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Biomedical subjects

V Amato Neto

Publications and source records attributed to V Amato Neto.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[Eventual effect of artemisinine on the experimental infection of mice by Toxoplasma gondii].

Because of taxonomic similarities to previous data found in the literature, and with the aim of improving treatment of toxoplasmosis, we considered it of interest to assay artemisinin, an effective anti-malarial agent, for the treatment of experimental infection in mice by Toxoplasma gondii. Different amounts of the anti-parasitic agent were administered, including after previous contact in vitro with toxoplasma and stimulated macrophage production. With the methodology used artemisinin was not effective. Suggestions for further studies are made.

Animals↗
PubMedDate not supplied

[Xenodiagnosis in Chagas disease: influence of the triatominae sex].

We evaluated, comparatively, the performance of female and male Triatoma infestans in the 5th instar of development, in xenodiagnosis for Chagas' disease. Xenodiagnosis were done in 40 patients with chronic infectans. For each diagnosis 20 nymphs female and 20 nymphs males were used, which were weighed immediately before and after the meal. Intestinal contents were examined about 20, 30, 60 and 90 days after the xenodiagnosis application. The females and males ingested the mean of 230 and 210 mg of blood, respectively. The results revealed positivity of 15 (37.5%) out of 40 xenodiagnosis, but any of the positive tests show 100% of the nymphs, female and/or male infected by T. cruzi. The females ingested significantly more blood than the males; this is coincidental with the higher female positivation to T. cruzi here observed. Our results suggest that the female nymphs seem able to increase the sensibility of the xenodiagnosis for chronic patients with Chagas' disease being necessary further studies are necessary to confirm this hypothesis.

Animals↗
PubMedDate not supplied

[The use of sodium azide as a fecal preservative for studying the eggs of Schistosoma mansoni and of other helminths by the Kato-Katz method: a study in an endemic area].

In two aliquots of 208 samples of stool, tests have been made by the Kato-Katz method, one being made after the use of 0.2mg of sodic azide for 200mg, and the other without the aforesaid conservant. The resulted percentages with or without the conservant were, respectively, for Ancylostomideos: 12.5 and 25.9; for Ascaris lumbricoides: 71.6 and 72.5; for Schistosoma mansoni: 7.6 and 17.7, and for Trichuris trichiura: 86 and 85. The count of the eggs with and without the conservant was, respectively, 264 and 539 for Ancylostomideos, 13186 and 33751 for A. lumbricoides, 55.5 and 63.5 for S. mansoni, and 1345 and 2068 for T. trichiura. The authors did not confirm the advantage of using sodic azide for study in endemic areas. They suggest that the exsiccation of the stool and the low intensity of infections can explain the unfavourable results of the present clinical trial.

Animals↗
PubMedDate not supplied

[The evaluation of the effect of formol and sodium hypochlorite on the demonstration of Cryptosporidium oocysts in feces by Heine's method].

Cryptosporidium oocysts were searched by Heine's method in stools of nine calves with cryptosporidiosis after stool treatment with two disinfectants, 10% paraformaldehyde solution and 14.5% sodium hypochlorite solution. After 30 minutes exposition to sodium hypochlorite solution oocysts became non refractile and acquired a reddish tinge, making their identification difficult. No morphological alterations occurred in oocysts after paraformaldehyde treatment. We recommend paraformaldehyde at 10% concentration as means of human immunodeficiency virus (HIV) inactivation for routine use in stool examinations and therefore making safer those type of procedures for laboratory personnel, when using Heine's method.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[The use of a silicone T tube for the treatment of a case of American mucocutaneous leishmaniasis with tracheomalacia].

The authors report a case of mucosal leishmaniasis, in which tracheomalacia and respiratory insufficiency occurred. The nature of the illness, the patient's general condition and the great extension of the affected tracheal segment made segmental resection and anastomosis inviable. The silicone T tube, which proved itself useful, was the choice, thus motivating this report of the procedure.

Chronic Disease↗
PubMedDate not supplied

[The characteristics of Trypanosoma cruzi strains isolated from patients who have undergone a heart transplant].

Three strains of Trypanosoma cruzi were isolated from Chagas' disease patients transplanted for heart failure, after cardiac transplantation, and were studied in an experimental model of Chagas' disease, in mice, with evaluation of parasitic load, mortality and extension of inflammatory infiltrates in the heart. These parameters were compared with the standard strain Y. The strains had differences in the studied parameters, but there was no clear relationship between those and post-transplant evolution of the patients. Probably the clinical response is multifactorial and derives only in part from biological characteristics of the infecting T. cruzi strain, as measured in our model.

Adolescent↗
PubMedDate not supplied

[The treatment of cryptosporidiosis in AIDS patients using paromomycin].

The authors treated with paromomycin 25 patients, with AIDS and cryptosporidiosis. The drug was given orally in a doses of 500 mg qid, for a period of 14 days. Tolerance was good, with just two cases of mild side-effects. Clinical improvement was obtained in 19 (76%) patients. Parasitological cure, however, occurred only in a low percentage (25%). In some cases where initial success was observed, recrudescence occurred after some weeks or few months, but with retreatment again clinical improvement was obtained. Even if it does not lead to frequent parasite eradication, the good clinical results and tolerance permit us to consider paromomycin one of the few drugs effective for the treatment of cryptosporidial diarrhea in AIDS patients. Studies with maintainance therapy are indicated.

AIDS-Related Opportunistic Infections↗