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Biomedical subjects

U Tillmann

Publications and source records attributed to U Tillmann.

9 recordsLinked to original sources
PubMed1992

Cloning and characterization of a mouse cDNA encoding a cytoplasmic protein-tyrosine-phosphatase.

A mouse cDNA encoding a non-receptor-type phosphotyrosine phosphatase (PTP; EC 3.1.3.48) has been isolated. The 1570-base-pair cDNA contains a single open reading frame that predicts a 382-amino acid protein with Mr 44,640. The nucleic acid and amino acid sequences are homologous to those of a previously described human T-cell PTP [Cool, D. E., Tonks, N. K., Charbonneau, H., Walsh, K. A., Fischer, E. H. & Krebs, E. G. (1989) Proc. Natl. Acad. Sci. USA 86, 5257-5261]; however, the mouse and human 3' sequences diverge and predict markedly different protein carboxyl termini. The mouse PTP gene is expressed as a 1.9-kilobase message in several stages of murine embryonic development and in a variety of adult tissues. An additional 1.3-kilobase message was found to be expressed specifically in testes. Finally, we report the isolation of a human T-cell PTP cDNA containing a 3' end sequence homologous to the mouse PTP.

Age Factors↗
PubMed1989

cDNA clones of the auxin-binding protein from corn coleoptiles (Zea mays L.): isolation and characterization by immunological methods.

An auxin-binding protein (ABP) cDNA clone was selected from a lambda gt11 cDNA library from corn coleoptiles with highly purified IgGanti ABP. The sequence of 794 bp contains an open reading frame (ORF) of 603 bp, coding for a 22 kd protein. There are indications of a signal peptide of 38 amino acids (von Heijne, G. 1983, Eur. J. Biochem., 133, 17-21). A N-glycosylation site can be deduced and a C-terminal KDEL amino acid sequence is detected. An EcoRI fragment containing the beginning portion of the cDNA with about three quarters of the ORF was used to select cDNA clones from an independently produced lambda gt11 cDNA library of corn coleoptiles. Northern blot analysis with in vitro transcribed biotinylated RNA showed a single band of not more than 850 bases. The full-length in vitro transcript directed the in vitro synthesis of a protein which is precipitated by IgGanti ABP. Rabbit antibodies raised against a fusion protein detect the ABP as a double band on Western blots. Only the smaller of the two ABP bands is labeled by two different KDEL-specific IgG preparations.

Base Sequence↗
PubMed1989

[Surgically relevant complications in percutaneous transluminal angioplasty].

Seven significant surgical complications in 118 patients after PTA-treatment of the lower extremities are presented. In two cases the puncture site in the vessel had to be sutured, in three patients we had to perform a femoro-popliteal bypass, once a TEA with profundaplasty and in another case an embolectomy of the trifurcation. Our experience reveals that with the indication for PTA, the rate of significant complications and their surgical consequences has to be considered too. A close cooperation between Radiologist, Angiologist and Surgeon is of importance.

Angioplasty, Balloon↗
PubMed1979

[Pneumonitis and fibrosis after primary radiotherapy and after combined treatment with chemotherapy in bronchogenic carcinomas].

36 patients with bronchial carcinoma treated in a randomized study were investigated for late reactions of lung tissue after radiotherapy combined with chemotherapy. The onset and extent of the lesions depended on the primary therapy. In the group of patients who received primary chemotherapy the first signs of radiation pneumonitis and fibrosis were seen earlier than in the group who underwent primary radiotherapy (70 days and 120 days respectively, and 170 days and 237 days respectively). The shrinkage of the lung tissue was greater after primary chemotherapy. However, secondary pneumonia in the irradiated lung volume occurred less frequently in the group who underwent chemotherapy first.

Carcinoma, Bronchogenic↗
PubMed1979

[Computer tomography in acute pancreatitis (author's transl)].

The computer tomographic appearances of acute pancreatitis have been analysed 33 patients. It is usually a simple matter to distinguish between the acute, oedematous pancreatitis, which can be treated conservatively, and the suppurative pancreatitis with haemorrhagic and necrotising features, which require surgical exploration.

Absorptiometry, Photon↗
PubMed1979

Pain in peripheral arteriography-a comparison of a low osmolality contrast medium with a conventional compound.

The pain reaction to a new low osmolality contrast agent, sodium-methyl-glucamine-ioxaglate, in comparison to the conventional compound methylglucamine-ioxitalamate was tested and statistically evaluated in 12 patients using the visual analogue scale method. The intensity of pain experienced was significantly smaller following intra-arterial injection of the compound of low osmolality as compared to the conventional contrast agent.

Adult↗