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Biomedical subjects

V J Yates

Publications and source records attributed to V J Yates.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

The presence of avian adenoviruses and adeno-associated viruses in healthy chickens.

Avian adenoviruses were isolated from 56 of 106 fecal and rectal tissue samples taken from apparently healthy young chickens on 4 farms. Only one isolation was made from the 37 samples from 3- and 4-week-old chicks, while the isolation frequency was 64-100% in groups 5 weeks old and older. The 56 adenovirus isolates were classified into 6 serotypes. Vurses of 3 or 4 types were found on each farm. Avian adeno-associated virus CF antigens were found, using chicken embryos coinfected with an adenovirus helper, in 2 of the 38 adenovirus isolated studied.

Adenoviridae↗
PubMedDate not supplied

Hemagglutinating properties of CELO, an oncogenic avian adenovirus.

Chicken-embryo-lethal-orphan (CELO) virus, Phelps strain, agglutinated erythrocytes at 37 C. The hemagglutinating activity, which is a function of complete and incomplete virus particles, was sensitive to heat but not to pH. The soluble components of the virus were similar in sedimentation characteristics to those obtained from human adenovirus type 1. The effects of chemical and physical agents on CELO hemagglutinin, CELO infectivity, and red-cell receptors suggested that the last were protein in nature and that cell-virus attachment was mediated by amino groups on the virion. The attachment of virus to red blood cells via the penton projection was demonstrated by electron microscopy.

Adenoviridae↗
PubMedDate not supplied

Serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.

The avian adenoviruses (AV) are common infectious agents of poultry and other avian species throughout the world (1,4,8). Limited observations suggest that the adeno-associated virus (A-AV) coinfects many of the chickens that carry AV (8). The presence and persistence of these infections in a flock is often determined by serological methods. In the current study, the immune response of chickens to type 1 AV alone and to a dual exposure, AV plus A-AV, was followed over a 12-week period with a variety of serological tests. The study also determined the duration of the viral infections.

Adenoviridae↗
PubMedDate not supplied

An in vivo and in vitro study of infectious laryngotracheitis virus in chicken leukocytes.

Infectious laryngotracheitis (ILT) virus, an avian herpesvirus, caused an infection in chickens that was followed by leukopenia due to a decrease in the number of circulating lymphocytes. Viral synthesis in leukocytes in cell cultures was evident by specific and progressive viral antigens in the nuclei of infected leukocytes as shown by fluorescent-antibody technique and by the formation of multinucleated giant cells, typical of the herpesviruses. In addition, viral multiplication was observed in the leukocyte cell cultures as shown by viral assay. It is suggested that the leukocytes in chicken are participating in the production of ILT virus by serving as host cells for viral multiplication. This is followed by a cytopathogenic effect, with the production of multinucleated giant cells. That may result in the destruction of cells, causing a leukopenia.

Animals↗
PubMedDate not supplied

Effect of avian adeno-associated virus on pathogenicity of Tipton virus in chicks.

The avian adeno-associated virus (A-AV) reduced the pathogenicity of an adenovirus infection in vivo. Groups of chicks were infected with Tipton virus alone or in combination with high or low doses of A-AV. In both trials, the associated virus delayed and reduced chick mortality. This effect was dose-dependent and significant at the higher dose level.

Adenoviridae Infections↗
PubMedDate not supplied

Use of egg yolk to determine antibody levels in chickens inoculated with a hemagglutinating duck adenovirus (adenovirus 127-like).

Chickens were experimentally infected with a duck adenovirus that has been shown to be serologically indistinguishable from Adenovirus 127. Sera and eggs were collected at intervals after exposure for antibody determination by the hemagglutination-inhibition (HI) test, the enzyme-linked immunosorbent assay (ELISA), and the immunodiffusion (ID) test. Egg yolks were processed for use in the serological tests by (a) dilution in phosphate-buffered saline (PBS), (b) extraction of the water-soluble fraction with chloroform, or (c) freezing and thawing PBS-diluted yolks and testing the supernatant fluid. HI antibody titers from serum and extracted yolk were similar except during the initial 2 weeks, when yolk antibody levels were low or absent. Chloroform-extracted yolks were suitable material for the HI, ELISA, and ID tests. Heat inactivation of the chloroform-extracted yolk had no effect on titers.

Adenoviridae↗