Immunostaining of mesothelial cells: no reactivity with HLE-1.
Explore the source record for details and available documents.
Antigens, Differentiation↗
Biomedical subjects
U Costabel
Publications and source records attributed to U Costabel.
Lymphocyte subpopulations in malignant ascites of serous papillary ovarian adenocarcinoma. An immunocytochemical study.
The aim of this study was to investigate lymphocyte subpopulations in 17 patients with malignant ascites due to serous papillary adenocarcinoma of the ovary. Eight patients had not been treated prior to the study whereas nine patients had been treated by surgery and chemotherapy. A panel of monoclonal antibodies against surface markers that correlate with the immune functions of the lymphocytes was used. The lymphocyte subpopulations were identified by the immunoperoxidase adhesive slide assay, and the results in treated and untreated patients were compared. Both groups of patients showed lymphocytosis (41 +/- 25% and 33 +/- 14% of the total cells, respectively). The untreated patients had a significantly higher proportion of B cells (14 +/- 4% of lymphocytes) than did treated patients (7 +/- 2%). No differences were found between both groups regarding the helper-inducer/suppressor-cytotoxic T lymphocyte ratio. The proportion of lymphocytes expressing interleukin-2-receptors was higher in treated patients (6 +/- 2%) than in untreated patients (1.2 +/- 1%). Both groups showed a high percentage of natural killer/cytotoxic cells (17 +/- 7% and 18 +/- 5%, respectively). In the only chylous effusion in this study, there was an increase in helper-inducer and activated T lymphocytes. Future studies are required to document whether surface marker analysis of lymphocytes in malignant effusions may be useful for assessment of the prognosis and the results of treatment.
Adult↗
The value of the immunoperoxidase slide assay in the diagnosis of malignant pleural effusions in breast cancer.
Whether immunocytochemical studies of malignant pleural effusions due to breast cancer would increase the diagnostic yield as compared with conventional effusion cytology was examined in 30 cases with biopsy-proven metastatic spread to the pleura. Conventional cytology was performed on air-dried smears as well as on cytocentrifuge preparations stained with the May-Grünwald-Giemsa stain. Immunocytochemistry was performed with monoclonal antibodies against carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA) and human leukocyte antigen (HLA) and the peroxidase-antiperoxidase technique on glass slides after Ficoll-Hypaque centrifugation. By conventional cytology, 13 cases (43%) were positive for malignant cells, 6 cases (20%) were suspicious, and 11 cases (37%) were negative. In marked contrast, all 30 cases were immunocytologically positive for malignancy. Tumor cells in all cases demonstrated a positive reaction for EMA. Some mesothelial cells were also positive for EMA, but their reaction pattern was clearly distinguishable from that of the tumor cells. Twenty-one cases (70%) also showed CEA-positive tumor cells; mesothelial cells never reacted with CEA. Some tumor cells showed a loss of HLA expression. In conclusion, this immunocytologic method can be recommended as a routine procedure for greatly increasing the diagnostic yield of cytology in pleural effusions due to breast cancer.
Breast Neoplasms↗
Multinucleated cells in bronchoalveolar lavage.
Explore the source record for details and available documents.
Bronchi↗
The immunoperoxidase slide assay. A new method for the demonstration of surface antigens on bronchoalveolar lavage cells.
The immunoperoxidase slide assay (ISA) is described as a new method for the demonstration of surface markers on bronchoalveolar lavage (BAL) cells. This technique makes the unlabelled antibody enzyme method using the peroxidase-antiperoxidase (PAP) immune complex for labelling suitable for the evaluation of single cells. By this method, viable cells are firmly attached to poly-L-lysine coated reaction areas of siliconized glass slides. The ISA technique was found to be very useful for testing a battery of monoclonal antibodies against surface antigens on BAL cells from one specimen in parallel. This is due to the low amount of cells needed for this method. Other advantages compared to immunofluorescence techniques are the reduced costs, the possibility of a permanent documentation of the staining on the glass slide preparations, the higher sensitivity and the well preserved morphology of cells. Normal values for lymphocyte markers (OKT3/Leu-1, OKT4/Leu-3a, OKT8/Leu-2a, Leu-7, B1) and monocyte/macrophage markers (OKIa, anti-Mono, anti-IgG) are reported.
Adolescent↗
Effect of storage time on the analysis of lymphocyte subpopulations in pleural effusions.
It is not known how long cell surface antigens can be detected on lymphocytes in pleural effusions. Therefore, the lymphocyte subpopulations of 15 native pleural effusions were analyzed after different storage times, at either 4 degrees C or room temperature, using the peroxidase-antiperoxidase adhesive slide assay. No significant differences in the lymphocyte subpopulations were observed after one day of storage under both conditions, although the immunoreactivity with CD4 was poor in the majority of cases stored at 4 degrees C and in two cases stored at room temperature. After three days of storage at 4 degrees C and after four days of storage at room temperature, a marked decrease in lymphocytes attached to the slides was observed. Immunoreactivity with CD8, CD20, CD45 and HLA-1 was well preserved, also, after one week of storage. Reactivity with CD3 was weak or poor after three days of storage in some cases. It is important to recognize that the preservation of the immunoreactivity of lymphocytes is dependent not only on the nutritive quality of pleural fluids but also on the cell preparation method.
Antigens, CD↗
Immunocytochemical characterization of isolated human type II pneumocytes.
The expression of surface antigens on human type II pneumocytes is unknown but may be important in diagnostic cytology of bronchoalveolar lavage specimens. Thus, the immunocytochemical reactivity of isolated human type II pneumocytes was determined using a panel of commercially available monoclonal antibodies (MAbs). Type II pneumocytes were isolated from fresh human lung tissue obtained from surgical specimens (four non-smokers, six heavy smokers) after enzymatic digestion with dispase and subsequent discontinuous metrizamide gradient centrifugation. MAbs OKIa; EMA; OKT9; BMA 130a, b and c; EP4; TAG 72; HEA 125; and Leu M1 were studied using the peroxidase-antiperoxidase adhesive slide assay method. In all cases, type II pneumocytes reacted positively with OKIa, BMA 130a, BMA 130b, EMA, EP4, TAG 72 and HEA 125 and negatively with OKT9, BMA 130c and Leu M1. The percentage of positively reacting type II pneumocytes was 90 for OKIa, HEA 125 and EP4; 80 for EMA; 50 for TAG 72 and BMA 130a; and 5 for BMA 130b. Human type II pneumocytes share the expression of several antigens with epithelial tumor cells. This limits the usefulness of these markers with respect to differentiating between reactive type II pneumocytes and malignant cells.
Adenocarcinoma, Bronchiolo-Alveolar↗