[Monoclonal antibodies in diagnosis of breast cancer].
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Antibodies, Monoclonal↗
Biomedical subjects
U Karsten
Publications and source records attributed to U Karsten.
[Expression of intermediate filaments in cutaneous cylindroma].
Using different monoclonal anti-cytokeratin antibodies (broad spectrum, cytokeratin 7, 8, 18, 19) and the antibody A55-A/A9 until now characterized only by immunohistology the staining of ductal structures and some cells within the lobulae of dermal cylindromata was demonstrated. In regard to the cytokeratin polypeptide patterns of the adnexes in uninvolved skin an exact histogenetic correlation by our findings is not possible, but the pattern is consistent with the conception of the glandular origin of the tumours.
Antibodies, Monoclonal↗
Distribution of (fucogalactosyl-)epitope expressing glycoconjugates in rat brain.
Glycoconjugates are known to be concentrated in plasma membranes, especially in synaptic junctions, where they subserve various functions in neural connectivity. Here we report the cellular distribution of a new monoclonal antibody recognizing (fucogalactosyl) sequences in carbohydrate structures. The most pronounced immunoreactivity was found in fibrous astrocytes, in many parts of the brain and with lower density in various neuronal elements. This points to the expression of identical carbohydrate sequences on molecules within certain glial and neuronal elements. Previous intracerebral injections of the antibody interfered with long term memory formation. Therefore, functions mediated by corresponding glycoproteins in neurons and glia cells or even neuron-glial interactions, might be relevant for information-processing.
Animals↗
Detection of cancer metastases in regional lymph nodes: comparative histological and immunohistological investigations with the broad-range anticytokeratin monoclonal antibody A45-B/B3.
A total of 113 patients with carcinomas of breast, testis, stomach and colon were examined for lymph node metastases by means of an exact case-by-case comparison by conventional histology and by immunohistochemistry using the anticytokeratin monoclonal antibody A45-B/B3. Among 891 examined lymph nodes, 90% of metastases were recognized by both methods, about 2% by histology alone, and more than 10% by immunohistochemistry alone. The method can be applied for intraoperative frozen section diagnosis.
Breast Neoplasms↗
Patterns of cytokeratins and lamins in rat liver and in rat liver cell lines as shown by immunoblotting using the monoclonal antibodies A 45-B/B3 and A 51-B/H4.
The cytokeratins and lamins of rat liver nuclei, nuclear lamina, and cytoskeleton preparations as well as of rat liver cell lines were studied by immunoblotting using the monoclonal broad-range cytokeratin antibodies A 45-B/B3 and A 51-B/H4. A 45-B/B3 is bound to 64-, 58-, 55-, 52.5-, 49-, 45-, 43-, 39-, and 35-kDa proteins, which are already known as rat liver cytokeratins, excepted the 64- and 35-kDa bands. This antibody can therefore be considered to be a pan-cytokeratin marker for the rat. A 51-B/H4 detected an epitope occurring in both lamins (70, 67, and 60-63 kDa) and cytokeratins (49, 45, 43, and 39 kDa). In a few experiments, this monoclonal antibody also indicated a 55-kDa protein. Furthermore, in some cell lines a 80-kDa protein was detected which possibly may correspond to a higher molecular member of the lamin family. In subcellular preparations, the full set of cytokeratins was found to be associated with the nuclei and the nuclear lamina fraction, suggesting a tight connection of the intermediate filaments with the nuclear lamina. Cell lines derived from fetal or neonatal rat liver exhibited cytokeratin patterns which resembled the phenotype of immature or atypically differentiated parenchymal liver cells.
Animals↗
[Immunohistochemical studies of bronchial carcinoma using monoclonal antibody A63-C/A3].
An immunohistological study was undertaken, using paraffin sections of 22 cases of lung carcinoma and a number of reference tissue samples, to clear up the reactivity of A63-C/A63 monoclonal antibody (IgM) which was capable of identifying a carcinoma-associated carbohydrate antigen. Following neuraminidase pretreatment of those sections, positive response to the above antigen was produced by more than 90% of all bronchial carcinoma samples, notwithstanding the histological type involved (squamous-cell carcinoma or adenocarcinoma). Columnar epithelium of the bronchi, not involved in the neoplastic process gave a positive immunoreaction with the antibody A63-C/A3 in 64% of the cases.
Adenocarcinoma↗
Production and characterization of monoclonal antibodies against carcinoembryonic antigen (CEA).
In the experiments presented here 22 monoclonal antibodies (MoAbs) were produced which reacted with the tumor marker carcinoembryonic antigen (CEA). Eleven of the MoAbs reacted neither with peripheral blood granulocytes nor with purified spleen NCA-60 kDa and were therefore regarded as "CEA-specific". Only three antibodies of this group reacted exclusively with CEA-180 kDa. Eight MoAbs reacted with CEA-180 kDa and with CEA-like substances of lower molecular mass (of 160 kDa and/or 120 kDa) present in colon carcinoma cells as determined by immunoblotting. These molecules seem to be different from the classical non-specific cross-reacting antigens (NCAs) present in peripheral blood granulocytes. In contrast to that, the other 11 anti-CEA MoAbs recognized in addition to CEA-180 kDa also NCAs on granulocytes. Six of them were reactive with a purified spleen NCA-60 kDa preparation. These MoAbs bound also to reduced and alkylated CEA-180 kDa (CEA r/a), i.e. they recognize sequential epitopes. All 22 MoAbs reacted with CEA expressed in different human tumor cell lines as determined by immunocytological analysis. But six of them did not bind to the surface of these cells when tested in a radioimmuno-binding assay. It was concluded that the epitope(s) recognized by these antibodies are involved in cell membrane anchoring of the CEA-molecules.
Antibodies, Monoclonal↗
Expression of cytokeratins 8 and 18 in human Sertoli cells of immature and atrophic seminiferous tubules.
Tissues of human testes, either normal (23 specimens of various developmental stages), or affected by pathological conditions (19 specimens of dystopia, atrophia and/or oligospermia) were immunohistochemically examined for the expression of different cytokeratins, using mainly frozen material. Cytokeratins 8 and 18 were found in varying amounts in Sertoli cells of fetal, prepubertal and senile testes and in all cases of pathological alteration. Cytokeratins were completely absent only in normal, mature seminiferous tubules. Therefore, the immunohistochemical detection of cytokeratins in Sertoli cells seems to provide a sensitive marker for immature or damaged testes.
Adolescent↗
Expression of cytokeratin 19 during human liver organogenesis.
Immunohistochemistry with monoclonal anti-cytokeratin antibodies has revealed the presence of cytokeratin 19 in embryonic and early fetal hepatocytes. With the differentiation of bile ducts at about the 10th week, cytokeratin 19 disappears from liver cells but remains in bile duct cells. This marks an important step in the organogenesis of the liver.
Antibodies, Monoclonal↗
[Cytokeratin expression of tumor tissue in cutaneous merkeliomatosis (disseminated cutaneous Merkel cell carcinomas)].
In 2 patients suffering from cutaneous merkeliomatosis, we investigated the immunohistochemical expression of cytokeratin in cutaneous tumors, making use of 3 monoclonal antibodies. We observed a strong selective cytoplasmic fluorescence of the tumor cells in formalin-fixed tissue sections. Thus we were able to detect single intra-epidermal tumor cells. Both these findings and the intracorneal occurrence of tumor cells argue for a transepidermal elimination process. The reactivity patterns with monoclonal anticytokeratins revealed a cytokeratin expression of simple epithelial including cytokeratin 19.
Antibodies, Monoclonal↗
[Introduction and results in the endoscopic treatment of extrauterine pregnancy].
After having applied endoscopic treatment of ectopic pregnancy for one year we herein report on 50 patients who were treated either by laparoscopy (in 20 cases) or laparotomy (in 30 cases). Conservative endoscopic surgery was performed in 12 cases (9 salpingotomy, 2 endoscopic restoration, 1 partial resection of the ovary) while conservative surgical methods by laparotomy were applied in 13 cases (8 milk out, 3 milk out with fimbrioplasty, 1 salpingotomy, 1 partial resection of the ovary). Partial endoscopic salpingectomy was necessary in 8 cases. Out of the women with laparotomy management 4 underwent an adnexectomy and 13 a salpingectomy. After laparoscopic treatment of ectopic pregnancy two patients developed an intrauterine pregnancy and in one case an ectopic pregnancy was observed again. In the laparotomy-group no intrauterine or ectopic pregnancy recurred in the period under review. There was no complication after laparoscopic management of ectopic pregnancy. However a temperature above 38 degrees C occurred in 5 cases after laparotomy caused by local inflammation or by post-operative infiltration. After endoscopic salpingostomy patency of the fallopian tubes was proofed by hysterosalpingography in 6 cases, and all tubes were found to be patent. Endoscopic management of ectopic pregnancy is recommended due to low post-operative morbidity rates and short time of hospitalization.
Fallopian Tube Patency Tests↗
Patterns of expression of tumor markers on non-transformed human mammary epithelial cells cultured in vitro.
We have examined the expression of 7 well defined tumor markers/tumor associated antigens (H type 2, X, Y, sialyl-Lea, CEA, MAM-6, and Tn) and a tumor associated antigen defined by a new own monoclonal antibody on non-transformed human epithelial cell lines derived from reduction mammoplasties by means of immunocytochemistry with monoclonal antibodies. Two cell types are discernible: slowly or non-proliferating, lumenal derived (I), and proliferating, stem cell-like, basal cell-derived cells (II). Five out of the 8 tumor markers were expressed on type I cells, and all 8 on type II cells. The number of positive cells varied considerably from a few to 100 per cent depending on the individual markers. The observed patterns proved to be characteristic and reproducible; they appear to reflect the developmental stage of the cells cultured in vitro rather than a direct influence of the culture conditions.
Biomarkers, Tumor↗
EDTA-polyvinylpyrrolidone as a preservation medium for immunohistochemistry.
In a solution on 7% EDTA and 5% polyvinylpyrrolidone in 0.1 mol/l. Tris buffer, pH = 6.95, tissues samples can be stored at room temperature for 1 week without detectable loss of structural integrity or antigenicity (as demonstrated in immunohistochemistry with monoclonal antibodies to intermediate filament proteins).
Biomarkers, Tumor↗
Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin.
We applied immunohistochemical techniques and gel electrophoresis to examine the distribution of intermediate filaments in human fetal oral epithelium and the epithelia of the human enamel organ. Both methods demonstrated that human enamel epithelia contain cytokeratins 5, 14, and 17, which are typical of the basal cells of stratified epithelia, as well as smaller quantities of cytokeratins 7, 8, 19, and in trace amounts 18, which are characteristic components of simple epithelial cells. In the external enamel epithelium and stellate-reticulum cells, most of these components appeared to be simultaneously expressed. In contrast, the parental oral epithelium was negative for cytokeratin 7, thus indicating possible "neoexpression" during the course of tooth formation. Immunohistochemical procedures using various monoclonal antibodies against vimentin revealed the transient coexpression of vimentin and cytokeratins in the external enamel epithelium and in stellate-reticulum cells during enamel development. The significance of the coexpression of cytokeratins and vimentin is discussed in relation to previous findings obtained in other normal tissues and in the light of the functional processes characteristic of these epithelia.
Antibodies, Monoclonal↗
Age-related changes in the expression of cytokeratin and vimentin in human choroid plexus.
Using immunohistochemical methods the expression of intermediate filaments in fetal and adult human choroid plexus epithelium was investigated. The results indicate that the full expression of cytokeratin and vimentin was seen at the 22nd gestational week corresponding to the onset of liquor production. In neural tube and crest cells of human embryos no cytokeratins were detectable, whereas vimentin could be demonstrated in early stages.
Aging↗
Coexpression of cytokeratin and vimentin in Rathke's cysts of the human pituitary gland.
Immunohistochemistry with monoclonal and polyclonal antibodies revealed the presence of cytokeratins in epithelial cells of Rathke's cysts in the pars intermedia of the human pituitary gland. With monoclonal antibodies specific for individual cytokeratins, the expression of CK 18, CK 8, CK 7, and CK 19 could be shown in these cells. Within the hypophysis, CK 19 and CK 7 were restricted to Rathke's cysts and a few epithelial cell clusters in the pars tuberalis, whereas other cytokeratins were also present in endocrine cells of the pars distalis. Furthermore, vimentin and, focally, glial fibrillary acidic protein (GFAP) were detected in the cystic epithelia. By double labelling, coexpression of cytokeratin and vimentin, GFAP and cytokeratin, and GFAP and vimentin could be demonstrated. Compiled data of all known cases of coexpression of cytokeratin and vimentin in normal cells reveal physiological correlations and suggest a functional significance of this rare type of coexpression of intermediate filament proteins.
Aged↗
Patterns of cytokeratin and vimentin expression in the human eye.
We studied the expression of the various cytokeratin (CK) polypeptides and vimentin in tissues of the human eye by applying immunocytochemical procedures using a panel of monoclonal antibodies as well as by performing biochemical analyses of microdissected tissues. Adult corneal epithelium was found to contain significant amounts of the cornea-specific CKs nos. 3 and 12 as well as CK no. 5, and several additional minor CK components. Among these last CKs, no. 19 was found to exhibit an irregular mosaic-like staining pattern in the peripheral zone of the corneal epithelium, while having a predominantly basal distribution in the limbal epithelium. Both the fetal corneal epithelium and the conjunctival epithelium were uniformly positive for CK no. 19. In the ciliary epithelium, co-expression of CKs nos. 8 and 18 and vimentin was detected, whereas in the retinal pigment epithelium, CKs nos. 8 and 18 were dominant. The present data illustrate the remarkable diversity and complexity of CK-polypeptide expression in the human eye, whose significance with respect to histogenetic and functional aspects is, as yet, only partially clear. The unusual distribution of CK no. 19 in different zones of the corneal epithelium may be related to the specific topography of corneal stem cells. The occurrence of the expression of simple-epithelium CKs in the ciliary and pigment epithelium demonstrates that, despite their neuroectodermal derivation, these are true epithelia.
Ciliary Body↗
Immunohistochemical demonstration of cytokeratin 19-positive basal cells in psoriatic plaques.
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Epidermis↗