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Biomedical subjects

V G Gololobov

Publications and source records attributed to V G Gololobov.

At least 19 recordsLinked to original sources
PubMed2005

[Participation of transfused bone marrow cells in reparative osteohistogenesis].

The participation of skeletal tissue cell precursors in the repairing regeneration of bone tissue was studied. Bone marrow was taken from donor animals--mice of C57Bl/6-TgN(ACTbGFP) 1 Osb line (The Jackson Laboratory Bar Harbor ME USA line). Nucleated cell fraction was isolated by centrifugation on a density percoll gradient. Recipient mice C57Bl/6 line were irradiated by 7.0-7.5 Gr dose. Intravenous infusion of donor cells and osteoclasts of tibia was done after irradiation of recipient mice. Histological preparations of bone regenerate tissues were studied on 15, 30, and 60 days by confocal microscopy. Donor cells were found as skeletal tissue precursors into periost, endost, bone marrow, and as differentiated cells of newborn tissue of regenerate--osteoblasts, osteocytes, chondrocytes. The data obtained indicate that part of donor bone marrow cells are able to progressive differentiation under recipient bone fractures.

Animals↗
PubMed2004

[Development and morphofunctional characterization of the osteoblastic phenotype in cell culture in vitro].

The objective of this research was to study osteogenic properties of cultured rabbit bone marrow stromal cells, newborn rat cranium bone cells and rat osteocarcoma ROS 17-2/8 cells. For this purpose cytochemical reaction for alkaline phosphatase was performed by the Lowry method, mineral deposition was assessed by staining of the cultures after von Kossa. Cranium bone cells were shown to synthesize alkaline phosphatase (34 +/- 7 nmol/min/10(6) cells), the matrix mineralization being found. Bone marrow stromal cells displayed a lower activity alkaline phosphatase level than did cranium bone cells (4 +/- 0.6 nmol/min/10(6) cells). However, cell cultivation in the presence of dexamethasone in the medium (10(-8) M) induced a higher activity of alkaline phosphatase (9 +/- 1 nmol/min/10(6) cells), mineralization of the extracellular matrix being the case. The highest level of alkaline phosphatase activity was found for ROS 17-2/8 cells (60 +/- 12 nmol/min/10(6) cells) but no matrix mineralization was determined. According to these data, matrix calcification and formation of bone-like nodules are the most important properties of osteoblastic differentiation in vitro.

Alkaline Phosphatase↗
PubMed2004

[Lamellar bone culture in vitro].

The objective of this study was to analyze morpho-functional characteristics of the cells in definitive osseous tissue cultured in vitro. Cortical plates, isolated from the fragments of iliac bones of adult rabbits, were cultured in vitro in a nutrient medium. The culture obtained was fixed after 3, 10 and 20 days and processed for scanning electron microscopy. To identify the osteogenic cells, alkaline phosphatase activity was demonstrated. It was established that the processes of cell proliferation and migration from the cultured bone pieces were most active at 10 days after the explanation. The growing cells formed a growth zone around the bone fragment that consisted of three layers, differing in the degree of cellular proliferation and differentiation. After 20 days in culture the cells expressing alkaline phosphatase were shown to form groups. The cells of osteon canals in culture were found to possess potential for proliferation and osteogenic differentiation.

Alkaline Phosphatase↗
PubMed2003

[Stromal stem cells and osteoblastic cellular differon].

The article presents an analysis of the data on the origin, differentiation, morpho-functional characteristics and reactivity of the bone cells taking into account theoretical concepts of histogenetic series and cell differons. The information on the sources and proliferation of cells of the osteogenic line was used as the basis for development of a variant of a scheme describing the differentiation of these cells from a stromal stem cell (SSC) to a bone-lining cell and osteocyte. Considering possible differentiation of SSC into the cells of fibroblastic, chondroblastic, adipocytic and leiomyocytic cell lines, the unified model of mechonocytogenesis is proposed.

Cell Differentiation↗
PubMed1997

[Regeneration osteohistogenesis in the healing of gunshot fractures (an optical polarization analysis of the collagen fibers in the intercellular substance)].

Bone tissue intercellular substance was studied in healing of a tibial gunshot fracture in dog. The method of scanning polarization microscopy was used with cytospectrophotometer signal registration and the following three-dimensional computer reconstruction of indexes of the process which was monitored from days 15 to 120 of the experiment. By the end of the experiment optic anisotropy indexes of collagen fibres from proximal and distal bone fragments were 96 and 80 respectively as related to intact bone. Character of scanogrammes and increasing values of anisotropy indexes of fibrous component of the regenerate intercellular substance indicate changing and normalizing fibrillar architectonics of newly formed bone tissue on the site of preceding fracture and serve as one of criteria for regenerative osteogenesis phases.

Animals↗
PubMed1996

[Bone tissue regeneration after a gunshot fracture].

A general histological analysis of regularities of the osseous tissue reparative regeneration was performed in dops with gunshot fracture of long tubular bones. Methods of light and electron microscopy, cytophotometry of summary proteins optic density, historadioautography with 3H-thymidine were used. On the site of the osseous defect forms a complicated in tissue composition regenerate in the course of proliferative stage of regeneration and in interaction of diverse cellular differons. Viable osteogenic elements of bony fragments are the accessory source of the osseous regenerate forming. A mechanism of regenerative endosteal osteogenesis is revealed. Realization of the osseous tissue histoblastic properties, intertissue correlations and remodeling of the regenerate lead to healing of the gunshot fracture.

Animals↗
PubMed1990

[Cell-differon organization of the tissues and the problem of wound healing].

Problems on correlation of processes of normal histogenesis and those of physiological and reparative regeneration are discussed. A special attention is paid to the cellular-differon organization of tissue and its significance for understanding cellular mechanisms and ultrastructural bases in tissue regeneration after lesions and use of biostimulators of various origin. Certain peculiarities have been revealed in changes of intra- and interdifferon interrelations of cells in various tissues at regenerative histogenesis. The biostimulators applied differently change duration and manifestation of its stages, that is they influence the internal structure of the process and the cellular-tissue composition of the regenerate.

Animals↗
PubMed1988

[Reactive changes in the tissues of the skin and its appendages in different methods of transplanting full-thickness flaps].

Free epidermoplasty and skin transplantation is performed on microarterial and microvenous anastomoses. Tissue reactions are studied in 3-21 days. Transplantation of a complex graft with application of microvascular anastomoses possesses definite advantages in comparison with a traditional method of free plasty. They are demonstrated as a complete survival of the transplant tissues, its revascularization, predominance of regenerative processes over the destructive ones in the epidermis, derm and appendages of the skin, preserved viability of tissue components in hair and sebaceous glands. All this is of certain importance for normal functioning of the skin as an organ, as well as an essential cosmetic importance.

Animals↗