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Biomedical subjects

V G Vladimirov

Publications and source records attributed to V G Vladimirov.

At least 19 recordsLinked to original sources
PubMed2001

Postradiation DNA polymorphism.

The presence of DNA molecules with tumor-specific genetic alterations has been demonstrated in the plasma of cancer patients. Such plasma DNA fragments may be immunogenic and provide immunologic support to the malignant cells. Further, the tumor-specific plasma DNA may participate in the adaptive processes of the tumor cells in the cancer host. The mechanisms of the latter are unclear. However, from the standpoint of the resistance problem, understanding of DNA transformation after exposure of extreme factors is of great interest.

Animals↗
PubMed1993

[The reciprocal aggravation syndrome as the most important characteristics of combined radiation lesions].

This article studies the peculiarities of pathogenesis and the syndrome of reciprocal aggravation in combined radiation injuries. The authors make the analysis of various methods for quantitative estimation of this syndrome. The article substantiates the necessity to carry out the quantitative evaluation of gravity factor in combined radiation injuries in order to render an adequate health care to wounded during various stages of medical evacuation.

Acute Disease↗
PubMed1992

Extracellular DNA level in the blood of irradiated rats.

There is high-molecular DNA in the blood of unirradiated rats which moves as a single fraction during electrophoresis in 0.5% agarose. At short times (2-5 h) after gamma-irradiation at doses from 1 to 100 Gy a low-molecular species of DNA appears (about 180 nucleotide pairs), the amount of which is directly proportional to exposure dose at 5 h after exposure. It has been established by Southern hybridization that the low-molecular DNA has few nucleotide sequences common with those of the high-molecular DNA, but it shows homologously to genomic sequences.

Animals↗
PubMed1992

[Contents of extracellular DNA in blood of irradiated rats].

Intact rat plasma contains high-molecular DNA which moves as a single fraction in 0.5% agarose electrophoresis. As early as 2-5 hours after gamma-irradiation in a dose of 1-100 Gy there appears low-molecular DNA (about 180 nucleotide pairs), the amount of which directly correlates with the irradiation dose 5 hours after the exposure. Blot-hybridization showed that low-molecular DNA has no common nucleotide sequences with high-molecular DNA, though has sites similar to genome repeat sequences.

Animals↗
PubMed1991

[Separation of bone marrow cells in a Percoll discontinuous density gradient].

Human bone marrow cells were separated by centrifugation in percoll discontinuous density gradient with 1.020-1.080 g/ml densities. CFU-GM test and EPICS analysis were used to characterize cell compositions of the resultant fractions. The employed mode of separation permits obtaining cell fractions rich for monocytes, macrophages, T and B lymphocytes.

Bone Marrow Cells↗
PubMed1989

[The information value of clinico-hematologic criteria for the early diagnosis of acute radiation sickness in pig-tailed macaques].

Ten pig-tailed monkeys (Macaca nemestrina) were subjected to 60Co radiation at a dose of 6.0-6.5 Gy and a dose rate of 1.2 Gy/min. Acute radiation sickness has developed in the monkeys causing their death on the 16-20 day. In spite of this, the initial reaction was weakly expressed and according to its manifestation it was impossible to evaluate severity and possible outcome of the lesion. At an early stage of the disease (6-24 hours) insufficient was uranin fluorescence in blood plasma, but more informative were the changes in adhesive properties of leukocytes the dynamics of lymphocytes (lymphopenia), reticulocytes (reticulocytopenia) and shifts in reticulograms (increased per cent of juvenile forms).

Acute Disease↗
PubMed1988

[Surgical tactics in anaerobic non-clostridial peritonitis].

Under observation there were 128 patients with neclostridial peritonitis (NP) which made up 19.3% of the total number of patients with peritonitis. In spreaded NP when the source of NP was successfully eliminated and fibrinous layers on the peritoneum the laparotomy wound was tightly sutured up to the aponeurosis (included), primary delayed sutures were put on the skin. When it was impossible to completely remove all the fibrin from the peritoneum, the wound was sutured through all the layers, the sutures were tied in bows followed by numerous revisions of the abdominal cavity. Laparotomy was performed in postoperative NP with phlegmons of the abdominal cavity. The use of the tactics described allowed lethality to be reduced from 39.4% to 13.7%.

Anti-Infective Agents, Local↗