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Biomedical subjects

U Torelli

Publications and source records attributed to U Torelli.

At least 73 records · Page 4Linked to original sources
PubMed1977

Lymphoid blastic crisis at the onset of chronic granulocytic leukemia: report of two cases.

Two patients with a typical hematologic pattern of acute lymphatic leukemia were brought into complete remission by treatment. A few weeks later they developed a typical peripheral and bone marrow pattern of chronic granulocytic leukemia, with Philadelphia chromosome and very low leukocyte alkaline phosphatase. These cases, along with other findings recently reported in the literature, support the possibility of a previously unrecognized relationship between lymphoblastic cell populations and chronic granulocytic leukemia.

Adult↗
PubMed1977

Immunological assay of double-helical segments in RNA fractions of different molecular size extracted from acute myeloid leukemia blast cells.

Whole-cell RNA, extracted from acute myeloid leukemia blast cells, was fractionated by sedimentation through sucrose gradients. The proportion of double-helical segments present in each fraction was then determined by a quantitative microcomplement fixation assay that specifically measures double-helical RNA. Sizable amounts of double-helical segments were detected in all fractions of cellular RNA corresponding to S values higher than approximately 20. In all cell populations examined the highest proportion of double-helical segments was found in RNA fractions sedimenting faster then the 45 S ribosomal precursors RNA, i.e., in fractions including only heterogeneous nuclear RNA.

Antibody Specificity↗
PubMed1976

Accumulation of giant heterogeneous RNA molecules in acute myeloid leukemia blast cells.

Time course and "chase" experiments showed that, after incubation of acute myeloid leukemia blast cells with a labeled RNA precursor, a large proportion of radioactivity remained associated with RNA molecules larger than 45 S even after several hr. Double-labeling experiments with [5-3H]uridine and [methyl-14C]methionine indicated that unmethylated giant heterogeneous RNA larger than 45 S is processed much more slowly than the 45 S ribosomal precursor, so that relatively large amounts of fairly stable RNA of the former class accumulate in the cell. The measurement of labeled giant heterogeneous RNA molecules bound to polyuridylate-fiberglass filters showed that molecules carrying polyadenylate segments seemingly turn over faster than those lacking polyadenylate.

Dactinomycin↗
PubMed1975

Characteristics of heterogeneous nuclear RNA in normal small lymphocytes and in acute leukemia blast cells. An outline.

In this short review of the characteristics of heterogeneous nuclear RNA of normal small lymphocytes and of leukemic blast cells the author points out that the average life time of the molecules of this RNA class in both types of cell is much longer than that so far reported for rapidly proliferating cells. In both types of cell the newly synthesized RNA includes rapidly hybridizing sequences. There is evidence, although circumstantial, that in leukemic cells these sequences are markedly different from that of normal lymphocytes. In both normal lymphocytes and leukemic blast cells the rapidly hybridizing RNA sequences are in part involved in secondary structure. In fact, a significant fraction of the hnRNA appears in RNase-resistant form with double-stranded properties. About one fifth of the hnRNA molecules of normal lymphocytes carries poly(A) segments, whereas this proportion is markedly higher in leukemic cells.

Base Sequence↗