Against the background of the induced iron deficit ethanol (6.4 g/kg) causes aggravation of the embryolethal effect and anomalies in 15% of embryos in 14-day pregnant rats. Changes in the genome of rat males and females after the injection of the plasmid with a foreign gene at the stage of two pronuclei and the subsequent crossing with intact animals account for the increase in sensitivity of embryos to subteratogenic doses of sodium salicilate. The maternal organism disturbances have a more pronounced effect than the paternal ones.

2,2'-Dipyridyl↗

PubMedDate not supplied

[The prevention of congenital developmental anomalies in rats].

Preventive administration of Eleutherococcus XXX extract during prenatal and pre-embryonic periods of development prevents embryotoxic effect of subsequent treatment of pregnant rats with ethanol and sodium salicylate. Eleutherococcus abolishes embryotoxic and teratogenic effects of ethanol manifested against the background of experimental syndrome of iron deficit in pregnant females. Mechanism of its antiteratogenic action is probably based on stimulation of cell detoxification mechanisms, increase in energy potential of cells, as well as on stabilization of structural and functional state of cell membranes.

2,2'-Dipyridyl↗

PubMedDate not supplied

[The effect of a serotonin deficiency on mammalian embryonic development].

Administration of p-chlorophenylalanine to mouse females leading to a decreased level of endogenous serotonin during the early periods of pregnancy leads either to the absence of cytokinesis at the stage of zygote or to a sharp reduction of the cleavage rate in embryos up to its complete arrest. Formation of the blastocyst is impaired, although cavitation may take place at the same time as in the control. In the latter case, the blastocysts consist of a small number of large blastomeres, and the separation into trophoblast and inner cell mass is characteristically indistinct. Decreased level of endogenous serotonin at early postimplantation stages, i.e., during active organogenesis, leads to abnormalities in the development of brain, eyes, jaws, abnormalities of the brain vessels and vascular system of other body regions, which appear as numerous hematomas and hemostasis in vessels.

Abnormalities, Multiple↗

PubMedDate not supplied

[The effect of sodium diethyldithiocarbamate on the development of organ anlagen in the mouse].

The effect of noradrenaline-depressing natrium diethyldithiocarbamate (DDK) on embryonic tissue was studied in histological preparations. Degenerative changes in cells were found in tissues, exposed to the effect on 10th day of the development. Masses of detrite appear to remove into the cavities of the organs and into the amniotic cavity of the saccus vitellinus which is the characteristic peculiarity of the degenerative changes. After effect on 9-day-year-old embryos degenerative changes were expressed in a different extent-from light in some embryos up to the massive ones in the others. Degenerative changes in giant trophoblast cells, nervous tube, eye rudiment etc were observed in the survived embryos, Prevailing location of the processes was not demonstrated. DDK manifests strong cytotoxic effect. This phenomenon is probably connected with the copper-containing enzymes depression, including those participating in noradrenaline synthesis.

Animals↗

PubMedDate not supplied

[The development of transplants of human embryonic neocortex in the anterior chamber of the rat eye].

Dorsolateral regions of embryonic human neocortex at 9-11 weeks of development were transplanted into the eye anterior chamber of rat. Significant changes, leading to the complete destruction of the original cytoarchitectonies occured during the first three days. Intensive neuroblast degeneration in cortical and interstitial zones as well as decrease of mitotically deviding cell numder were observed 3-6 hours after the transplantation. Neuroepithelial cells form rosettes in ventricular zone by the end of the first day. Rosettes turned into the centres of proliferation, mitotic activity restored to the initial level. Cells migrated out of the rosettes in radial directions. By the end of the third day almost all the cells in cortical and interstitial zones degenerated, transplant was almost completely represented by the ventricular zone cells. Number of mitotic figures exceeded the initial level almost three times, transplant was enlarged, its blood supply was absent. Since the 4-th day mitotic activity decreased, transplant underwent lymphoid infiltration and its complete destruction occured by the end of the second week.

Animals↗