Search PubMed⌕ Search

Biomedical subjects

U Dietrich

Publications and source records attributed to U Dietrich.

At least 91 records · Page 5Linked to original sources
PubMed1987

EGF homologous sequences encoded in the genome of Drosophila melanogaster, and their relation to neurogenic genes.

The function of the neurogenic genes of Drosophila melanogaster is required for a normal pattern of commitment of neural and epidermal progenitor cells. In the course of searching for a molecular basis for the functional interrelationships that exist between the neurogenic genes, fragments of cloned DNA from the genes master mind (mam), Delta (Dl), Enhancer of split [E(spl)] and Notch (N) were hybridized to each other. Strong cross-hybridization was observed between a fragment of the Dl gene and a fragment of the N gene encoding a peptide with homology to several proteins of mammals, including the epidermal growth factor (EGF). Sequencing of this Dl fragment revealed an open reading frame encoding four EGF-like repeats with homology to the repeats found in the N gene. Screening genomic and cDNA libraries under conditions of reduced stringency with Dl and N probes that encode EGF-like repeats uncovered several cross-hybridizing clones, suggesting that other Drosophila genes may also encode such peptides. Part of a cross-hybridizing cDNA clone, derived from a gene located at position 95F on the third chromosome, was sequenced and found to encode five repeats with homology to those encoded by N and Dl. Preliminary evidence on the spatial pattern of transcription indicates that the gene at position 95F is regulated in its expression, as it is transcribed in all ectodermal derivatives, with the exception of the central nervous system. Indirect evidence suggests that this clone may derive from the crumbs (crb) gene, which is likely to be an hitherto unknown neurogenic gene.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Sequence↗
PubMed1984

The expression of neurogenic loci in imaginal epidermal cells of Drosophila melanogaster.

Six zygotically expressed genomic loci of Drosophila melanogaster, N, bib, mam, neu, Dl and E (spl), have previously been found to be involved in the commitment of ectodermal cells as neuroblasts. We have studied phenotypes induced by various mutant alleles at these 6 loci in imaginal epidermal cells, in order to assess possible implications of the genes in functions other than early neurogenesis. When homozygous, any of these mutations except bib affected the development of bristles and compound eye in various ways. These range from cell death to the production of additional bristles and several defects in ommatidial patterning, depending on the allele used. In contrast clones of bib homozygous cells exhibited wild-type phenotypes. The differences found in the expression of the neurogenic genes are discussed with respect to a hypothesis on the genetic control of neurogenesis.

Alleles↗
PubMed1983

Computer tomographic long-time results after surgical removal of subdural fluid collections in infancy and childhood.

61 infants and children underwent surgery of acute hygroma, acute subdural hematoma, chronic subdural hygroma, chronic subdural hematoma, and subdural empyema. Long time-follow up including computerized tomography could be performed in 20 of these cases. Only 3 CT-scans had been without pathological finding. The most common finding had been an enlargement of the inner and outer CSF spaces suggesting cerebral atrophy. No correlation had been found to kind of surgical treatment and clinical status.

Adolescent↗
PubMed1977

[Mycobacterial infections in internal medicine patients].

It is reported on four own observations of atypical mycobacteriosis, which had been evident within the clinical examinations of a larger mixed number of patients with internal diseases. In three patients pathogenic significance must be ascribed to the proved atypical mycobacteria. All the three had other basic diseases. Only in one patient it appeared as a purely pulmonary form of manifestation. On the basis of a further observation is shown that a single proof of atypical mycobacteria may be regarded only as accidental findings. The clinical symptomatology of this group of diseases is multiform. The differential-diagnostic considerations are usually determined by the basic disease. As apart from this the proof of atypical mycobacteria is difficult, infections with these microorganisms might at present not infrequently remain infrequently. The observation of a disease by mycobacterium xenopi emphasizes that also in this microorganism good chances of success may be given by medicamentous antituberculotic therapy.

Humans↗
PubMed1977

[Pulmonary metastasizing of prostatic carcinoma--source of errors in the ambulatory x-ray examination of lungs].

It is reported on eight patients with a primary carcinoma of the prostatic gland who were admitted to the hospital for clinical clarification of conspicuous pulmonary findings. The radiological findings of a general radiograph of the thorax are multiform and for themselves alone they do not permit an etiological coordination. For this reason the demand can be derived in patients of the adequate age group with tumour symptomatology can also be thought of a carcinoma of the prostatic gland and already during the examination in the outpatient department to carry out a palpatory examination and, if possible, to make a general radiograph of spinal column and pelvis, since in these cases the diagnosis can be made already before admission.

Bronchography↗
PubMedDate not supplied

Molecular cloning of two west African HIV2 isolates that replicate well in macrophages.

HIV2 strains were isolated from a Gambian with neuro-AIDS (HIV2D194) and from an asymptomatic Ghanian (HIV2D205). Like HIV1 biological subtype c, both isolates grew slowly and induced few or no syncytia, but eventually produced high levels of particle-associated reverse transcriptase (RT) in cultures of fresh peripheral blood lymphocytes. Each produced even higher levels of RT in fresh human macrophages, especially HIV2D194, where maximal RT values of 1,800,000 cpm/ml supernatant of approximately 30,000 cells were measured. The viruses were molecularly cloned after a single passage in culture. Restriction site analysis showed heterogeneity within each isolate. Nucleotide sequence analysis of HIV2D194 revealed that, genetically, it is a member of the prototypic HIV2 family, displaying 12% divergence vs. HIV2ROD and HIV2NIHZ. In contrast, HIV2D205 is the most highly divergent HIV2 strain yet described: it is equidistant in relation between the known HIV2 strains and the SIVMAC isolates (23-24% nucleotide sequence divergence).

Africa, Western↗
PubMedDate not supplied

A highly divergent HIV-2-related isolate.

It has been suggested that the human immunodeficiency virus type 2 (HIV-2) and the simian immunodeficiency virus from rhesus macaques (SIVmac) evolved from the sooty mangabey virus SIVsm (ref. 1). We now describe an HIV-2-related isolate, HIV-2-D205, from a healthy Ghanaian woman that is genetically equidistant to the prototypic HIV-2 strains and to SIVsm and SIVmac. Supported by the observation that HIV-2D205 differs in a step of envelope glycoprotein processing, our data indicate that it could represent an alternative HIV-2 subtype and that viruses of the HIV-2/SIVsm/SIVmac group could have already infected humans before HIV-2 and SIVsm/SIVmac diverged.

Base Sequence↗
PubMedDate not supplied

MR imaging of calcified herniated thoracic disk.

The magnetic resonance findings in a 48-year-old woman with a calcified, herniated thoracic intervertebral disk at the T5-T6 level are reported. This patient had an 18 year history of spinal cord symptoms and signs, previously thought to be caused by multiple sclerosis (MS). Magnetic resonance was obtained for further evaluation of MS.

Calcinosis↗