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Biomedical subjects

V I Vasil'eva

Publications and source records attributed to V I Vasil'eva.

At least 19 recordsLinked to original sources
PubMed2006

[Molecular typing of the strains of Bordetella pertussis circulating in St. Petersburg during an outbreak of the disease].

Growth of the whooping-cough morbidity during the last years in Russia and other countries with 40-year-long history of immunization gave rise to significant interest of researchers to variability of the Bordetella pertussis population. Comparative assay of the genomes of the B. pertussis strains circulating in St. Petersburg in 1998-2000 and strains used to produce domestic vaccines AKDS was performed using the pulse-field electrophoresis and sequencing. It was found that most strains of B. pertussis circulating during this period were distinguished from the vaccine strains by the DNA-profile and structure of genes involved in encoding of biosynthesis of the S1 subunit of the whooping-cough toxin (ptxS1) and pertactin (prn). It was shown that 62% of wild-type strains had electrophoretic profiles IV alpha and IV beta, whereas vaccine strains had electrophoretic profiles II and III. Circulation of strains with profiles IV alpha and IV beta was found to correlate with the whooping-cough morbidity rate in vaccinated children. Our results and data of other researchers were compared and analyzed.

Adolescent↗
PubMed1992

[The factors that affect the results of the serological diagnosis of HIV infection and the screening for anti-HIV].

The laboratory diagnosis of HIV infection and screening for anti-HIV have determined the factors that influence the results of studies performed by ELISA and Western immunoblot. The factors include sensitivity and specificity of the test systems used, storage conditions of and transportation conditions for serum, errors of procedures for reactions, etc. Correlation is between the inactivation of the sera studied and the initial antibody titers during their storage at elevated temperatures. To obtain more valid results of HIV infection serodiagnosis it is necessary to use some test systems differing in the principles of setting up a reaction.

AIDS Serodiagnosis↗
PubMed1991

[The evaluation of the late effect of the multiple administration of immunoglobulin on hepatitis A morbidity in organized collectives].

The analysis of influence in multiple injections of immunoglobulin (IG) on a level of morbidity and the efficiency of IG prophylaxis against hepatitis A in organized collectives made it possible to reveal the following common features: there is a direct dependency between morbidity level and average geometrical anti-IG titers, as well as the number of injections; there is inverse dependency between IG protection activity and the same factors; there is a lack of prophylaxis effect in anti-IG titers 1:32 or more. The authors frame a hypothesis that the reason of epidemic upgrowth of long standing hepatitis A morbidity dynamics in military collectives was conditioned by the wide application of IG in the 70s for children and teenagers.

Age Factors↗
PubMed1991

[The use of computers and mathematical methods for optimizing epidemic control measures with respect to measles infection].

The potentialities of computers for the study of the effectiveness of immunization have been demonstrated and the mathematical model for the prediction of the proportion of children, seronegative to measles, derived on the basis of the data on the average measles morbidity in different groups for a given period. A multifactor analysis of a large scope of data obtained in seroepidemiological survey and a retrospective analysis of measles morbidity on the basis of data collected in two districts of Moscow have been carried out with the use of computers and mathematical methods.

Age Factors↗
PubMed1991

[A comparative evaluation of the immunological efficacy of ADPT vaccine and ADT-M anatoxin in the immunization of children against diphtheria and tetanus].

Comparative study of the immunological effectiveness of adsorbed diphtheria-pertussis-tetanus (DPT) vaccine and adsorbed diphtheria-tetanus toxoid with reduced antigen content (adsorbed DT toxoid R) in the immunization of children, carried out in accordance with the vaccination schedule, was made. Immune response to the injection of adsorbed DPT vaccine was higher than after immunization with adsorbed DT toxoid R, as evidenced by antibody titers. It was probably due to differences in the number of injections constituting the course of immunization: it consisted of 3 injections and 1 booster injection for adsorbed DPT vaccine and 2 injections and 1 booster injection for adsorbed DT toxoid R. Immunization with adsorbed DPT vaccine produced immunity which was retained for a longer period. These results are indicative of the expediency of the primary immunization of children with adsorbed DT toxoid R introduced in three injections in order to ensure more stable and prolonged postvaccinal (mainly antidiphtheria) immunity.

Antibodies, Bacterial↗
PubMed1991

[The validation of the expediency of a review of the times for revaccinating children against measles].

Basing on the results of seroepidemiological study, carried out in two districts of Moscow by different methods, cluster selection method including, the authors have developed the following recommendations aimed at improving the strategy of revaccination against measles: (1) selective revaccination of only seronegative children or those with poor antimeasles immunity should be carried out, thus making it possible to reduce the number of susceptible children and diminish the risk of postimmunization reactions and complications; (2) when determining the groups of children to be revaccinated and the age suitable for revaccination, one should bear in mind the specific local features of the epidemic process in measles and the morbidity values, as well as the data on antimeasles immunity in children of different age groups; (3) serologic monitoring of the quality and immunologic effectiveness of different batches of live measles vaccine permits timely removal of nonstandard batches from practical use, thus improving the efficacy of vaccinal prophylaxis of measles.

Adolescent↗
PubMed1990

[Serological and epidemiological characteristics of acute pneumonias caused by Mycoplasma pneumoniae in Belgorod].

The etiology of the outbreaks of acute pneumonia in Belgorod was established and their epidemiological features were studied. The mycoplasmal etiology of all cases of acute pneumonia in children and adults, appearing alongside acute respiratory infections resulting from the preceding outbreaks caused by influenza viruses A/Prague/, B/Leningrad/369/75, and all types of parainfluenza viruses, was shown. The droplet mechanism of the transfer of infection was established, which was confirmed by the severity of the outbreak and a high rate of infection of the medical staff at hospitals for children and adults, where patients with mycoplasma-induced pneumonia were treated.

Acute Disease↗
PubMed1990

[The immune structure of occupational groups of the population in relation to Legionella pneumophila serogroup 1].

The immune structure of different professional groups with respect to L. pneumophila has been studied. A wide spread of Legionella infection among subway construction workers, subway personnel and railroad conductors has been established, which makes it possible to regard these professions as high risk groups susceptible to Legionella infection. The risk factors for this group are as follows: work in the atmosphere of high dust content with air supply by means of air conditioners, contact with soil in the process of earthwork. The bacteriological study of water samples obtained in dining cars has resulted in the isolation of two strains of L. pneumophila, serogroup I, which confirms the possibility of cases of Legionella infection among railroad personnel.

Adult↗
PubMed1990

[Clinical picture and treatment of Legionella pneumonia in children].

Data on the clinical and laboratory study involving 150 children with the verified diagnosis of pneumonia observed as inpatients within a period from 1984 to 1988 are presented. To elucidate the pneumonia etiology, blood sera to the causative agents of influenza and parainfluenza, adenovirus, RS virus, Mycoplasma pneumoniae and Legionella pneumophila were assayed. An increase in the number of neutralizing antibodies to L. pneumophila was observed in 17.3 per cent of the patients. The majority of them simultaneously showed positive serological shifts with respect to the respiratory viruses. A specific clinical picture of pneumonia in the patients with the confirmed legionella infection was stated. The description of the treatment results is retrospective.

Adolescent↗
PubMed1990

[Legionella infections in children].

The authors provide the results of observing 150 patients with acute pneumonia verified on x-ray. Comprehensive serological studies enabled the legionellosis etiology of pneumonia to be established in 17.3% of cases in sporadic morbidity of children. The disease was marked by the peculiarity of the clinical manifestations, demanding nonstandard treatment approaches.

Antibodies, Bacterial↗
PubMed1988

[Comparative study of a diagnostic agent for detecting the antigen of the hepatitis A virus: dried erythrocyte immunoglobulin].

The results of the trials of dried erythrocyte immunoglobulin immunodiagnosticum for the detection of hepatitis A virus antigen, developed by a group of scientists at the Tashkent Research Institute for Vaccines and Sera, are presented. The immunodiagnosticum is specific to hepatitis A virus antigen and suitable for the detection of this antigen in fecal and urine samples from patients with viral hepatitis A in the acute stage of the disease.

Acute Disease↗
PubMed1988

[Etiologic structure of viral hepatitis in children based on data from an comprehensive serologic study of hospitalized patients].

During a year an overall serological examination of 1,200 children hospitalized with the diagnosis of viral hepatitis was carried out with a view to establish the presence of hepatitis A and hepatitis B markers (anti-HAV IgM, HBsAg, anti-HBs, anti-HBc and anti-HBe IgM) by means of the enzyme immunoassay and radioimmunoassay. The average annual proportion of cases of hepatitis A (61.4%), hepatitis B (20.4%), hepatitis non A, non B (11.2%) and mixed infection (7.0%) among children, as well as seasonal and age-group fluctuations of this proportion, were established. The etiological structure of viral hepatitides, based on commonly accepted clinico-epidemiological diagnostic criteria, was shown to differ from their true etiological structure as determined in accordance with specific serological markers detected in this investigation.

Adolescent↗