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Biomedical subjects

V Kruse

Publications and source records attributed to V Kruse.

24 records · Page 2Linked to original sources
PubMed1976

Production and evaluation of high-quality thyroxine antisera for use in radioimmunoassay.

High-quality thyroxine (T4) antisera were raised in rabbits. Conjugates of protein and T4 or T4 methyl ester were given intracutaneously in Freund's complete adjuvant at one or two sites on the back at 1/2- to 3-month intervals. Eleven of 16 immunized rabbits produced antiserum with titers higher than 2 commercial antisera and 4 antisera from other laboratories. All 16 antisera had equilibrium constants (K) higher than the 6 reference sera. The highest titer observed was 130,000 with 88 fmol labeled T4 per ml incubate. A drastic increase in K from about 4 X 10(8) to 5.8 X 10(10) 1/mol was observed as the immunization proceeded. Cross-reactivity with triiodothyronine (T3) varied but was generally low. Suppression of the T4 synthesis was attempted by giving 30 nmol (20 mug) T3 per os once a day. Antiserum from rabbits given T3 suppression contained less iodine than antiserum from nonsuppressed control rabbits. The possible reasons for the successful production of the high-quality antisera are discussed.

Animals↗
PubMed1975

[Intravenous cholegraphy and diaphragmatic movements in the differential diagnosis of acute cholecystitis (author's transl)].

Intravenous cholegraphy was performed in 79 patients with abdominal symptoms suggestive of acute cholecystitis. Three excretion patterns were found: 1. Opacification of the gall bladder and common bile duct. 2. Opacification of the common bile duct. 3. No opacification of the gall bladder or common bile duct. 29% showed opacification of the gall bladder and common bile duct; none of these had acute cholecystitis. 51% of cases showed opacification of the common bile duct only; 82.5% of these had acute cholecystitis. 79.4% of patients with this excretion pattern were shown to have acute cholecystitis at operation. In the other cases, absence of opacification of the gall bladder was due to obstruction of the cystic duct, caused either by concretions or fibrosis. The biliary tree was not visible in 21% of cases. The most common diagnosis was acute cholecystitis, but this excretion pattern is of limited value in differential diagnosis. The value of this examination is stressed, but it must be carried on for an adequate period. Mobility of the right diaphragm was examined preoperatively in 34 patients. Compared with patients with other diagnoses, there was a significant number of patients with acute cholecystitis who showed limited movement. The examination may be regarded as supplementary to intravenous cholegraphy in the diagnosis of acute cholecystitis.

Abdomen, Acute↗
PubMedDate not supplied

Characterization of monoclonal antibodies against bovine insulin.

Six different monoclonal antibodies (IgG1 and IgG2a) were obtained after fusions of X63-Ag8-6.5.3 myeloma cells with spleen cells from BALB/c mice immunized with bovine insulin. Definition of binding determinants was attempted by competitive binding studies with insulins, proinsulins and modified insulins from various species. The monoclonal antibodies OXI-001 and OXI-004 were inferred to react with a region including residue A10, OXI-002 with an antigenic determinant in the B26-30 region, OXI-005 with a region including B30 and OXI-006 with a tertiary structure near the N-terminus of the B chain, possibly including B3 and A10. The equilibrium binding constants for these antibodies were calculated by three different methods (Scatchard, Langmuir and non-linear regression) and were found to be in the range of 2 X 10(7)-8 X 10(9), with good agreement between the different methods of calculation. As expected for a given monoclonal antibody, the heterogeneity index was close to 1.0, as calculated from Sip's logarithmic transformation of the binding equation. These parameters were compared to those of a mixture of the six different monoclonal antibodies and those of a conventional hyperimmune anti-insulin serum (guinea-pig). The half-dissociation times (t1/2) of complexes of antibody and bovine insulin ranged from 35 min to 38 h.

Amino Acid Sequence↗