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Biomedical subjects

V Bohr

Publications and source records attributed to V Bohr.

25 records · Page 2Linked to original sources
PubMed1978

In vitro crosslinking of DNA by 8-methoxypsoralen visualized by electron microscopy.

By electron microscopic visualisation of totally denatured DNA, we have detected photochemically induced 8-methoxypsoralen crosslinks in vitro after irradiation at 360 nm. The amount of crosslinks was expressed as the percentage of DNA length which was kept in double-stranded appearance by closely situated crosslinks. This percentage correlated well with irradiation time, irradiation intensity, and the concentration of 8-methoxypsoralen. These parameters have also been correlated with the mean size and the size distribution of non-crosslinked regions of DNA, so called bubbles. For a comparison with another psoralen type, we have carried out a similar set of experiments using 4,5,8-trimethylpsoralen.

Coumarins↗
PubMed1978

Effects of purine riboside on nucleic acid synthesis in ascites cells.

Purine riboside (nebularine, 9-beta-ribofuranosylpurine) is a naturally occurring base analog which closely resembles adenosine. It inhibits carcinogenic growth. Purine riboside strongly inhibits RNA and DNA synthesis in different cancer ascites cells. Gel electrophoretic analysis of RNA synthesis in vivo in the presence of purine riboside shows the ribosomal components to be inhibited the most. A method for assaying purine riboside or its phosphates intracellularly has been devised, and by using this it has been shown that purine riboside is extensively phosphorylated in the cells. The triphosphate derivative of purine riboside has been isolated and tested in the Escherichia coli RNA polymerase assay. It appears not to be incorporated into this type of RNA and to competitively inhibit this reaction with regard to ATP.

Animals↗
PubMed1978

DNA interstrand crosslinks in normal human skin visualized by electron microscopy.

Normal human skin was investigated for DNA interstrand crosslinks by a denaturation electron microscopic method. Skin biopsy materials from the thighs of 8 healthy volunteers were separated into epidermis and dermis. DNA from each layer was isolated, purified and studied in the electron microscope. The presence of DNA interstrand crosslinks in normal human skin was demonstrated. The number of crosslinked DNA molecules and the density of crosslinking within the molecules were determined. Crosslinks were found in 0.8% of the scored molecules and were located almost exclusively in epidermal DNA. The intramolecular density of crosslinking showed a wide range of variation in each volunteer and between the volunteers.

Adult↗
PubMedDate not supplied

Artificial ventilation and prognostic factors in bacterial meningitis.

The use of artificial ventilation in patients with bacterial meningitis was increased from 8.0% of 176 patients admitted in 1966-1968 to 31.5% of 162 patients admitted in 1975-1976. The therapeutic regimen was otherwise unchanged. The fatality rate decreased from 14.2% in the first period to 8.6% in the second, whereas the rate of neurological sequelae increased from 11.3% to 16.9%. A linear logistic model analysis was applied to correct for the influence of factors of known prognostic importance in the two periods, e. g. age, level of consciousness at admission, mode of admission and etiology. The analysis showed a significant 50% reduction in fatality rate (p = 0.05), whereas the corrected rate of neurological sequelae appeared similar in the two periods. Our results suggest that an increased use of respirator treatment may improve the prognosis in bacterial meningitis.

Humans↗
PubMedDate not supplied

Mortality in the years following bacterial meningitis.

During the years 1966-1976, 875 patients were treated for bacterial meningitis at the University Clinic for Infectious Diseases, Copenhagen. By about January 1, 1980, all 782 surviving patients had been traced. 87 had died in the observation period of four to 15 years. Mortality in the years following meningitis was studied by means of a comparison with the expected mortality in a matched normal population, using a computer program for the determination of late excess mortality. Late excess mortality was significantly increased during the first two years following discharge after meningitis and was of the same magnitude in the major etiological groups. The cumulative five-year late excess mortality rate was higher in the group of patients between 30 and 60 years, in those transferred from other hospitals, in those in coma or somnolence on admission and in those developing convulsions during hospitalization. In the group of patients aged 30 to 60 years, 11 patients died during the first two years after discharge. In nine of these cases, the main cause or the concomitant causes of death were conditions predisposing to infections or bacterial meningitis. The frequency of the causes of death in the 87 patients who died was not significantly different from that among the general Danish population.

Adolescent↗