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Biomedical subjects

U Hadding

Publications and source records attributed to U Hadding.

121 records · Page 7Linked to original sources
PubMed1967

Complement: substitution of the terminal component in immune hemolysis by 1,10-phenanthroline.

The participation of metal ions in the terminal step of immune cytolysis was investigated with chelating agents. 1,10-Phenanthroline induced lysis of sensitized sheep erythrocytes which had reacted with the first eight components of human complement. Hemolysis caused by 1,10-phenanthroline resembled lysis produced by the ninth component of complement in dependence on cell-bound eighth component and on temperature and in inhibition by 0.09M ethylenediaminetetraacetic acid. Bivalent metal ions reduced the hemolytic capacity of 1,10-phenanthroline, and Fe(++) inhibited the activity of the ninth component. Since trivalent iron had no such effects, it is postulated that the hemolytic activity of 1,10-phenanthroline and the ninth component of complement is a function of their affinity for Fe(++).

Animals↗
PubMedDate not supplied

Cloning and expression of P60, a conserved surface-localized protein of Mycoplasma hominis, in Escherichia coli.

The clp60 gene encoding P60, a conserved lipoprotein of Mycoplasma hominis, was cloned and sequenced from both the type strain PG21 and the isolate FBG. Both open reading frames were identical in length, comprising 1746 nucleotides. The deduced amino acid sequences differed in 16 out of 582 amino acids. As expected, none of these divergences mapped within the epitope that was recognized by mAb CG4 in all of the 198 isolates of M. hominis analyzed so far. This conserved epitope was narrowed down to amino acids 454 through 464 within the C terminus of P60. For the expression of the recombinant homolog P60, P60rec, in E. coli the TGA codons of clp60 were substituted for TGG codons prior to cloning of clp60 into the expression plasmid pQE41. The expression of P60rec as a fusion protein with dihydrofolate reductase carrying an N-terminal His-tag enabled the purification of large amounts of P60rec in a soluble form.

Amino Acid Sequence↗