Suction blister device for separation of viable epidermis from dermis.
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Animals↗
Biomedical subjects
U Kiistala
Publications and source records attributed to U Kiistala.
The presence of Langerhans cells in human dermis with special reference to their potential mesenchymal origin.
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Humans↗
Ultrastructural evidence related to monoamine-storage in the axoplasm of unmyelinated nerves of human dermis.
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Amines↗
Electronmicroscopic evidence of synthetic activity in Langerhans cells of human epidermis.
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Adenosine Triphosphatases↗
Resolution of melanin granules and epidermal cell types by ultraviolet television microscopy and by high-contrast phase microscopy of electron microscopic sections.
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Histological Techniques↗
Suction blisters in the study of cellular dynamics of inflammation.
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Basophils↗
Electron microscopy of Merkel's "Tastzelle", a potential monoamine storing cell of human epidermis.
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Adult↗
Culture of adult human epidermal cells obtained from roofs of suction blisters.
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Adult↗
Quantitative analysis of epidermal lipids by thin-layer chromatography with special reference to seasonal and age variation.
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Adult↗
Epidermal lipids in Besnier's prurigo (atopic eczema).
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Adolescent↗
Dermo-epidermal separation with suction. Electron microscopic and histochemical study of initial events of blistering on human skin.
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Basement Membrane↗
Differential modulation of transforming growth factor-beta by betamethasone-17- valerate and isotretinoin: corticosteroid decreases and isotretinoin increases the level of transforming growth factor-beta in suction blister fluid.
Retinoids and glucocorticoids are known to have a potential to modulate the expression of transforming growth factor-beta (TGF-beta). We investigated the effect of oral isotretinoin (13-cis-retinoic acid) on the expression of two distinct isoforms of TGF-beta, TGF-beta1 and TGF-beta2, in suction blister fluid and serum in acne patients. We also investigated the effect of topical glucocorticoid (betamethasone-17-valerate) and age on suction blister fluid TGF-beta1 in healthy volunteers. Six weeks of isotretinoin treatment caused a statistically significant 19% increase in suction blister fluid TGF-beta1. The suction blister fluid TGF-beta2 level remained below the sensitivity level of the immunoassay in many cases. Isotretinoin did not affect the serum TGF-beta1 or TGF-beta2 level. Betamethasone-17-valerate pretreatment for 3 days twice a day caused a statistically significant 17% decrease in suction blister fluid TGF-beta1. The active form of TGF-beta1 represented 5% of the total TGF-beta1 in suction blister fluid. Our diffusion calculations suggest that all TGF-beta1 and TGF-beta2 detected in suction blister fluid have diffused from systemic circulation. The increase in suction blister fluid TGF-beta1 after isotretinoin treatment seems to be of local origin, while the decrease in suction blister fluid TGF-beta1 after glucocorticoid pretreatment seems to be due to glucocorticoid-induced vasoconstriction resulting in decreased diffusion of TGF-beta1 from the circulation. Modulation of local interstitial fluid TGF-beta1 concentration may be one mechanism by which isotretinoin and glucocorticoids mediate their effects in skin.
Acne Vulgaris↗