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Biomedical subjects

V Amato Neto

Publications and source records attributed to V Amato Neto.

At least 181 records · Page 10Linked to original sources
PubMedDate not supplied

[Isolation of oocysts of Cryptosporidium sp. in loose stools of patients with AIDS and of immunocompetent children and adults in São Paulo, Brazil].

On the aim of knowing better the prevalence of cryptosporidiosis in Brazil, we performed a survey, in São Paulo, in loose stools of patients with AIDS and immunocompetent children and adults. Using strict methods oocysts of Cryptosporidium sp. were found in 10.4% of the samples; higher rates were observed for patients with AIDS, and children attending day-care centers. The result observed may be considered important and indicate the need to include the search of this organism in routine stool examination.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[Evaluation of the therapeutic activity of fluconazole in acute experimental infection caused by Trypanosoma cruzi].

On hand of information that fluconazole can inhibit Trypanosoma cruzi, infection, an experimental investigation in acutely infected mice treated with 200 mg/kg daily of this drug during one month was carried out. Parasitemia patency, blood culture, sub-inoculation and histologic examination were the parameters used to evaluate the potential therapeutic activity of the drug. The results did not confirm the hypothesis, but it would be desirable to conduct new trials with this and other imidazole compounds.

Acute Disease↗
PubMedDate not supplied

[The evaluation of the effect of formol in the detection of Cryptosporidium sp. oocysts in feces by the methods of Sheater and of the Coprotest].

Frequently it is necessary to search for oocysts of Cryptosporidium sp. in the stools potentially contaminated with human immunodeficiency virus (HIV). For the protection of the laboratory personal handling fecal material formalin has been considered useful. However, this disinfectant has not been evaluated when the stool examinations were performed with the technique of flotation-centrifugation in concentrated solution of sugar and with that of "Coprotest". Our experience indicates that the use of formalin may interfere with the results when the first of these methods is used, but it does not alter the results of the second one. This information is of importance for the future assistential and scientific studies.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[The early diagnosis of leptospirosis by demonstrating antigens via an immunohistochemical exam of the hamstring muscle].

Establishing an early and certain diagnosis of leptospirosis has been a difficult task. So, we analysed the histopathological alterations of the gastrocnemius muscle, and studied, for the first time, the usefulness of the immune histochemical method of peroxidase-antiperoxidase for the demonstration of the spirochete and of its products in this tissue. Histopathological observations have shown a picture of myositis, characterized by interstitial inflammatory infiltrate and necrotic-degenerative abnormalities of muscle fibers. The lesions were considered minimal in 69.45% of the patients, moderate in 19.45%, severe in 5.55% and absent in the remaining. On the other hand, the immune histochemical method identified the etiology in 94.45% what was considered very expressive.

Adolescent↗
PubMedDate not supplied

[Frequency of malaria relapse due to Plasmodium vivax in a non-endemic region (São Paulo, Brazil)].

Very few well-established information is available about the frequency and timeliness of relapses in cases of Plasmodium vivax malaria acquired in Brazil. So, we analysed a series of correctly treated patients observed out of endemic areas. The rate of relapses seen in São Paulo, which may represent that of the parasitosis in the whole country, was high, ranging from 7.5% to 24.5%, and early in most cases, i.e. appearing by three months, what anticipates a high endemicity.

Brazil↗
PubMedDate not supplied

[Attempts to culturing triatominae in the laboratory using "in vitro" feeding].

In order to simplify breeding of triatominae in the laboratory, for performing xenodiagnosis and other biologic studies, we tried to feed the insects "in vitro" with citrated or defibrinated blood from commercially abated chicken. Two types of efficacy observations were carried out with Triatoma infestans: a) analysis of the chaining of successive nymphal stages, viability of satisfactory matching, fertile oviposition and adequate reproduction; b) assessment of viability and infectivity of Trypanosoma cruzi in the insects. As a conclusion, it became evident that, despite operational easiness, the objectives were not achieved, since the classical procedure used as the control, was always superior.

Animals↗
PubMedDate not supplied

[New process for screening of drugs in experimental infection with Trypanosoma cruzi].

We propose a screening process for detection of therapeutic activity of drugs against experimental infection with Trypanosoma cruzi. It is based on the use of infected triatominae that are fed on mice which have received the study drug. Blood meals are made at different time schedule in order to adapt with serum drug levels. The digestive tube of the hemiptera will, thus, work as a suitable structure for examination. In a initial observation, benzonidazole was used, and was shown to be only partially active at least in the conditions of this new procedure.

Animals↗
PubMedDate not supplied

[Postoperative tetanus: report of a case and prophylaxis].

We describe a case of a patient presenting tetanus after mastectomy with postoperative complications. Risk factors and prophylaxis of this disease after surgery are discussed, emphasizing the need for vaccine protection and evaluation of all patients before any kind of surgical procedure.

Aged↗
PubMedDate not supplied

[Treatment of ascaridiasis with garlic (Allium sativum)].

In Brazil, there has been growing interest on alternative therapeutic approaches, as shown by asi increase of homeopathic prescriptions, and use of acupuncture and of the so called natural products. This study evaluated the usefulness of garlic (Allium sativum) for the treatment of ascaridiasis, trying to confirm the popular belief on this matter. Eight grams daily, as an infusion were used during five days, in children known to be infected with Ascaris lumbricoides. Little efficacy was observed, and this fact enhances the importance of not accepting propositions not based on adequately conducted studies.

Adolescent↗
PubMedDate not supplied

[Effect of cyclosporin and prednisone on the acute phase of experimental infection of mice by Trypanosoma cruzi].

The relations between immunosuppression and human or experimental infection by Trypanosoma cruzi have recently been the subject of great interest, specially because of the wider use of drugs acting on immunity and of organ transplantation programs, now in frank expansion. The use of cyclosporin turned necessary to know adequately its interactions with T. cruzi parasitosis, and this has been the objective of the present study, regarding the acute phase in mice, in comparison to the use of prednisone. It was verified that cyclosporin, alone or associated to the corticosteroids, promotes and enhancement of the parasitosis, as shown by the assessment of parasitemia, the amount of amastigote forms in the heart and the severity of cardiac inflammation. Such observations may be useful in clinical practice, calling the attention for necessity of a strict control of such cases an eventually the use of an antiparasitic agent.

Acute Disease↗
PubMedDate not supplied

[Evaluation of the therapeutic effect of albendazole on experimental and human infections by Hymenolepis nana].

With the objective of knowing adequately the spectrum of activity of albendazole against intestinal helminthiases, we made observations regarding hymenolepiasis caused by Hymenolepis nana. Two series of investigations were carried out: a) treatment of mice with single doses of either 25 or 50 mg/kg, repeated after ten days, using as controls animals treated with 25 mg/kg of praziquantel or not treated with any antiparasitic drugs; b) treatment of adults and children with 400 mg daily for three consecutive days, repeated after ten days.

Adolescent↗
PubMedDate not supplied

[Isolation of Toxoplasma gondii from the saliva of patients with acquired immunodeficiency syndrome (AIDS)].

Toxoplasma gondii has been found in saliva of patients with toxoplasmosis. We tried to isolate parasites in saliva in patients with AIDS. As it is known, the finding of antitoxoplasma antibodies is not useful for diagnosis of cerebral abscess in toxoplasmosis occurring in AIDS patients in Brazil where 90% or more adults have serological evidence of past infection with Toxoplasma gondii. In 26 patients with AIDS, including six with tomographical evidence suggesting cerebral abscess caused by Toxoplasma gondii, no parasites were found in the saliva by the use of the mouse inoculation model. This trial that the search for Toxoplasma gondii in the saliva is not an alternative method for the diagnosis of toxoplasmosis in patients with AIDS.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

[The possible therapeutic effect of deferoxamine in experimental infections of mice by Plasmodium berghei].

Deferoxamine is a compound with iron chelating properties. Body depletion of this mineral, according to some authors, might influence the metabolism of plasmodia that thrive in the erythrocytes. In order to verify this possibility, we administered daily doses of 300 or 1.000 mg/kg of the compound, for five days and again 15 days, to mice infected with Plasmodium berghei. The parameters used to check the activity of deferoxamine were mortality of the animals and the count of blood parasites. The results showed a progressive increase of mortality and of the parasitemia in all animals, without differences in relation to the controls. So, at least on hand of the present study, the mentioned substance cannot be considered useful for the treatment of malaria.

Animals↗
PubMedDate not supplied

[Therapeutic effect of benzonidazole in mice infected by Trypanosoma cruzi and immunodepressed by the combination cyclosporin and prednisone].

Because the organ transplantation is currently involving patients with Chagas' disease, we evaluated the therapeutic activity of benzonidazole in mice immunosuppressed by cyclosporine and prednisone. The purpose was to obtain further knowledge regarding the infection with Trypanosoma cruzi when the immune system is depressed. It was verified that benzonidazole, at least in the conditions of the present study, decreased the severity of the parasitic component of the infection and of the inflammatory process; it has not protected the animals, that died at the same proportion in the treated and non-treated series.

Animals↗