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Biomedical subjects

U Junge

Publications and source records attributed to U Junge.

At least 37 records · Page 2Linked to original sources
PubMed1981

Trophic effect of protal blood in maintenance cultures of adult rat hepatocytes.

The hepatotrophic effect of portal serum was demonstrated in primary monolayer cultures of adult rat hepatocytes: DNA synthesis in cultures incubated for 4 days with pooled portal serum of fed rats was significantly higher than in cultures with peripheral serum from the same animals. This difference was not observed when portal blood was treated with charcoal or obtained from fasting rats. Portal serum from diabetic rats stimulated DNA synthesis less than portal control serum. The trophic effect of portal serum from normal rats was enhanced after tolbutamide injection. The lost tropic effect of charcoal-treated portal serum was completely restored when insulin was added to reobtain a normal portal immunoreactive insulin concentration. Thus, insulin seems to be the main factor responsible for the hepatotrophic action of portal blood in vitro.

Animals↗
PubMed1981

Solid-phase radioimmunoassay for detection of alcoholic hyalin antigen (AHAg) and antibody (anti-AH).

Alcoholic hyalin (AH) was isolated and purified from post-mortem livers of patients with alcoholic hepatitis. Antibodies against AH (anti-AH) were raised in guinea-pigs. Their specificity was demonstrated by immunofluorescence and by immunoabsorption. The antisera were positive in immunofluorescence and complement fixation up to serum dilutions of 1:320 and 1:32 respectively. In order to achieve a higher sensitivity a solid-phase radioimmunoassay (SP-RIA) was established for detection of alcoholic hyalin antigen (AHAg) and anti-AH. Anti-AH could thus be measured up to a 10(5) serum dilution. Using a blocking test, solubilized AH at protein concentrations as low as 80 to 160 ng/ml could be detected. With this SP-RIA, neither AHAg nor anti-AH was found in the sera of 32 patients with histologically proven alcoholic hepatitis. The sera were likewise negative by indirect immunofluorescence, complement fixation and immune adherence haemagglutination test.

Antibodies↗
PubMed1977

Hepatotrophic effects of pancreatic and gastrointestinal hormones in the rat in vivo and in vitro.

The role of gastrointestinal and pancreatic hormones in regulating liver growth was evaluated by measuring their effect on DNA synthesis in the normal and regenerating liver of rats in vivo and in maintenance cultures of adult rat hepatocytes in vitro. After partial liver resection DNA synthesis reached peak levels after 24 hours while serum concentrations of immunoreactive insulin in portal and peripheral blood at this time were still suppressed. Increase of endogenous insulin levels by intravenous glucose infusion or portal infusion of insulin, glucagon or both together with glucose did not change DNA synthesis in normal or regenerating rat liver. After acute carbon tetrachloride poisoning of rats, survival rate and degree of liver necrosis was not changed by intraperitoneal infusion of glucagon and insulin with glucose. In vitro, insulin, glucagon and somatostatin synergistically stimulated the specific thymidine uptake in seven-day-old maintenance cultures of rat hepatocytes. The hormones did not cause cell multiplication but enhanced cell survival, probably by improving the uptake and utilization of nutrients. Gastrin G-17, secretin and cholecystokinin (contaminated with gastric inhibitory polypeptide) had no effect. It is concluded that the results do not support the contention that liver regeneration is regulated by the known pancreatic hormones. However, a trophic effect of pancreatic hormones on liver cells in vitro could be demonstrated. Gastrointestinal hormones had no such effect.

Animals↗
PubMed1976

[Hepatitis in dialysis wards (author's transl)].

The endemic spread of hepatitis B is a big problem of many hemodialysis wards. Dialysis patients have mild, often anicteric diseases, but the number of chronic types among them with persistent HBs Ag is particularly high. The high proportion of clinically unapparent hepatitis affections and HBs carriers favors the endemic spread of hepatitis B in dialysis wards. By reduction of the number of blood transfusions, by the use of suitable equipment, segregation of HBs Ag positive patients from negative patients and strict maintenance of hygienic rules by patients and personnel the incidence of hepatitis B can be markedly lowered. Passive immunity by means of gammaglobulin containing a high-titer of anti-HBs is possible, but as yet there is no active immunization.

Carrier State↗
PubMed1976

Ground-glass hepatocytes in unselected liver biopsies. ultrastructure and relationship to hepatitis B surface antigen.

400 unselected liver biopsies were stained with aldehyde-thionin (AT) and examined for ground-glass hepatocytes (GGH). 1) AT-positive GGH were found in about half of all HBSAg-positive patients but were never seen in HBSAg-negative patients. 2) AT-positive GGH were observed in healthy HBSAg carriers and in all forms of chronic hepatitis but never in acute HBSAg-positive hepatitis. 3) AT-negative GGH were occasionally seen in HBSAg-negative patients under drug treatment. 4) Ultrastructurally GGH showed proliferation of smooth endoplasmic reticulum which only in AT-positive GGH contained filaments, 20-25 nm in diameter, within its cisternae.

Acute Disease↗
PubMed1975

The effect of an immunosuppressive drug (azathioprine) on the latent infection of marmoset monkeys (Callithrix jacchus) with the oncogenic Herpesvirus saimiri.

Callithrix jacchus (CJ) marmoset monkeys can be latently infected with Herpesvirus saimiri (HVS). In order to determine whether this resistance to the oncogenic potential of HVS could be due to the immune surveillance, azathioprine, a known immunosuppressive drug, was given to 7 latently infected CJ marmosets. The animals died within 147 days probably from side effect caused by azathioprine, but no animal developed a tumor.

Animals↗