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Biomedical subjects

V K Garg

Publications and source records attributed to V K Garg.

At least 19 recordsLinked to original sources

Development of a water hyacinth based vermireactor using an epigeic earthworm Eisenia foetida.

The aim of this work was to investigate the potential of water hyacinth (WH) spiked with cow dung (CD) into vermicompost. Five vermireactors containing WH and CD in different ratios, were run under laboratory conditions for 147 days. The maximum worm growth was recorded in CD alone. Worms grew and reproduced favourably in 25% WH+75% CD feed mixture. Greater proportion of WH in feed mixture significantly affected the biomass gain, hatchling numbers and numbers of cocoons produced during experiments. In all the vermireactors, there was significant decrease in pH, TOC and C:N ratio, but increase in TKN, TK and TAP at the end. The heavy metals content in the vermicomposts was lower than initial feed mixtures. The results indicated that WH could be potentially useful as raw substrate in vermicomposting if mixed with up to 25% in cow dung (on dry weight basis).

Animals↗

Fluoride in drinking water and human urine in Southern Haryana, India.

The objective of this study was to determine the fluoride content in drinking water and urine samples of adolescent males aged 11-16 years living in Southern Haryana, India. A total of 30 drinking water sources in the studied habitations were assessed for fluoride contamination. Fluoride was estimated in the urine of 400 male children randomly selected from these habitations. The fluoride concentration in drinking water and urine samples was determined using USEPA fluoride ion selective electrode method. The mean fluoride concentration in drinking water samples of Pataudi, Haily Mandi and Harsaru villages was 1.68+/-0.35, 3.22+/-1.18 and 1.78+/-0.12 mg/l, respectively. The mean urinary fluoride concentration was 2.26+/-0.024 mg/l at Pataudi, 2.48+/-0.77 mg/l at Haily Mandi and 2.43+/-0.84 mg/l at Harsaru village. The higher fluoride levels in the urine of children may be associated to higher fluoride levels in drinking water. The accuracy of measurements was assessed with known addition method in water and urine. Mean fluoride recovery was 98.0 and 99.1% in water and urine. The levels obtained were reproducible with in +/-3% error limit.

Adolescent↗

Removal of hexavalent chromium from aqueous solution by agricultural waste biomass.

In the present study adsorption of Cr(VI) from aqueous solutions onto different agricultural wastes, viz., sugarcane bagasse, maize corn cob and Jatropha oil cake under various experimental conditions has been studied. Effects of adsorbent dosage, Cr(VI) concentration, pH and contact time on the adsorption of hexavalent chromium were investigated. The concentration of chromium in the test solution was determined spectrophotometrically. FT-IR spectra of the adsorbents (before use and after exhaustion) were recorded to explore number and position of the functional groups available for the binding of chromium ions on to studied adsorbents. SEMs of the adsorbents were recorded to explore the morphology of the studied adsorbents. Maximum adsorption was observed in the acidic medium at pH 2 with a contact time of 60min at 250rpm stirring speed. Jatropha oil cake had better adsorption capacity than sugarcane bagasse and maize corn cob under identical experimental conditions. The applicability of the Langmuir and Freundlich adsorption isotherms was tested. The results showed that studied adsorbents can be an attractive low cost alternative for the treatment of wastewaters in batched or stirred mode reactors containing lower concentrations of chromium.

Adsorption↗

Vermiconversion of wastewater sludge from textile mill mixed with anaerobically digested biogas plant slurry employing Eisenia foetida.

Vermicomposting is commonly used for the management of organic wastes. We have investigated the potential of an epigeic earthworm, Eisenia foetida, to transform solid textile mill sludge (STMS) spiked with anaerobically digested biogas plant slurry (BPS) into vermicompost to evaluate the feasibility of vermicomposting in industries for waste management. The growth and reproduction of E. foetida was monitored in a range of different feed mixtures for 15 weeks in laboratory under controlled experimental conditions. E. foetida did not survive in fresh STMS. But worms grew and reproduced in STMS spiked with BPS feed mixtures. A greater percentage of STMS in feed mixture affected biomass gain and cocoon production by earthworms. The maximum growth was recorded in 100% BPS. The net weight gain by E. foetida in 100% BPS was two-four-fold higher than STMS-containing feed mixtures. After 15 weeks, maximum cocoons (78) were counted in 100% BPS and minimum (26) in 60% BPS+40% STMS feed. Vermicomposting resulted in pH shift toward acidic, significant reduction in C:N ratio, and increase in nitrogen, phosphorus, and potassium contents. Microbial activity measured as dehydrogenase activity increased with time up to day 75 but decreased on day 90, indicating the exhaustion of feed and decrease in microbial activity. These experiments demonstrate that vermicomposting can be an alternate technology for the recycling and environmentally safe disposal/management of textile mill sludge using an epigeic earthworm, E. foetida, if mixed with anaerobically digested BPS in appropriate ratios.

Animals↗

A pilot scale evaluation for adsorptive removal of lead (II) using treated granular activated carbon.

Wastewaters discharged from the defence serviceable industries pose a serious environmental hazard due to their heavy metal load. The present study focused on optimizing the operational variables viz, hydraulic loading rate, bed height and feed concentration through bench scale study and using that for assessing the efficiency of pilot scale system with sulphur loaded carbon (AC-S) as the adsorbent in the removal of Pb (II). Static mode adsorption studies were also carried out for Pb (II) removal using treated (AC-S) and untreated carbon (AC). AC-S shows about 35 percent increase in maximum adsorption capacity over that on AC. The maximum adsorption capacity in the column mode for Pb (II) at the optimized conditions: bed height of 0.4 m, hydraulic loading rate of 7.5 m3h(-1)m(-2) and the feed concentration of 6 mg l(-1) for achieving 50% breakthrough concentration was found to be 2.89 mg g(-1). Adsorption mechanism involved during Pb (1) in the column has also been explored. Bohart-Adams model was used for modeling the bench scale data and predicting the adsorption behavior at pilot scale level.

Adsorption↗

Exchangeable sodium induced changes in yield, water relation and cation composition of fennel (Foeniculum vulgare Mill).

A pot experiment was conducted with the objectives to assess the adaptation potential of fennel crop grown at 10, 20, 25, 35 and 40 ESP (exchangeable sodium percentage) levels. Results showed that the rate of seed germination, plant growth including branching pattern, umbels per plant and 1000 test seed weight were adversely affected by sodic soils. Assuming that fifty percent reduction in seed yield and Na+/K+ ratio in leaf tissue as an index of alkali tolerance revealed that fennel was tolerant up to 25 ESP. The cell sap pH and EC reflected optimum osmoticum maintenance to withstand sodicity stress at this level and beyond this leaf water potential decreased (negatively) more to impede water uptake.

Biomass↗

Effect of textile effluents on growth performance of wheat cultivars.

Laboratory experiments were conducted to study the effect of different concentrations in the range of 0-100% textile effluents (untreated and treated) on seed germination (%), delay index (DI), plant shoot length and root length, plant biomass, chlorophyll content and carotenoid of three different cultivars of wheat. The textile effluent did not show any inhibitory effect on seed germination at low concentration (6.25%). The other reported plant parameters also followed the similar trend. Seeds germinated in undiluted effluents did not survive for longer period. Based on the tolerance to textile effluent, the wheat cultivars have been arranged in the following order: PBW-343 < PBW-373 < WH-147. It has also been concluded that effect of the textile effluent is cultivar specific and due care should be taken before using the textile effluent for irrigation purpose.

Biomass↗

Vermistabilization of textile mill sludge spiked with poultry droppings by an epigeic earthworm Eisenia foetida.

Investigations were made to explore the potential of an epigeic earthworm Eisenia foetida to transform textile mill sludge spiked with poultry droppings in to value added product, i.e., vermicompost. The growth and reproduction of E. foetida was monitored in a range of different feed mixtures for 77 days in the laboratory under controlled experimental conditions. The maximum growth was recorded in 100% cow dung (CD). Replacement of poultry droppings by cow dung in feed mixtures and vice versa had little or no effect on worm growth rate and reproduction potential. Worms grew and reproduced favourably in 70% poultry droppings (PD)+30% solid textile mill sludge (STMS) and 60% PD+40% STMS feed mixtures. Greater percentage of STMS in the feed mixture significantly affected the biomass gain and cocoon production. Net weight gain by earthworms in 100% CD was 2.9-18.2 fold higher than different STMS containing feed mixtures. The mean number of cocoon production was between 23.4+/-4.65 (in 100% CD) and 3.6+/-1.04 (in 50% PD+50% STMS) cocoons earthworm(-1) for different feed mixtures tested. Vermicomposting resulted in significant reduction in C:N ratio and increase in nitrogen and phosphorus contents. Total potassium, total calcium and heavy metals (Fe, Zn, Pb and Cd) contents were lower in the final product than initial feed mixtures. Our trials demonstrated vermicomposting as an alternate technology for the recycling and environmentally safe disposal/management of textile mill sludge using an epigeic earthworm E. foetida if mixed with poultry droppings.

Animals↗

Groundwater quality in some villages of Haryana, India: focus on fluoride and fluorosis.

The fluoride concentration in underground water was determined in four villages of Jind district of Haryana state (India) where it is the only source of drinking water. Various other water quality parameters such as pH, electrical conductivity, total dissolved salts, total hardness, total alkalinity as well as sodium, potassium, calcium, magnesium, carbonate, bicarbonate, chloride and sulfate concentrations were also measured. A systematic calculation of correlation coefficients among different physico-chemical parameters was performed. The analytical results indicated considerable variations among the analyzed samples with respect to their chemical composition. Majority of the samples do not comply with Indian as well as WHO standards for most of the water quality parameters measured. The fluoride concentration in the underground water of these villages varied from 0.3 to 6.9 mg/l, causing dental fluorosis among people especially children of these villages. Overall water quality was found unsatisfactory for drinking purposes without any prior treatment except at eight locations out of 60.

Alkalies↗

Adsorption of chromium from aqueous solution on treated sawdust.

The adsorption of Cr(VI) from aqueous solutions on formaldehyde treated sawdust (SD) and sulphuric acid treated sawdust carbon (SDC) of Indian Rosewood, a timber industry waste, was studied at varying Cr(VI) concentrations, adsorbent dose, pH and agitation time. Similar experiments were conducted with commercially available coconut based activated carbon to compare the results. The Cr(VI) adsorption efficiency on SDC was higher than SD. The adsorption followed first order rate expression and Lagergren equation. An initial pH of 3.0 was most favorable for Cr(VI) removal by both the adsorbents. Maximum Cr(VI) was sequestered from the solution within 60 min after the beginning for every experiment. It is proposed that SDC and SD can be potential adsorbents for Cr(VI) removal from dilute solutions.

Adsorption↗

Dynamics of biological and chemical parameters during vermicomposting of solid textile mill sludge mixed with cow dung and agricultural residues.

In India, thousands of tons of textile mill sludge are produced every year. We studied the ability of epigeic earthworm Eisenia foetida to transform textile mill sludge mixed with cow dung and/or agricultural residues into value added product, i.e., vermicompost. The growth, maturation, mortality, cocoon production, hatching success and the number of hatchlings were monitored in a range of different feed mixtures for 11 weeks in the laboratory under controlled environmental conditions. The maximum growth and reproduction was obtained in 100% cow dung, but worms grew and reproduced favorably in 80% cow dung + 20% solid textile mill sludge and 70% cow dung + 30% solid textile mill sludge also. Addition of agricultural residues had adverse effects on growth and reproduction of worms. Vermicomposting resulted in significant reduction in C:N ratio and increase in TKN, TP, TK and TCa after 77 days of worm activity in all the feeds. Vermicomposting can be an alternate technology for the management of textile mill sludge if mixed with cow dung in appropriate quantities.

Animals↗

Ground water contamination in the area adjoining zinc smelter effluent stream.

A preliminary survey of the area adjoining to zinc smelter effluent stream was undertaken to assess ground water contamination. Twenty-five ground water samples from the wells located in the study area were collected and grouped into nine groups based on their lateral and longitudinal position from the stream carrying the effluent discharged from zinc smelter, Debari- Udaipur. The study indicate that waters of wells situated in the vicinity of effluent stream (255m radius) were of medium salinity having higher levels of Ca and Mg and lower values of pH as compared to the waters of the reference wells situated far away from the effluent stream. The Fe, Zn, Cd, Cl, F and SO4 contents ofthe water from the wells located within 80m vicinity of the effluent stream were above the permissible limits for drinking purposes. Furthermore the Zn, Cl and SO4 levels in these waters were so high that they were even not suitable for irrigation, indicating a gradual encroachment of effluent into the native ground water.

Bicarbonates↗

Dye removal from aqueous solution by adsorption on treated sawdust.

Formaldehyde treated and sulphuric acid treated saw dusts were used to adsorb malachite green at varying dye concentration, adsorbent dose, pH and agitation time. Similar experiments were conducted with laboratory grade activated carbon to compare the results. The adsorption efficiency of sulphuric acid treated sawdust (SD) was higher than formaldehyde treated SD. The adsorption followed first order rate expression and Lagergren equation. An initial pH in the range of 6-9 was favorable for the dye removal by both the adsorbents. Dilute solutions were effectively decolorized by the adsorbents. It is proposed that in batch or stirred tank reactors, both adsorbents can be an attractive option for dye adsorption.

Adsorption↗

Vermicomposting of mixed solid textile mill sludge and cow dung with the epigeic earthworm Eisenia foetida.

This paper reports the results of a study of vermicomposting with Eisenia foetida of solid textile mill sludge mixed with cow dung in different ratios in a 90 days composting experiment. Vermicomposting resulted in significant reduction in C:N ratio and increase in TKN. Total K and Ca were lower in the final cast than the initial feed mixture. Microbial activity measured as dehydrogenase activity increased up to 75 days and decreased on further incubation. Total P was higher in the final product than the initial feed mixture. Total heavy metal contents were lower in the final product than initial feed mixture. Solid textile mill sludge can be potentially useful as raw substrate in vermicomposting if mixed with up to 30% cow dung (on dry weight basis). The growth and cocoon production of the worm species in different feed mixtures were also investigated.

Animals↗

Post-kala-azar dermal leishmaniasis in Nepal.

BACKGROUND: Post-kala-azar dermal leishmaniasis (PKDL) manifests as a skin eruption after healing of visceral leishmaniasis (VL), either spontaneously or as a result of treatment. This study was undertaken to describe the demographic, clinical, and histopathologic features of PKDL in Nepal. METHODS: Demographic, clinical, microbiologic, and histopathologic features and response to treatment were studied in 22 patients with PKDL from April 1998 to March 2000. RESULTS: PKDL accounted for 0.13% of all new dermatologic cases. There were 13 (59.1%) males and nine (40.9%) females. A past history of kala-azar was present in all but one patient. A family history of kala-azar was noted in eight (36.4%) patients. All patients presented with multiple types of lesion, except for two in whom only macular lesions were seen. Oral lesions in the form of nodules and plaques were seen in four patients. Generalized lymphadenopathy was present in five patients. Slit skin smears revealed Leishman-Donovan bodies (LDBs) in nine (40.9%) patients. In macular lesions, there was a sparse infiltrate of plasma cells, lymphocytes, or histiocytes in the upper dermis. There was a dense chronic inflammatory infiltrate comprising plasma cells, lymphocytes, histiocytes, and epithelioid cells in the entire dermis from papules, plaques, or nodules. Giemsa staining of biopsy specimens revealed LDBs in seven (38.9%) patients only. Fine needle aspiration from epitrochlear lymph nodes in two patients demonstrated LDBs. All patients responded well to treatment with minimal side-effects. CONCLUSIONS: This study emphasizes the need to be aware of the possibility of cases of PKDL in endemic regions of leprosy, as the conditions may be difficult to distinguish clinically and histopathologically.

Adolescent↗