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Biomedical subjects

U Rother

Publications and source records attributed to U Rother.

At least 73 records · Page 4Linked to original sources
PubMed1981

[Prevention of antibody mediated dextran side effects by hapten inhibition in dogs (author's transl)].

The effectiveness of low molecular dextran (dextran 1) in preventing antibody mediated dextran side effects (hapten inhibition) was investigated in immunized dogs. After injection of clinical dextran (dextran 60) a rapid fall of systolic blood pressure and a decrease of leucocytes, thrombocytes and haemolytic complement activity was noted. The protective effect of a dextran 1 preinjection was clearly demonstrated; in contrast the built-in hapten injection (dextran + hapten together) was ineffective.

Animals↗
PubMed1981

The attack phase of human complement: differentiation between membrane binding and complex formation by the detection of neoantigen expression in situ. A morphometric immunoferritin study.

C5-9 neoantigen was found by morphometric immunoferritin staining on the membrane of guinea pig erythrocytes lyzed by reactive lysis C5b6 or acid-activated C5 + C6 and C7, C8, and C9. Neoantigen first appeared at the C7 step, increasing with C8, and decreasing after the addition of C9. The incubation of E with C5-C8 or C5-C9 at 0 degrees C resulted in EC5-8 without neoantigen. Reincubation of these cells after washing at 37 degrees C for 5 min led to neoantigen expression. An energy-requiring step is postulated after binding of C5-C8 and needed for C9 binding and lysis.

Animals↗
PubMed1980

Failure of IgA cold agglutinin to activate C.

No complement (C) activation was observed when IgA cold agglutinin was reacted with its antigen on RBC. Neither C-consumption (CH50) nor C1 binding (classical pathway) nor conversion of factor B or C3 (alternative pathway) could be detected. In contrast, IgM cold agglutinins under the same conditions did activate the classical pathway. If the IgA was heat aggregated it was able to activate the alternative pathway as evidenced by factor B and C3 conversion. The result is consistent with the absence of in vivo hemolysis in patients with IgA cold agglutinins.

Autoantibodies↗
PubMed1980

Effect of aspirin on complement in vivo.

Following ingestion of aspirin (1 g) complement consumption could be demonstrated in 18/20 healthy volunteers. A slight to moderate decrease of whole complement activity (CH50) and of the components C1, C4 and C2 was found. In addition, reduced levels of the C1 inactivator (C1 IA) were measured in 19/20 probands. The drop in C titers and C1 IA was only detectable within a short period of time and 30 min after ingestion of the drug the titers were usually returning to normal. In view of these findings, the possible role of complement in adverse reactions to aspirin is discussed.

Aspirin↗
PubMed1980

Effect of aspirin on the complement system in vitro.

Aspirin (acetylsalicylic acid), ASA and sodium salicylate (NaSA) activate the complement system in vitro. The activation involves the components of the classical pathway. It is, however, antibody independent. Evidence for activation are (1) consumption of C and C component activities; (2) activation of C1 evidenced by release of C1s from the macromolecular complex, and (3) C3 and C5 conversion.

Aspirin↗
PubMed1980

[Activation of the complement system with clinical dextran (Macrodex 6%) in 10 patients in a cross-over design against 0,9%-NaCl].

The possibility of activation of the complement system with clinical Dextran 60 (500 ml Macrodex 6% infusion) as compaired with 500 ml 0.9% NaCl was investigated within a one week interval in 10 volunteers of both sexes in a cross-over design. Anaphylactoid symptoms, which would indicate a release of biological activities, were not induced by Dextran 60 or 0.9% NaCl. Neither could a change in blood pressure, heart rate, in the whole hemolytic complement activity nor in the leucocyte and thrombocyte count be determined. Therefore, a clinical relevant activation of the complement system in normal persons by Macrodex 6% can not be assumed.

Anaphylaxis↗
PubMed1979

[Somatic radiation load by radiography of the temporomandibular joint].

660 exposures were performed on an Alderson phantom to determine the somatic radiation exposure values for standard examinations of the temporomandibular joint. Comparative examinations with the classic contact technique according to Parma served to make clear the results obtained. The lowest amount of surface exposure was observed in contact examinations of the temporomandibular joint. The use of a flat tube leads to a 13-fold reduction in surface exposure compared to contact examinations according to Parma. Furthermore, a heavy gradient of radiation exposure from the area of exposure to the environment was stated in using a tube (flat tube and ear tube).

Humans↗
PubMed1979

Isolation of late complement components by affinity chromatography. II. Purification of the human complement component C6.

We developed a new procedure for the rapid and gentle isolation of the human complement component C6 comparable to that described previously for C9. The procedure is based on affinity chromatography. As a first step, C6 is immunoabsorbed on insolubilized anti-C6 antibodies. These antibodies were derived from C6-defective rabbits (Freiburg strain). C6 was eluted with 3 M thiocyanate, pH 7.2, with a recovery of 15--23% of its hemolytic activity and a more than 270--fold purification. Impurities were removed in a second step by an "anti-impurity" column. The final product yielded a 12% recovery of the hemolytic activity and the purification factor was higher than 1300. The final product was homogeneous in SDS polyacrylamide and immunoelectrophoresis.

Animals↗
PubMed1978

Activation of complement by radiographic contrast media: generation of chemotactic and anaphylatoxin activities.

Iodinated radiographic contrast media such as methylglucamine diatrizoate and sodium ioglycamate activate serum complement in vitro. This was shown by a dose-, time-, and temperature-dependent decrease of total hemolytic complement activity in normal human serum, consumption of C4 and C6 activity, conversion of C3 to C3b, and generation of C5-derived chemotactic and smooth muscle contracting activity. Complement activation was achieved even in sera depleted of immunoglobulin and properdin, which may indicate that contrast media induce complement activation by mechanisms different from the classical or alternative pathway.

Anaphylatoxins↗
PubMed1978

[Clinical and radiological control examinations after cyst surgery in the permanent juvenile dentition].

The present study involves 23 patients with 25 cysts whose conditions were assessed postoperatively by means of clinical and radiological criteria. The radicular and follicular cysts were most frequently located in the anterior region of the upper jaw. With 17.4% of the total number of bone cysts, the percentage of cysts on juvenile permanent teeth is relatively low. Teeth that did not respond to irritants were the most frequent complication in the operating area. Nevertheless, the clinical and radiological findings tell against the non-vitality of these teeth.

Adolescent↗
PubMed1978

Deviated lysis: lysis of unsensitized cells by complement. V. Generation of the activity of low pH or low ionic strength.

In serum exposed to acid pH (6.4), a serum activity was generated which lyzed unsensitized erythrocytes in the presence of EDTA. It was similar to the d.l. activity found following serum activation by inulin (2). In contrast to the d.l. generation by the classical or by the alternative pathway of C activation, the generation of d.l. by acid pH did not require C4 plus C2 or C3 plus factor B resp. It was, thus, not dependent on any hitherto known pathway of C activation. A similar activity appeared when NHS was centrifuged in a sucrose gradient at low ionic strength. Physicochemical alterations of the component proteins which influence their affinity for each other are seen as the basis for the activation of the attack phase of C.

Animals↗