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Biomedical subjects

U Bucher

Publications and source records attributed to U Bucher.

At least 55 records · Page 3Linked to original sources
PubMed1978

Red cells and gelatin as the core of a unified program for the national procurement of blood components and derivatives. Prediction, performance, and impact on supply of albumin and factor VIII.

The use of a gelatin plasma substitute as an integral part of a unified national program for the procurement of blood components and derivatives paves the way for the extensive use of red cell concentrates in the treatment of hemorrhage, the plasma from the original donations being partly diverted to the fractionation program of a national transfusion service. We estimated the potential of such a system paced by the demand for red cells and predicted that the use of 20% of fresh whole blood and 80% concentrates would not increase the demand for albumin, the yield of which would suffice to cover three-quarters of current needs in our country. The actual performance of a program using 85% of red cell concentrates in a university hospital with an exceptionally high incidence of major hemorrhage was analyzed in detail. The use of albumin per unit of transfused red cells rose by 9%. With a nationwide increase of this magnitude, 67% of the demand in our country could still be met and the estimated true need for albumin of 200 kg per million inhabitants and year would be fully covered. The yield of factor VIII is 200--300% of the demand. The determinants of the effects of such a system vary from country to country, but its fundamental potential is obviously considerable.

Blood Proteins↗
PubMed1977

[The effect of various cryoprotective agents on the human bone marrow].

The following combinations were evaluated as cryoprotectors of human CFU-c enriched bone marrow frozen for one hour at -190 degrees C: 10% DMSO, 1% DMSO + 9% dextran (Dx), 1% DMSO + 9% HES, 15% glycerin (Gc), 1% Gc + 9% Dx, 1% Gc+ 9% HES, 5% Gc + 4% glucose. Cell counts and CFU-c content were compared with controls left at 4 degrees C. Highest values were observed with 10% DMSO and with 1% DMSO + 9% Dx. These studies confirm the superiority of DMSO to Gc as a cryoprotector. With the combination of 1% DMSO + 9% Dx the removal of DMSO prior to reinfusion of the marrow is obviated. This should facilitate the clinical use of autologous marrow transfusion.

Bone Marrow↗
PubMed1976

[The clinical significance of granulocyte colonies in bone marrow cultures].

The culture system for in vitro evaluation of "colony forming units - culture (CFU-c)" is briefly outlined. This method offers a new approach to studies of proliferation and differentiation of hemopoietic progenitor cells, especially in disorders of granulopoiesis. From available published data it is evident that quantitation of CFU-c is also an indicator of diagnostic and prognostic value for assessment of various types of leukemia. The CFU-c assay has furthermore been introduced to test the viability and proliferating capacity of cryopreserved bone marrow, especially with a view to possible transfusion of stored autologous bone marrow as an adjuvant to cytostatic therapy.

Bone Marrow↗
PubMed1975

[Familial autoimmune hemolytic animia (AIHA) with negative Coombs test, lymphocytopenia and hypogammaglobulinemia].

A family with several cases of autoimmune hemolytic anemia and dysgammaglobulinemia is described. Two sibling had documented antierythrocyte antibodies, but a third had repeatedly negative Coombs tests. Diminished immunoglobulin-producing cells and a hypogammaglobulinemia were found, but no functional defect of humoral or cell-mediated immunity could be detected.

Agammaglobulinemia↗
PubMed1975

[Erythrocyte transfusion].

Red cells are the most important and usually the only necessary part of transfused whole blood. Transfusion of concentrated red cells involves several advantages for the recipient (decreased volume load, fewer transfusion reactions, better antianemic effect per unit). The plasma which is separated from whole blood during the preparation of packed red cells can be used for fractionation or for the preparation of platelet concentrates and other special products. The increasing need for blood components makes the substitution of whole blood by packed red cells for transfusion purposes mandatory. The only rare indication for whole blood is fresh blood (i.e. blood up to 48 hours after donation). Red cell concentrates with a hematocrit of 70% are particularly suitable for general use, since the viscosity still allows transfusion without difficulties. With regard to quality and storage time of red cells there is no difference between packed cells and whole blood.

Blood Coagulation Factors↗
PubMed1975

[Sea-blue histiocytes and Gaucher-like cells. Occurrence and significance].

Sea-blue histiocytes (SBH) and Gaucher-like cells are macrophages containing a pigment related to or identical with ceroid or lipofuscin. While an occasional cell may be found in the normal bone marrow, large numbers of SBH can be observed in various conditions mainly associated with increased cellular breakdown or with disturbances of lipoid metabolism. We have examined smears and/or sections of bone marrow and spleens of 722 patients with various hematological disorders. In only four instances large numbers of SBH could be found, twice in the bone marrow, once in the spleen and once in both organs. Two of these patients were suffering from immune thrombocytopenia. Gaucher-like cells have a different morphologic appearance although the pigment shows the same cytochemical characteristics as in SBH. Gaucher-like cells are mainly found in patients with chronic myeloid leukaemia and are thought to be related to the increased breakdown of granulocytes. The exact mechanisms responsible for the formation of these pigment macrophages are not yet known. In the author's view it does not seem justifiable to speak of a special "syndrome of the blue macrophages".

Adult↗