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Biomedical subjects

U Stoll

Publications and source records attributed to U Stoll.

15 recordsLinked to original sources
PubMed1996

HPRT mutations in V79 Chinese hamster cells induced by accelerated Ni, Au and Pb ions.

Mutation induction by accelerated heavy ions to 6-TG resistance (HPRT system) in V79 Chinese hamster cells was investigated with Ni (6-630 Me V/u), Au (2.2, 8.7 Me V/u) and Pb ions (11.6-980 Me V/u) corresponding to a LET range between 180 and 12895 ke V/microns. Most experiments could only be performed once due to technical limitations using accelerator beam times. Survival curves were exponential, mutation induction curves linear with fluence. From their slopes inactivation- and mutation-induction cross-sections were derived. If they are plotted versus LET, single, ion-specific curves are obtained. It is shown that other parameters like ion energy and effective charge play an important role. In the case of Au and Pb ions the cross-sections follow a common line, since these ions have nearly the same atomic weight, so that they should have similar spatial ionization patterns in matter at the same energies. Calculated RBEs were higher for mutation induction than for killing for all LETs.

Animals↗
PubMed1995

Induction of HPRT- mutants in Chinese hamster V79 cells after heavy ion exposure.

The induction of resistance to 6-thioguanine by heavy ion exposure was investigated with various accelerated ions (oxygen-uranium) up to linear energy transfer (LET) values of about 15,000 keV/microns. Survival curves are exponential with fluence; mutation induction shows a linear dependence. Cross-sections (sigma i: inactivation, sigma m: mutation) were derived from the respective slopes. Generally, sigma i rises over the whole LET range, but separates into different declining curves for single ions with LET values above 200 keV/microns. Similar behaviour is seen for sigma m. The new SIS facility at GSI, Darmstadt, makes it possible to study the effects of ions with the same LET but very different energies and track structures. Experiments using nickel and oxygen ions (up to 400 MeV/u) showed that inactivation cross-sections do not depend very much on track structure, i.e. similar values are found with different ions at the same LET. This is not the case for mutation induction, where very energetic ions display considerably smaller induction cross-sections, compared with low-energy ions of identical LET. Preliminary analyses using the polymerase chain reaction (PCR) demonstrate that even heavy ions cause "small alterations" (small deletions or base changes). The proportion of the total deletions seems to increase with LET.

Animals↗
PubMed1995

Killing and mutation of Chinese hamster V79 cells exposed to accelerated oxygen and neon ions.

Mutation induction by accelerated heavy ions to 6-thioguanine resistance (HPRT system) in Chinese hamster V79 cells was investigated using oxygen and neon ions with energies between 1.9 and 400 MeV/mu, corresponding to LET values between 18 and 754 keV/microns, respectively. Because of technical limitations most experiments could be performed only once. Inactivation and mutation induction cross sections, sigma i and sigma m, were obtained from the slopes of the exponential survival and the linear mutation induction curves, respectively. Both parameters increased with LET up to about 200 keV/microns, where the curves separated for the two types of ions. Calculated RBEs were higher for mutation induction than for killing for all LET values.

Animals↗
PubMed1994

Mutation induction by heavy ions.

Mutation induction by heavy ions is compared in yeast and mammalian cells. Since mutants can only be recovered in survivors the influence of inactivation cross sections has to be taken into account. It is shown that both the size of the sensitive cellular site as well as track structure play an important role. Another parameter which influences the probability of mutation induction is repair: Contrary to naive assumptions primary radiation damage does not directly lead to mutations but requires modification to reconstitute the genetic machinery so that mutants can survive. The molecular structure of mutations was analyzed after exposure to deuterons by amplification with the aid of polymerase chain reaction. The results--although preliminary--demonstrate that even with densely ionizing particles a large fraction does not carry big deletions which suggests that point mutations may also be induced by heavy ions.

Animals↗
PubMed1992

[Not Available].

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History, Ancient↗
PubMed1992

Mutation induction in mammalian cells by very heavy ions.

V79 Chinese hamster cells were exposed to heavy ions (O to U) and assayed for mutants at the HGPRT-locus by incubation in selective medium containing 6-thioguanine. The LET ranged from 300 to 18000 keV/micrometer. Mutants could be recovered from all particle radiation but the effectivity per deposited energy decreased with atomic numbers greater than 8. The results are discussed with regard to fundamental processes of cell reactions to very heavy ions and with respect to possible implications for hazard estimations.

Animals↗
PubMed1978

[Reduction of the incidence of postoperative morbidity after vaginal operations by the choice of suture material (author's transl)].

Dexon suture material is capable of lowering the postoperative morbidity. Among 142 vaginal operations for prolapse 82 were done with catgut and 60 with Dexon. In the catgut group 24.4% had a temperature of 37.5 or higher for more than one day and 15.0% in the Dexon group. Secondary vaginal bleeding occurred in 15.8% of the catgut patients and in 6.6% of the Dexon patients. The mean hospital stay was 18.8 days after operations with catgut and 14.6 days after operations with Dexon. There were less wound dehiscences than in the group operated with catgut. Dexon has higher tensile strength than catgut. It is absorbed by hydrolysis and causes less tissue reaction. Dexon shows the desirable properties of both an absorable and a nonabsorbable suture material.

Female↗