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Biomedical subjects

U Desselberger

Publications and source records attributed to U Desselberger.

140 records · Page 8Linked to original sources
PubMed1975

Relation of virus particle counts to the hemagglutinating activity of influenza virus suspensions measured by the HA pattern test and by use of the photometric HCU method.

The ratios of the number of virus (n.v.p.) to the hemagglutinating activity were determined for a series of influenza A and B strains using both the photometric HCU method and the HA pattern test. The logarithms of the ratios of n.v.p./hcu ranged from 7.06 to 7.54 and the logarithms of the ratios of n.v.p./HA from 6.87 to 7.56. While the n.v.p./HA ratios of different strains showed significant differences in a number of cases, such differences were not found for the n.v.p./HCU ratios with the only exception of the strain B/Hong Kong/8/73 differing uniformly from the other strains tested. Since the standard deviation of HCU values ranged from 0.006 to 0.039 log units and that of HA titers from 0.076 to 0.272 log units, it was concluded that the differences of n.v.p./HA ratios found were due to the range of variance of HA tests and did not reflect strain specific differences. The degree of purity of virus suspensions was found not to influence measurably the log n.v.p./HCU values.

Hemagglutination Tests↗
PubMed1975

[On the antigenicity of influenza virus aluminum oxide adjuvant vaccine "Alorbat" in man (author's transl)].

The antihemagglutinin (AH) and antineuraminidase (AN) antibody response in 35 humans to a single vaccination with the aluminum oxide adsorbed influenza virus vaccine "Alorbat" was investigated. The vaccine contained the strains A/Hong Kong/1/68 (H3N2), A/Hong Kong/107/71 (H3N2), A/England/42/72 (H3N2) and B/Iowa/1/69. the rates of significant AH antibody titer increase ranged from 51 percent (against A/Hong Kong/68), 69 (against A/Hong Kong/71), 63 (against A/England/72) to 83 percent (against B/Iowa/69). Persons with high prevaccination titers failed to yield postvaccination antibody titer increase (table 1). Antibody titer increase measured against the neuraminidase of A/England/72 virus was recorded in 60 percent and against the neuraminidase of B/Iowa/69 virus in 31 percent (table 2). Using the neuraminidase of the recombinant strain (A/Bel/42 (H0)-A/Sing/57 (NS) as test antigen a significant titer increase in 32 percent of sera tested was found (table 2). The AN antibody titers against A/England/72 exceeded the titers against the A/Bel-A/Sing recombinant 3,67-5,42 fold. This finding was shown to be caused by additional reaction of anti-H3 antibody with A/England/72 virus resulting in steric hindrance of neuraminidase activity.

Adjuvants, Immunologic↗
PubMed1974

Kinetics of inhibition of influenza virus hemagglutination by homologous antihemagglutinin and antineuraminidase antibodies as measured by means of the photometric antibody concentration unit method.

The kinetics of the reactions of antihemagglutinin (AH) and antineuraminidase (AN) antibodies with homologous influenza virus were examined by use of a photometric hemagglutination inhibition test (antibody concentration unit [ACU] test). The following results were obtained. (i) The isotherms describing the reaction of AN antibodies with homologous virus were found to have much steeper slopes than did the corresponding AH antibody isotherm. This finding indicates that the photometric ACU method can discriminate objectively between AH and AN antibodies. (ii) The reaction of mixtures of AH and AN antibodies with homologous virus was examined and found that AH antibodies combine with virus independently from the presence of AN antibodies, whereas AH antibodies were found to reduce greatly the measurable degree of hemagglutination inhibition by AN antibodies. (iii) A formula was developed and experimentally confirmed by means of which the binding of virus by mixtures of AH and AN antibodies can be predicted. Thereby, the influence of the relative concentration of AH and AN antibodies on the establishing of isotherms and on their subsequent use for antibody titration by means of the photometric ACU method was investigated. It was found that the procedure routinely employed for establishing isotherms yielded data reflecting the reaction of AH antibodies only. As a consequence, the use of these isotherms will identify AH antibodies.

Antibodies, Viral↗
PubMedDate not supplied

Determination of rotavirus serotype-specific antibodies in sera by competitive enhanced enzyme immunoassay.

A method is described for the specific detection of antibody to individual rotavirus serotypes in sera. A competitive enzyme immunoassay (EIA) was developed in which rotavirus serotype-specific monoclonal antibodies against VP7 compete with antibodies in test sera for rotavirus serotype-specific antigen bound to a solid phase. There was an excellent correlation between serotype-specific EIA results and serotype-specific neutralization titres (r = 0.915, P = less than 0.001). The value of this method for rotavirus epidemiology and vaccine trials is discussed.

Antibodies, Monoclonal↗