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Biomedical subjects

V Delgado

Publications and source records attributed to V Delgado.

30 records · Page 2Linked to original sources

Some indicative parameters on diagnostic radiology in Spain: first dose estimations.

The Medical Physics Group at the Complutense University of Madrid has been co-ordinating, for approximately 1 year, a project on optimization of radiation protection in diagnostic radiology, in co-operation with the other states of the European Community. Exhaustive data on the subject, which offer accurate results on patient dosimetry for the different types of examination, are the final aim of the project. So far, it has been possible to analyse in detail the data from the National Institute of Health (NIH), which manages the care of about 96% of the Spanish population, plus the findings from several hospitals, outpatient centres and private clinics of the community of Madrid, which allowed us to perform the first dose estimations and to extrapolate them to the rest of Spain. The following estimations are presented: annual frequency of different examinations, their variation from 1985 to 1986, number of diagnostic rooms used for a given minimum of annual examinations, organ doses for different examinations, effective dose-equivalent, genetically significant dose for some examinations, as well as the collective dose.

Humans↗

Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes.

We compared the major polypeptides of epimastigotes and trypomastigotes of T. cruzi, by submitting total parasite lysates to electrophoresis in polyacrylamide gels (SDS-PAGE), protein staining with Coomassie brilliant blue, laser densitometry, or immunoblotting with sera derived from infected individuals (Chagas' disease). Epimastigotes and trypomastigotes displayed extensive homology, the differences being quantitative, except for a trypomastigote-specific band of Mr 75,000 which reacted with chagasic sera. Immunoblotting with chagasic sera confirmed the electrophoretic homology of epimastigotes and trypomastigotes. Upon antigenic dilution, a cluster of antigenic bands in the range of Mr 150,000 to 75,000 prevailed in the trypomastigotes, whereas the epimastigotes displayed more abundance of antigenic bands in the range of Mr 72,000 to 36,000.

Animals↗

[Sweet's syndrome in an infant].

A new case of Sweet syndrome in a six mouth old child is reported. Interesting facts with respect to the age, hematology findings, localization and residual lesions are found. The disease in the pediatric age have been reviewed.

Humans↗

[Susceptibility of Biomphalaria glabrata to Schistosoma mansoni from Venezuela and Brazil].

Schistosoma mansoni partially develops its life cycle into snails of the Family Planorbid. Biomphalaria glabrata represents an important host-intermediate. This paper reports experimental infection with miracidia vs. snail in sympatric and parapatric combination. The infection assay to sympatric combination were: BH snail (Belo Horizonte, Brasil) vs. a common geographic origin parasite, and Barbula, Carabobo State, Venezuela vs. SM Venezuela parasitic. The parapatric combination were: BH snail vs. SM; Barbula vs. BH; Caripe Monagas State, Venezuela vs. SM and Caripe vs. SM. During the study period was observed not miracid penetration. The infection percentage ranged from 88.89% in Barbula vs. SM combination to 0.00% in the Caripe vs. BH and BH vs. SM combination. We concluded which different existence of susceptibility from evaluated combination.

Animals↗

Surface morphology and characteristics of hemocytes of Biomphalaria glabrata (Pulmonata: Planorbidae) from two geographic sources.

Biomphalaria glabrata is a fresh water snail of medical importance since it is the intermediate host of the trematode Schistosoma mansoni. The internal defense system of mollusks is mostly represented by circulating elements of the hemolymph (hemocytes). The infectivity of S. mansoni to B. glabrata is determined by genetic factors and evolutionary adaptations. In the present work factors about the parasite/snail relationship were evaluated, especially those related to the morphology and characteristics of the surface of cells present in the hemolymph of two strains of B. glabrata: a strain with high susceptibility to S. mansoni (Puerto Rico, PR) and a strain with medium susceptibility (Caripe, Ca). Hemolymph was collected by cephalopodal puncture; a total and a differential count of hemocytes were done in dyed preparations and through scanning electron microscopy (SEM). Results from both strains show a high quantitative variability of the total hemocyte count. Hemocytes dyed with May-Grünwald Giemsa showed cells with a basophilic nucleus predominantly in PR (61.7%), dense and strongly basophilic in Ca (71.2%) with significant differences between them. Through SEM round cells with a corrugated surface were observed (6-10microm), hemocytes with an irregular spongy surface (12microm), others with many projections (16microm) and cells not reported in similar to erythrocytes (21microm). Hemocytic cells from both strains confirmed cytoadherence and encapsulation were confirmed in the hemocytic cells from both strains, while no differences associated to the susceptibility of the strains were observed after 2 h of parasite-hemocyte incubation.

Animals↗