Data presented here indicate that iron exposure exacerbates the changes in the glutathione status of the liver cell and the lipid peroxidative response of the tissue induced by acute ethanol intoxication. In these conditions, additivity in lipid peroxidation was found to occur either when the process was estimated by hepatic malondialdehyde formation, as well as by measurements of the biliary malondialdehyde release in the anesthetized rat and by the antioxidant-sensitive respiration in the perfused rat liver, procedures which are non-invasive for the tissue.

Animals↗

PubMedDate not supplied

Genetic variation between susceptible and non-susceptible snails to Schistosoma infection using random amplified polymorphic DNA analysis (RAPDs).

Susceptibility of snails to infection by certain trematodes and their suitability as hosts for continued development has been a bewildering problem in host-parasite relationships. The present work emphasizes our interest in snail genetics to determine what genes or gene products are specifically responsible for susceptibility of snails to infection. High molecular weight DNA was extracted from both susceptible and non-susceptible snails within the same species Biomphalaria tenagophila. RAPD was undertaken to distinguish between the two types of snails. Random primers (10 mers) were used to amplify the extracted DNA by the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The results suggest that RAPD represents an efficient means of genome comparison, since many molecular markers were detected as genetic variations between susceptible and non-susceptible snails.

Animals↗