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Biomedical subjects

U Hess

Publications and source records attributed to U Hess.

80 records · Page 5Linked to original sources
PubMed1975

[Simple method for obtaining Toxocara canis antigen for the indirect immunofluorescence technic].

A simple method for the preparation of Toxocara canis antigen for the indirect immunofluorescent test is described: Embryonated Toxocara eggs are treated for 12 hours at room temperature with a 1:1 mixture of 2% NaHO and sodium hypochlorite (NaClO) solution with a concentration of 2% free chlorine in order to remove the outer layers of the egg shells. The larvae which are still enclosed in the lipid membrane are freed by mild ultrasonic treatment. Thereafter, the suspension of larvae is washed and purified in a modified Baermann apparatus. In this way large numbers of larvae in pure suspension were gained and used for the production of frozen sections for the indirect immunofluorescent test. Rabbits and mice experimentally infected with embryonated Toxocara canis eggs showed a positive serological reaction (titers between 1/10 to 1/320) in this test with Toxocara larvae as antigen, while in uninfected control animals no antibodies could be detected. The larval antigen exhibited only a weak cross reaction with sera of animals infected with Ascaris suum eggs.

Animals↗
PubMedDate not supplied

[Synthesis of 4-aryl-1-lambda-2, 2-lambda- 4-dithia-3,4-diaza-buta-1,2-dienes, a new dithiadiazabutadiene system].

4-Aryl-1 lambda 2, 2 lambda 4-dithia-3,4-diaza-buta-1,2-dienes 1a-12a as representatives of a dithiadiazabutadiene structure in which sulphur occupies a different oxidation number are synthesized by reaction of diazotated acceptor-substituted aryl- and hetarylamines 1-12 with thiosulfates or disodium disulfide in strong acid solution. For some examples a first pharmacological test indicate an immune-stimulating effect. Analytical examination as well as MNDO calculation which give some mechanistical insight, supplements the new synthesis.

Adjuvants, Immunologic↗