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Biomedical subjects

U Heine

Publications and source records attributed to U Heine.

At least 37 records · Page 2Linked to original sources
PubMed1982

[Ophthalmologic tumor service for the care of patients with malignant intraocular tumors].

The organization of treatment for patients with malignant intraocular tumors is described with reference to the oncology department of an eye clinic specializing in tumor problems. Since eye tumors are seldom encountered in day-to-day ophthalmological practice, and the costs of diagnosis and treatment are so high, centralized treatment facilities are advocated.

Ciliary Body↗
PubMed1981

Exfoliation of membrane ecto-enzymes in the form of micro-vesicles.

Cultures from various normal and neoplastic cell lines exfoliated vesicles with 5'-nucleotidase activity which reflected the ecto-enzyme activity of the parent monolayer culture. The ratio of 5'-nucleotidase to ATPase activity in the microvesicles indicated that cellular ecto-ATPase was conserved in the exfoliative process. Phospholipids of the microvesicles contained significantly increased amounts of sphingomyelin and total polyunsaturated fatty acids. It was concluded that the shedded vesicles constituted a select portion of the plasma membrane. Examination by electron microscopy showed the vesicles had an average diameter of 500 to 1000 nm and often contained a second population of vesicles about 40 nm in diameter. As much as 70% of the plasma membrane ecto-5'-nucleotidase activity of a culture was released into the medium over a 24-h period. Phosphoesterhydrolases from C-6 glioma or N-18 neuroblastoma microvesicles dephosphorylated cell surface constituents when in contact with monolayer cultures. Exfoliated membrane vesicles may serve a physiologic function; it is proposed that they be referred to as exosomes.

Adenosine Triphosphatases↗
PubMed1980

[Epidemiologic studies on the occurrence of hepatitis B in dentists and their assistants in Bavaria].

A study of 586 dentists and 553 dental assistants revealed that the contamination of dentists with hepatitis B was three times higher than in the comparable control group. No elevated hepatitis B morbidity rate however could be determined for the dental assistants. The occupation-related risk of hapatitis B infection was one case for every 100 dentists per year. No serologic differences from the normal control group could be determined for dentists and dental assistants in regard to contamination with hepatitis A. The evaluation of the questionaire showed that most dentists and their assistants were not aware of their hepatitis infection. Protective epidemiologic and hygienic measures, for the most part, were not observed.

Adult↗
PubMed1978

Size and secondary structure of avian myeloblastosis virus associated ribosomal RNA: comparison with cellular and precursor ribosomal RNA.

Ribosomal RNA isolated from ribosomes present inside avian myeloblastosis virus (AMV) was characterized by electron microscopy using the formamide-urea spreading technique. The molecular weight and the secondary structures were compared with those of r-RNA and precursor r-NA isolated from host cells, the leukemic myeloblasts. The molecular weight of viral r-RNA (1.62 +/- 0.18 X 10(6) and 0.69 +/- 0.10 X 10(6)) and the molecular weight of cellular r-RNA (1.63 +/- 0.18 X 10(6) and 0.67 +/- 0.09 X 10(6)), the latter obtained from avian myeloblasts, were found to be identical and comparable with the molecular weight of chicken liver r-RNA. Likewise, the secondary structures of viral r-RNA were identical to those of cellular r-RNA. The postulated possible precursor character of viral r-RNA was excluded, since the molecules of viral r-RNA do not show any similarity to those of precursor r-RNA. Previously observed differences in behavior of viral and cellular (myeloblastic) r-RNA in sedimentation and electrophoretic mobility are discussed.

Animals↗
PubMed1978

Nuclear accumulation of filamentous herpes simplex virus DNA late during the replicative cycle.

New ultrastructural findings within the nucleus of herpes simplex virus-infected cells are illustrated. The occurrence of bundles of tightly packed filaments during the late stages of the infectious process is described. These bundles were found in different areas of the nucleus and were not associated with any nuclear organelles. Employing different staining techniques and high-resolution autoradiography, they could be identified as DNA-containing nucleoproteins. These filaments may be interpreted as a special form of viral DNA accumulation within the nucleus. Changes of these structures toward a more recticular arrangement were observed as soon as the nucleoplasm disintegrated.

Cell Line↗
PubMed1977

Establishment and characterization of a cell line derived from a spontaneous murine lung carcinoma (M109).

The Madison lung (M109) tumor cell line, initiated from a "spontaneous", anaplastic murine lung carcinoma, has been propagated continuously in vitro for more than 300 cell generations. Cytogenetic analysis revealed a mouse karyotype with a mode of 78 chromosomes (2n = 40). Three distinct marker chromosomes were identified by trypsin-giemsa banding. The cells piled up in culture and had a short generation time and high plating efficiency. Electron microscopy revealed highly undifferentiated cells with little rough endoplasmic reticulum, an abundance of free polysomes, the presence of few and often odd-shaped mitochondria, lipid bodies and phagocytic vacuoles. Virus particles of the C-type were found frequently. The subcutaneous transplantation of M109 cultured cells at a relatively low cell inoculum produced highly metastatic tumors in syngeneic BALG/c mice.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMed1976

Xenotropic properties of an isolate from murine Rauscher leukemia virus in primates.

Murine Rauscher leukemia virus (MuRLV) from BALB/c plasma consisting of a mixture of an ecotropic and a xenotropic virus could be separated out by a selection process when propagated in human and simian cell cultures. This hypothesis is supported by obtaining consistently lower infectivity titers of human cell propagated RLV in human and simian cells as compared to MuRLV propagated in mouse cell cultures. Furthermore, RLV passaged in a simian cell culture failed to replicate in mouse cells, had a wide host range, was able to rescue Moloney sarcoma genome, possessed murine type C group-specific antigen, and was neutralized by anti-HRLV. Its reverse transcriptase was strongly inhibited by antiserum to MuRLV enzyme; however, antiserum to woolly monkey enzyme also inhibited (30%) its reverse transcriptase, suggesting some difference in antigenic properties. Inoculation of this virus in rhesus monkeys was inconclusive.

Animals↗
PubMed1976

Renal neoplastic response to leukosis virus strains BAI A (avian myeloblastosis virus) and MC29.

Previous reports described the induction of avian renal neoplasms by leukosis virus strains BAI A [avian myeloblastosis virus (AMV)] and MC29, and illustrated morphological characteristics of the tumors. Continued studies in this work confirm evidence of the origin of the tumors from embryonal cells residual in the posthatched chick. The work further emphasizes differences in histopathology of the neoplasms caused by the two viruses and reveals differences in the histopathogenesis of the respective growths. Embryonal rests may consist of two types of cells, those of epithelial characteristics and a second element of differentiation between nephroblastema (mesenchyme) and epithelium and designated here as nephromesoblastoma. Infection by AMV induces tumors of epithelial characteristics and, in addition, derivatives of nephromesoblastoma consisting of cartilage, bone, areas of keratinization, and sarcoma. Keratinized structures in the nephroblastoma originate from nephromesoblastoma. In contrast, MC29 virus induces only epithelial growths representing principally aberrant and malformed glomerular and tubular structures with occasional cartilage derived from epithelial cells. MC29 tumors are completely lacking in nephromesoblastoma tissue and contain no bone, sarcoma, or keratinized formations. In MC29 tumors, occasional cartilage was derived from epithelium. Tumors caused by AMV exhibit the complex structure of nephroblastoma with all of the features of the growth in humans (Wilms' tumor). The neoplasms induced by both AMV and MC29 exhibit marked aberration, distortion, and malformation in the differentiation of the cells growing out from the embryonal rests representing rare manifestations of cell genetic influence inherent in the primordial growth of nephroblastema. The results thus illustrate fundamental differences in cellular composition and capacity to respond to etiologically different leukosis viruses.

Adenocarcinoma↗