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Biomedical subjects

V Gulani

Publications and source records attributed to V Gulani.

5 recordsLinked to original sources

A simple geometrical description of the TrueFISP ideal transient and steady-state signal.

An intuitive approach is presented for assessment of the TrueFISP signal behavior in the transient phase and the steady state, based on geometrical considerations in combination with the Bloch equations. Short formulations are derived for the zenith and phase angle determining the direction of the magnetization vector for which a smooth monoexponential decay is obtained even at considerable off-resonance frequencies, thus compactly defining the target of various preparation schemes proposed in literature. A pictorial explanation is provided to illustrate how the interplay between RF excitation and relaxation governs the TrueFISP transient phase and steady state. Closed form expressions are developed that describe the signal evolution, accounting for the influence of T(1), T(2), flip angle, and resonance frequency offset in agreement with recently published studies. These results are obtained directly from basic assumptions, without the need for abstract mathematical treatment or further approximations. The validity of the conceptual framework and the analytical description is verified by simulations based on the Bloch equations as well as with MR phantom experiments. The theory may be used for contrast calculations and has the potential to facilitate improved parameter quantification with magnetization prepared TrueFISP experiments accounting for off-resonance effects.

Image Processing, Computer-Assisted↗

Apparent diffusion tensor measurements in myelin-deficient rat spinal cords.

The apparent diffusion tensor (ADT) was measured in excised and fixed spinal cords from myelin-deficient (md) rats and age-matched controls. These data were used to obtain the principal diffusivities of the ADT, and also the scalar invariant parameters _D (averaged principal diffusivity) and A(sigma) (anisotropy index) for four white matter and two gray matter regions. The results for white matter regions showed that the principal diffusivities were significantly higher for md animals, and while the _D was increased in tissue from md animals, the A(sigma) was found to be decreased. Grey matter _D was measured to be between those of white matter from control and md animals, and the A(sigma) was much smaller than that of white matter from both sets of animals, indicating that diffusion in md white matter is more anisotropic than in gray matter. The results show that while myelination is not a prerequisite for diffusion anisotropy, it does influence the magnitude of the observed anisotropy. Magn Reson Med 45:191-195, 2001.

Animals↗

Magnetic resonance microscopy of morphological alterations in mouse trabecular bone structure under conditions of simulated microgravity.

This work describes the use of magnetic resonance (MR) microscopy to examine changes in tibial trabecular bone structure in mice following 28 days of hindlimb suspension, a model simulating the effects of microgravity in rodents. In this first MR study involving mice, analysis of 3D images showed that apparent bone volume fraction, trabecular number, and trabecular thickness were decreased, and apparent trabecular spacing increased, significantly (P < 0.05) in hindlimb-suspended mice compared to controls. These changes agreed well with light microscopy measurements from an independent study and also with actual spaceflight experiments with rats.

Animals↗

Apparent water diffusion measurements in electrically stimulated neural tissue.

The effect of stimulation on diffusion characteristics of electrically stimulated neural tissue was examined. Bullfrog nerves and spinal cords were excised and stimulated electrically during magnetic resonance imaging, and the apparent diffusion coefficients (ADCs) of water parallel and perpendicular to the long axes of the specimens were mapped with and without stimulation of the tissue. Electrophysiological recordings were used to determine the viability of the tissue after each experiment. No stimulus-related ADC changes were observed in either the peripheral nervous system or the central nervous system tissue. These experiments may help to define further the nature of previously reported ADC changes in stimulated neural tissue in situ.

Animals↗

A multiple echo pulse sequence for diffusion tensor imaging and its application in excised rat spinal cords.

A new imaging sequence for rapid determination of the apparent self-diffusion tensor of water was developed and tested on fixed excised rat spinal cords. To reduce the time required to determine the tensor, the sequence utilized a new single-shot approach with multiple spin echoes. An assumption of cylindrical symmetry in the sample was made, thus requiring the measurement of only four of the six unique elements of the tensor. This assumption was found experimentally to be valid, and the results obtained using the new sequence were found to be quantitatively the same as results obtained using a standard spin-echo sequence.

Animals↗