Search PubMed⌕ Search

Biomedical subjects

V Herbert

Publications and source records attributed to V Herbert.

At least 109 records · Page 6Linked to original sources
PubMed1981

Effect of low dose of cimetidine on gastric potentials difference and acetylsalicylic acid-induced change.

Cimetidine in a low dose of 10 mg given intravenously increases significantly the gastric potential difference (GPD) without any inhibitory effect on gastric acid secretion. The increase of GPD after 200 mg cimetidine is higher than after 10 mg. While the high dose of cimetidine prevents the acetylsalicylic acid (ASA)-induced GPD drop, cimetidine in a dose of 10 mg is not able to protect this change. Nevertheless the low dose of cimetidine may have a clinical significance and should be evaluated by clinical studies.

Adult↗
PubMed1981

Detection of a milk factor that facilitates folate uptake by intestinal cells.

Folate binding proteins in milk were tested for their effect on folate absorption. The uptake of bound folate by isolated mucosal cells from the rat small intestine was twice that of free folate and differed from it in being more effective with progression down the small intestine, in not being affected by glucose or Dilantin, in having a higher pH optimum, and in being affected by calcium concentration. This milk factor may enhance folate absorption in infants, whose risk of folate deficiency is high.

Animals↗
PubMed1981

Vitamin B12.

Explore the source record for details and available documents.

Ascorbic Acid↗
PubMed1980

In vitro DNA synthesis by bone marrow cells and PHA-stimulated lymphocytes. Suppression by nonradioactive thymidine of the incorporation of 3H-deoxyuridine into DNA: enhancement of incorporation when inadequate vitamin B12 or folate is corrected.

Previous studies demonstrated that excess deoxyuridine (dU) added to short-term bone marrow and PHA-stimulated lymphocyte cultures, blocks the incorporation of radioactive thymidine into DNA via the salvage pathway. In the current study, we investigated the effects of added thymidine (TdR) in varying concentrations (10(-6) to 1 mumol) on the incorporation of 3H-dU into thymine-DNA, i.e. we executed 'thymidine suppression tests.' Increasing concentrations of exogenous TdR caused progressive inhibition of 3H-dU incorporation into DNA, and decreasing 3H-dU incorporation was parallelled by increasing incorporation of added 14C-TdR. These findings demonstrate reciprocity of the salvage and the de novo pathways of thymine-DNA synthesis, presumably mediated by thymidine-triphosphate (dTTP), the common end product of both pathways, via feedback inhibition. In patients with folate and/or vitamin B12 deficiency, the addition of appropriate vitamins to marrow and lymphocyte cultures enhanced the incorporation of 3H-dU into DNA. As predicted, this was not observed in normal subjects. The enhancing effect of these vitamins on in vitro incorporation of 3H-dU into DNA by deficient cell systems was similar to their correcting effect on abnormal dU suppression. These findings support the theoretical concept that the dU suppression test defines biochemical megaloblastosis due to deficiency of folate and vitamin B12.

Bone Marrow↗