Search PubMed⌕ Search

Biomedical subjects

V Brodan

Publications and source records attributed to V Brodan.

At least 19 recordsLinked to original sources
PubMed1987

The metabolic effects of acute and chronic administration of gastrozepin.

The structure of gastrozepin (G) resembles that of tricyclic antidepressants and antihistaminic and antiserotonic cyproheptadine which are compounds with known metabolic effects. The object of this study was to test the potential action of G on the metabolism of basic nutrients, insulin and selected hormonal parameters after parenteral administration (10 and 20 mg G) and after chronic oral administration (50 mg G) during antiulcer therapy. Investigations carried out in two groups of 7 and 9 healthy volunteers, respectively, and on one group of seven patients with ulcer disease, revealed no changes in any of the blood parameters studied, nor in the excretion of catecholamines or 17-OH steroids in the urine. Our observations practically rule out any metabotropic activity of G, thereby differentiating it from tricyclic antidepressants and cyproheptadine. Isolated significant differences from the basal value seen after G and saline, can be attributed to other than pharmacological or specific mechanisms such as stress associated with the first examination, effect of fasting, individual response of the subjects, and so on.

Administration, Oral↗
PubMed1986

Effect of sleep deprivation on cholesterol metabolism and triglyceridaemia in male volunteers.

The effect of 5-day sleep deprivation (SD) on cholesterol metabolism, together with triglyceridaemia, was studied in seven healthy male volunteers. A 3-day control period was followed by 5 days (120 h) complete SD and 4 days recovery. Blood was collected at 9 a.m. and at 9 p.m. Vastus lateralis muscle biopsy was performed during the control period, on the 5th day of SD, and on day 3 of recovery. The value of muscle cholesterol was related to the non-collagen protein content. The plasma triglycerides (TG) varied in a circadian biorhythm, the amplitude of which declined gradually during SD. The morning triglyceridaemia was significantly decreased on days 3-5 of SD (35%-79% of initial values). On days 4 and 5 of SD, plasma cholesterol fell significantly to 78% and 88% of control values, respectively. The ratio of its esterified to unesterified fractions remained unchanged throughout SD. Basal activity of lecithin cholesterol acyltransferase (LCAT) showed no diurnal biorhythm; on the last 2 days of SD, LCAT activity fell significantly to 71%-80%. In contrast, the decrease in fractional esterification rate (FER) was insignificant. In the vastus lateralis muscle, total cholesterol (TC) was decreased by 40% at the end of SD, the reduction being greater for cholesterol esters (CE) (by 63%) than for free cholesterol (FC) (by 36%). The relative proportion of CE significantly decreased from an initial 14.7% to 9.2% on the last day of SD. During recovery after SD, plasma cholesterol and TG slowly returned to normal. LCAT activity and FER recovered quickly, within 48 h.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1986

Circadian rhythms of urinary sodium, potassium and some agents influencing their excretion in young borderline hypertensives.

The circadian rhythms of sodium (Na+), potassium (K+), aldosterone (ALDO), 11-deoxycorticosterone (DOC), 18-hydroxy-DOC (18-OH-DOC), cortisol (F), corticosterone (B), adrenaline (A), noradrenaline (NA), dopamine (DA), metanephrine (MN), normetanephrine (NMN), homovanillic acid (HVA), kallikrein (KE) and prostaglandins E (PGE) and F2 alpha (PGF2 alpha) excretion were studied in eight young borderline hypertensive men (BHT) and in six healthy age-matched normotensive subjects (NT) during hospitalization, after adaptation to the hospital regimen and a diet containing 135 mmol Na+ and 90 nmol K+. Urine samples were collected in 4 h periods for 24 h: 1. at normal daily activity, 2. during a 24 h bed rest. The results were evaluated by means of cosinor analysis. The circadian rhythms of ALDO, F, B, 18-OH-DOC and PGF2 alpha excretion were similar in both groups, with the exception of higher mesors of urinary ACTH-dependent corticosteroids in BHT at normal daily activity. Differences were observed in the rhythms of Na+, A, NA, DA, HVA and PGE excretion, this, however, above all during the normal daily regimen. The curve of urinary Na+ was flat in BHT due to a higher portion of Na+ excreted during the night. In the rhythms of A, NA and PGE excretion, the acrophases were shifted to the later hours in BHT, and the mesors of A and NA were also significantly higher. In contrast, DA and HVA excretion curves were flat with a lower mesor. These changes normalized, or at least mitigated, during a 24 h bed rest. The results of the study show that the circadian rhythm of Na+ excretion are disturbed already in the initial stage of hypertension. The likely cause is the exaggerated rise in adrenergic activity in the kidney during the day, induced by orthostasis and the concomitant physical (and mental?) activity, which is inadequately compensated for by the activation of natriuretic mechanisms.

Adolescent↗
PubMed1985

Changes in the EEG spectrum at a two-week intensive endurance training.

Marathon runners in the course of a two-week endurance training, (i.e. in the beginning, after one and after two weeks) and processed by the off-line method of fast spectral analysis. Simultaneously biochemical parameters were monitored which indicated after one week the development of the so called "pseudouremic syndrome of top athletes" of not too great intensity. In the second week of the experiment therapeutic intervention was made in six subjects, i.e. reduced protein supply, greater amount of glucose, application of Ca-lactate and higher amount of liquids. This intervention eliminated entirely the signs of pseudouremic syndrome. The EEG showed in both groups a decrease in theta activity (4-6 Hz) and an increase of the slow alpha component and subtheta activity (6-8 Hz), with no basic difference between the intervened and control group. Thus the changes may be rather attributed to fatigue and perhaps to a poorer supply of the CNS with oxygen and glucose.

Adolescent↗
PubMed1984

Importance of investigation of myocardial metabolites in diagnosis and evaluation of treatment of ischaemic heart disease.

Myocardial metabolism was examined in 114 patients with ischaemic heart disease. In selected subgroups, possibilities were detected of using this method in clinical cardiological diagnostics for determining or defining more precisely the diagnosis of ischaemic heart disease, and for objective evaluation of conservative or surgical therapeutic approach in ischaemic heart disease.

Adult↗
PubMed1983

The effect of five days' fasting on skeletal muscle enzymes in obese men.

The effect of 120 hours' (five days) fasting on the activity of some enzymes of energy metabolism in skeletal muscles was investigated in six obese young men. The results revealed a significant decline in the activity of the following enzymes: triosophosphate dehydrogenase (by 20%), glycerol-3-phosphate dehydrogenase (by 24%), lactate dehydrogenase (by 13%), citrate synthase (by 20%), hydroxyacyl-CoA dehydrogenase (by 40%), while the hexokinase and malate dehydrogenase activities were not significantly altered. Contrary to muscles of non-obese healthy men (Vondra, Bass, Brodan, Kuhn, Andĕl, Veselková and Vítek 1982), a smaller decline of activities of the investigated enzymes occurred together with a paradoxical change of the enzyme pattern, namely a predominance of carbohydrate catabolism and a decline of the role of fatty acids in muscle energy metabolism.

3-Hydroxyacyl CoA Dehydrogenases↗