Cell sorting by continuous flow cytometry.
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Journal Article↗
Biomedical subjects
V Britten
Publications and source records attributed to V Britten.
Immune suppression to Trypanosoma cruzi antigens is associated with infection but not immunisation.
Immune responses to Trypanosoma cruzi antigens in infected (C57BL/6 X C3H)F1 hybrid mice were followed throughout the acute infection and into the chronic phase, and compared to immune response kinetics of immunised, but uninfected, mice of the same strain. Anti T. cruzi antibody was detected by day 10 in infected animals and by day 5 in antigen sensitised mice. Animals sensitised with T. cruzi antigens showed strong delayed type hypersensitivity (DTH) responses to T. cruzi antigens. Infected animals were non-responsive during acute infection and immediately after resolution of parasitaemia, but showed similar responses to immunised animals during the chronic phase. The cell mediated immune response to T. cruzi antigens was assessed in vitro lymphocyte transformation tests. Antigen-sensitised mice were responsive to antigen at all time points. Infected animals showed reduced responses during acute infection but attained levels similar to antigen-sensitised mice around 60 days post infection. The results suggest immune suppression during the acute phase is related to infection, but that, unlike other trypanosome infections, suppressor cell-inducing antigens are not present in the T. cruzi antigen preparations tested.
Acute Disease↗
Isolation and characterisation of human T-cell lines from a patient with Chagas' disease.
Short-term T-cell lines reactive to different Trypanosoma cruzi antigens were isolated from a patient with Chagas' disease. These T-cell lines were analysed phenotypically with monoclonal antibodies defining pan-T (T11), helper (T4), or cytotoxic/suppressor epitopes by the use of a continuous-flow microfluorimeter. One cell line, 5C3, was obtained from T-cell blasts reactive to formaldehyde-fixed amastigotes (the intracellular stage of the parasite) plated at limiting dilution (3.3 cells/well). This line was shown to be T11 and T4 positive, to respond to specific antigen in an HLA-DR restricted manner, and to produce interleukin-2 under similar growth conditions.
Antibodies, Monoclonal↗
Immune response to South American trypanosomiasis and its relationship to Chagas' disease.
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Animals↗
In vitro activation of natural killer-like cytotoxicity by specifically in vivo primed T-helper lymphocytes in the rat.
The interaction between the rat non-cytotoxic T lymphocyte subset, which is marked by the W3/25 monoclonal antibody, and natural killer cells was investigated. Specifically in vivo primed lymph node cells were restimulated in vitro with the priming antigen and co-cultured with a source of natural killer cells and their precursors. Cytotoxic activity, generated during a 4 day incubation period, was assessed by lysis of a rat natural killer cell-sensitive tumour target cell line, y3Ag123. This cytotoxic activity was more fully described as natural killer cell cytotoxicity on the basis of target cell specificity, using a range of natural killer cell-susceptible and -resistant targets. The W3/25-positive T cell, separated from the in vivo primed lymph node cells by nylon wool column elution, antibody labelling and sorting on the fluorescence-activated cell sorter, was shown to be necessary to stimulate the generation of this activity. W3/25-negative cells were not active in this respect. The activation was shown to be mediated via lymphokine(s), probably interleukin-2, present in concanavalin A-induced lymphocyte culture supernatants. These supernatants could be used to substitute for in vivo primed, restimulated W3/25-positive lymph node cells in activating natural killer cell cytotoxicity from normal spleen cells. Nylon wool column-eluted spleen cells, activated in vitro with conditioned medium were separated into OX8-positive and OX8-negative subsets using the fluorescence-activated cell sorter. The distribution of cytotoxic activity related to that of freshly derived rat natural killer cells.
Animals↗
Natural killer cell activity and response to phytohaemagglutinin compared with the histological diagnosis of patients with transitional cell carcinoma of the bladder.
In vitro tests of immune reactivity in patients with transitional cell carcinoma (TCC) of the urinary bladder were compared with the clinical and histological diagnosis. Response of peripheral blood lymphocytes to the mitogen phytohaemagglutinin (PHA) tended to be reduced in patients with more advanced disease, although the difference between patients with advanced disease and those with early disease or controls was not statistically significant (P greater than 0.05). Assessment of natural killer (NK) cell activity in the same patients showed no change in levels of reactivity at any stage of the disease. There was no difference between the NK cell activity of patients with TCC of the bladder and normal controls.
Aged↗
Changes in immune reactivity during growth of an adenovirus 12-induced transplantable tumour in CBA mice.
Early suppression, followed by a period of enhancement and finally, suppression, was seen when the spleen cell response to T and B cell mitogens was monitored during growth of an adenovirus 12-induced tumour in CBA black mice. The macrophage content of the tumour changed with time and these fluctuations correlated with the ability of tumour tissue extracts to enhance the normal spleen cell response to mitogen.
Adenoviruses, Human↗