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Biomedical subjects

V Brumfeld

Publications and source records attributed to V Brumfeld.

24 records · Page 2Linked to original sources
PubMedDate not supplied

Automation of laboratory instrumentation--recent achievements in the Stefan S. Nicolau Institute of Virology.

An automated system consisting of an SPF-500 spectrofluorometer interfaced to a Felix-M18 microcomputer was provided with a series of additional accessories benefitting from the inputs and outputs through the interface. An adequate set of programs for computer-controlled operations was developed. The system has allowed the performance of more than 15,000 absorption, emission and light scattering determinations. The role of interfacing to low cost computers in the automation of presently available laboratory instrumentation is discussed.

Academies and Institutes↗
PubMedDate not supplied

Light scattering from polycation--virus--oligocation mixtures.

The effect of a polycation (histone H2A) and of oligocations (spermine, spermidine and putrescine) on Sendai and influenza virus particles was investigated by light scattering measurements at 90 degrees and at various wavelengths. The method allows the study of the changes in the size and shape of virus particles in suspension.

Cations↗
PubMedDate not supplied

Polycation-induced alterations of the envelope surface of influenza and parainfluenza viruses.

Natural macromolecular polycations (protamine, histones H1, H2A, H2B and H3) caused the clustering of influenza and parainfluenza viruses into aggregates heterogeneous in size, as well as a decrease in their hemagglutinating capacity. Polycation-induced virus alterations consisted in surface pyknosis and an increase most efficient as regards the interaction with viruses.

Chemical Phenomena↗
PubMedDate not supplied

Felix-M18 microcomputer-controlled automation of spectrofluorometric measurements with applications in virology.

A system consisting of an Aminco SPF-500 spectrofluorometer interfaced to the Felix-M18 microcomputer was used in the automation of spectrofluorometric measurements with applications in virology. Programs, routines and subroutines in BASIC and in assembly language allow the operation of the spectrofluorometer and the processing of photometric data. The high efficiency of the system is illustrated by an application: the characterization of the fluorescence anisotropy value in polarized light of an influenza virus suspension preincubated with a fluorescent probe.

Computers↗