[Interrelations of blood clinical indices in fractures of bones of the supporting motor apparatus].
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Biomedical subjects
Publications and source records attributed to V G Shamratova.
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The factor analysis was used to examine a relationship of a number of clinical and blood biochemical parameters to the level of triiodothyronine (T3), tyroxine (T4), and thyroid-stimulating hormone (TSH) in third-fourth degree diffuse toxic goiter (DTG) in females aged 17-45 and 46-58 years. It was established that the changes in T4 and TSH values were coordinated and T3 values varied independently. In DTG, the concentration of T4 correlated with that of K' and the levels of TSH and T4 correlated with those of cholesterol only in the age group less than 45 years. The specific features of the leukogram were associated with the level of T4 in females aged under 45 years and with that of TSH in females over 45 years of age. In DTG, there was a drop in blood TSH concentrations only in the group of 46-59 years.
A significant curvilinear relationship was found between erythrocyte count in rat blood and electrokinetic characteristics of these cells. Electrophoretic mobility of erythrocytes remained unchanged, slightly increased, or decreased with increasing cell count in the vascular bed depending on animal state. Excessive increase in the number of erythrocytes was accompanied by accumulation of cells with low electrophoretic mobility in the electric field.
In intact rats electrokinetic characteristics of erythrocytes weakly depended on the intensity of erythropoiesis. This dependence became significant when the degree of erythropoiesis approached the upper limit of variations. Electrophoretic mobility of erythrocytes progressively decreased with increasing blood reticulocyte count. Subpopulations of erythrocytes with low mobility in electric field accumulated in the blood. Our results indicate that erythrocytes whose electrokinetic potential is similar to the mean mobility of circulating cells migrate from the bone marrow into the circulation under normal conditions.
The interrelationship between electrophoretic mobility and the volume of rat blood erythrocyte is described by a non-linear curve, where the deviation of cell volume from the physiological normal is accompanied by a decrease in their mean electrophoretic mobility. In some ranges, the deviations of erythrocyte volume do not affect their electrokinetic properties. It can be hypothesized that the simultaneous shifts of these parameters are caused only by some hematological abnormalities that are manifested in pronounced changes in cell homeostasis.
It was established, while making blood tests for healthy males and for patients with glomerular nephritis at exacerbation, that there is a statistically reliable interrelation between the erythrocyte electrophoretic mobility and their sedimentation velocity. Its nature is subject to modifications as the functional body state and the investigated range of concurrently altering parameters are changing. Such hierarchy, in neuropathy, can be also the case at the level of mean parameters; and in health, it can be observed only as a conjugated reconstruction of the erythrocyte population structure.
The potentialities of optimizing the clinical use of electric surface properties of erythrocyte blood samples were investigated. The ordinarily used parameter of the eletrokinetic erythrocyte potential based on the mean value of electrophoretic mobility was shown to be inaccurate and low informative. Asymmetry factor and erythrogram excess (distribution parameters) are recommended for use to enhance the adequate evaluation of changes in the above parameter and to make it diagnostically more effective. Principles of interpretation of changes' results are described.
Using the factor analysis, we studied the influence of psychoemotional strain, experienced by students under taking examinations, on the electrophoretic mobility of their erythrocytes. Under stress condition, redistribution of shares of cells with different mobility occurs, directed to the maintenance of the optimal value of the index average level in the total pool of erythrocytes of an individual. Under stress, five factors, taken in different combinations, participate in the control of erythrokinetic properties: those of restriction of cell accumulation with abnormal mobility, and of the population quantity heterogeneity control, in addition to factors of total functional condition, emotional tension, and individual psychological steadiness of students before examination. The expression and character of stress influence on the state of erythrocyte population depend on the intensity of the functional load of the organism.
The potentialities of increasing the resolving capacity of standard hematological parameters used for the diagnosis of chronic nonspecific ulcerative colitis were investigated. The mean volume of erythrocytes decreases and the shape of population erythrogram is modified in this disease. White blood is characterized by qualitative changes (mean cell volume and parameters of its distribution for neutrophils lymphocytes, and monocytes). Consideration for the corpuscular volume of these cells helps differentiate between slight, medium, and severe chronic nonspecific ulcerative colitis.
As assessed by changes in the erythrograms asymmetry coefficient, selectivity of the strophantin effect on cell populations with different mobility and age specifics of reciprocal responses, was revealed in adult subjects and in children. The data obtained suggest existence of difference in quality of functional condition of the erythrocytes with normal as well as reduced mobility in children and in adults.
An interaction was shown to exist among the quantities of neutrophiles, lympho- and monocytes in circulation and characteristics of their corpuscular volume. The correlation embraces all the leukocyte classes, being apparent in indices of separate classes as well as on the intertype level. Such a co-ordination is involved in maintenance of the normal microcirculation level and manifested in a series of intra- and intertype correlations of relative content and parameters of the corpuscular volume distribution of the cells.
Total concentration of erythrocytes and leukocytes, a relative contents of neutrophils, lymphocytes, monocytes, average volume of these types of cells, were determined in humans. Changes in the cell indices seem to be correlated and sex-dependent. Thus, erythrocytes and leukocytes are correlated in their contents and volume characteristics. A control mechanism seems to exist in humans, involving microcirculation in capillaries.
The mean corpuscular volume and concentration of blood erythrocytes in intact male rats are inversely related in the entire fluctuations range. In healthy men and women the correlation between these parameters is described by a parabola with alternating zones of positive and negative relationships. These covariations are unstable; in disease they change and sometimes are transformed into monotonous reciprocal correlations.
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On model of scald trauma the correlation of electrophoretic mobility of rat blood erythrocytes with intensity of lipid oxidation of erythrocyte membranes and with activity of catalase were studied. It is shown that in dynamics of scald disease development the erythrocyte mobility decreases, the velocity of oxidizing ability of their lipids increases and the protective action of blood antioxidation system is blocked. It is found that there is a close dependence between the level of oxidative processes and electrogenic properties of erythrocytes at the height of manifestation of scald toxaemia. A conclusion was made that the oppressing influence of increasing lipid oxidizing ability a on electrokinetic potential of erythrocytes is of a threshold nature and is realized through change in the membranes' state of these cells.
The electrophoretic mobility of children blood erythrocytes in norm and with iron-deficiency anemia was studied. The age peculiarities and sex specificity of this index was revealed, demonstrated chiefly by dynamics of shape of its distribution curve reflecting a ratio of cell subpopulations with different electrokinetic potential. The state of anemia is accompanied with modification of histogram profiles of both individual and group cell mobility, with patterns of changes also depending on the child sex. The obtained data suggest that at children iron-deficiency anemia the structure or functional status of erythrocyte membrane are changed, but the pathological process does not eventually involve all the populations of circulating erythrocytes.
A correlation between erythrocyte electrophoretic mobility (EEM) and a complex of clinico-biochemical indices in the blood of patients with chronic pyelo- and glomerulonephritis was studied. It is established that the EEM homeostasis as a whole, comes to the redistribution of cell subpopulation parts with high and low mobility. For keeping stability of EEM, of great significance in the control patients is the total protein content in the plasma, whereas in patients with kidney pathology of primary importance is the level of neutrophils.