycling gel chromatography of male murine submaxillary gland extracts. R-MGF strikingly accelerates corneal stromal wound healing in vivo, fibroblast growth and migration in cultured corneal buttons and is showing here to stimulate stromal fibroblast growth and division in tissue culture. Chromatographic fractionation of R-MGF has yielded several components, none of which has a greater biological potency than the parent R-MGF. In contrast, two components, MGF-I and -II, when recombined synergistically stimulate fibroblast response in tissue culture and organ culture in excess of those obtained with the parent R-MGF. Three MGF components (I, III, and IV) have been purified and are inactive at 10-15 micrograms/ml in organ culture but potently stimulate fibroblast responses when combined in pairs containing 7.5 micrograms of each component. The striking synergism in organ culture suggests that the stimulation of wound healing by R-MGF in vivo may also reflect synergistic action of more than one R-MGF component. Procedures for isolating gram quantities of R-MGF and for the purification of different R-MGF components by ion exchange chromatography are detailed.

Animals↗
PubMed1980

Acceleration of healing of rabbit corneal endothelium by mesodermal growth factor.

Freeze-thaw injuries of rabbit corneas were treated with mesodermal growth factor (MGF) in organ culture and the repair of the endothelium followed by light microscopic examination of endothelial whole mounts. MGF-treated corneas showed an increased number of mitotic figures. In addition, MGF accelerated the rate of cell hypertrophy, formation of spindle-shaped cells, and the rate of their migration into the killed zone so that Descemet's was covered more rapidly than was that of the controls. In MGF-treated corneas, the cells lost their fibroblast-like appearance and became endothelial-like in appearance sooner than did the untreated corneas. A short period of treatment (6 hr) was sufficient to stimulate the repair processes described above. The healing responses obtained in organ culture for control eyes approximately those described in the literature for freeze-thaw injuries to the rabbit endothelium in vivo. The data presented also show that it is possible to accelerate endothelial regeneration in wound healing.

Animals↗
PubMed1979

Stimulation of corneal wound healing with mesodermal growth factor.

Mesodermal growth factor (MGF) from mouse submaxillary glands was tested in vivo for stimulating effects on corneal wounds in rabbits. Intrastromal injection of 5 microgram of MGF induced widespread fibroblast activity and stromal cell division, and markedly stimulated stromal healing. At high doses (greater than 25 microgram), corneal destruction was indicated by extensive necrosis and perforation. When low doses (1 to 5 microgram) of MGF were applied to the lip of nonperforating knife wounds of the cornea, three major differences were noted between control and experimental wounds. In wounds treated with MGF, the depth of stromal healing was greater, as was the intensity of the fibroblast activity, and the width and depth of the epithelial plug were significantly decreased. These results establish that MGF is an effective growth-stimulating agent in vivo and that the initial stages of corneal wound healing may be accelerated in vivo.

Animals↗
PubMed1979

Activation of initial wound healing responses in rat corneas in organ culture by mesodermal growth factor.

In organ culture, mesodermal growth factor (MGF) from mouse submaxillary glands stimulates fibroblast formation, mitosis, and migration of cells into the dead-cell zone adjacent to the wound edge of rat corneal buttons. A classic dose-response relationship between the width of the stromal fibroblast zone and the dose of MGF suggests that fibroblast zone width may be used as a measure for screening agents which influence the initial stages of corneal wound healing.

Animals↗
PubMed1975

A potent new mesodermal growth factor from mouse submaxillary gland. A quantitative, comparative study with previously described submaxillary gland growth factors.

A new growth factor with potent growth stimulating effects on connective tissue cells of the cornea has been isolated and partially purified from mouse submaxillary glands. A computerized image analysis system has been used to quantitate the growth responses of connective tissue fibroblasts to this new growth factor and to compare its activity with that of four previously described growth factors [nerve growth factor (NGF), epidermal growth factor (EGF), mesodermal growth factor (MGF), and thymocyte-transforming factor (TTF)] also obtained from mouse submaxillary glands. The new factor is as potent a growth stimulator as the previously described MGF. All five of the growth factors possessed esteropeptidase activity (trypsin-like) and general proteolytic activity. There was no correlation between the degree of growth stimulation of the connective tissue cells and either the esteropeptidase or general proteolytic activities of any of the growth factors.

Animals↗